Molecular basis of multifunctional expression induced by the mRNA splicing selective in undifferentiated epithelium
| Project/Area Number |
26670104
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
Yamazaki Jun 福岡歯科大学, 口腔歯学部, 教授 (50230397)
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| Co-Investigator(Kenkyū-buntansha) |
八田 光世 福岡歯科大学, 口腔歯学部, 准教授 (30344518)
岡村 和彦 福岡歯科大学, 口腔歯学部, 准教授 (00224056)
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| Research Collaborator |
KATO Kenichi 福岡歯科大学, 口腔歯学部, 非常勤講師 (90320332)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | スプライシング / 上皮細胞 / クロライドチャネル / 分化 / エピジェネティックス / ミニジーン / 表皮 / 3次元培養 |
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| Outline of Final Research Achievements |
This project aims to clarify the mRNA splicing mechanism in which Ca2+-activated Cl- channel modulator CLCA is switched to its truncated isoform (CLCA-t). Using the splicing reporter plasmids including the gene region (minigene) of exons 8, 9 and 10, the splicing pattern of which is distinct between two CLCA isoforms, the splicing pattern observed in CLCA-t (exon 8+10) was shown to be attenuated by the differentiation of epithelium. Next, we examined the epigenetic regulation of the selective splicing of CLCA in the 3-dimensional mucosal culture model. Overall, trimethylation of histone that reportedly alters the chromatin structure is likely to contribute to the splicing pattern in the CLCA isoforms.
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Report
(5 results)
Research Products
(22 results)
