| Project/Area Number |
26670097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経発生 / 大脳皮質 / 活性酸素 / 血管発生 / 低酸素 |
|---|
| Outline of Final Research Achievements |
The precise control of neuronal migration and morphological changes during differentiation is essential for neocortical development. We found that the progenitors had higher levels of mitochondrial reactive oxygen species (mtROS) , but these levels significantly decreased with differentiation. A possible candidate modulator of mtROS is the Prdm16 gene, which was identified by microarray analysis. Prdm16 was specifically expressed by progenitors in the ventricular zone, but its Prdm16 expression declined as cells transitioned into NeuroD1-positive multipolar cells. This downregulation of Prdm16 expression was crucial for the appropriate progression of the multipolar phase and was required for normal cellular development. Both reporter assay and mtROS quantification demonstrated that PGC1α is a major downstream effector of Prdm16 and NeuroD1 that is required for the modulation of multipolar phase and its characteristic mode of migration.
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