Analysis of rotational motion of voltage sensor domain based on phosphorescence polarization
| Project/Area Number |
26670103
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Japan Advanced Institute of Science and Technology |
|---|
Principal Investigator |
TSUTSUI Hidekazu 北陸先端科学技術大学院大学, 先端科学技術研究科, 准教授 (30392038)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 電位センサードメイン / 燐光偏光 / 膜電位固定 / 膜電位 / 状態遷移 / 燐光 / 生理学 / 生体分子 |
|---|
| Outline of Final Research Achievements |
To investigate the transition mechanism of the voltage-sensor domain, an experimental setup for detecting segment rotation using phosphorescence polarization was built. The reliable quantitative measurements were not reached due to the two major problems that the membrane becomes remarkably fragile under the low oxygen condition required for the phosphorescence measurement and that it is difficult to correctly estimate the contribution of non-specific binding of phosphorescence dye to membrane. In the future, there is a possibility that it can be dealt with by using a brighter phosphorescent dye or a highly specific labeling technique that reduces the background light by using a total reflection illumination system. In the final year of the research period, we showed that the potential sensor performs global structural change by using environmentally responsive unnatural amino acid. We also clarified some unique properties of divalent cation permeability of CatSper voltage-sensor.
|
Report
(5 results)
Research Products
(23 results)
-
-
-
-
-
[Journal Article] Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.2017
Author(s)
Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda M, and Nagai T
-
Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42398-42398
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane.2014
Author(s)
Jinno, Y., Shoda, K., Rial-Verde, E., Yuste, R., Miyawaki, A., Tsutsui, H.
-
Journal Title
Front Mol Neurosci.
Volume: 7
Pages: 93-93
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 神経細胞の興奮を可視化する2014
Author(s)
筒井秀和
Organizer
第23回 日本バイオイメージング学会学術集会
Place of Presentation
大阪大学（大阪府、吹田市）
Year and Date
2014-09-04 – 2014-09-06
Related Report
Invited
-
-
