| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this project, we aimed to explore the possibility of developing a molecular toolkit to confer cell autonomous oscillating property of membrane potential based on the molecular mechanisms of phosphoinositide dependence of a combination of distinct ion channels together with expression of voltage-sensing phosphatase that dephosphorylates phosphoinositides. Mouse HCN2 was coexpressed with Ci-VSP RNA in Xenopus oocyte. G-V curve was shifted by 20 mV leftward with reduced conductance upon activities of Ci-VSP in a reversible manner. This clearly shows that VSP activity can regulate HCN channel thus providing a possibility that combination of VSP and HCN channel will serve as a component of an autonomous oscillating molecular tool kit. Since a shift of G-V curve was variable from cell to cell probably dependent on sensitivity of HCN channel to a level of cAMP, it is needed to test with cAMP insensitive mutant of HCN channel.
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