| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
To investigate the transition mechanism of the voltage-sensor domain, an experimental setup for detecting segment rotation using phosphorescence polarization was built. The reliable quantitative measurements were not reached due to the two major problems that the membrane becomes remarkably fragile under the low oxygen condition required for the phosphorescence measurement and that it is difficult to correctly estimate the contribution of non-specific binding of phosphorescence dye to membrane. In the future, there is a possibility that it can be dealt with by using a brighter phosphorescent dye or a highly specific labeling technique that reduces the background light by using a total reflection illumination system. In the final year of the research period, we showed that the potential sensor performs global structural change by using environmentally responsive unnatural amino acid. We also clarified some unique properties of divalent cation permeability of CatSper voltage-sensor.
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