| Project/Area Number |
26670115
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
iijima norio 日本医科大学, 医学部, 准教授 (00285248)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | in vivoイメージング / 脳深部 / 光ファイバー / 無麻酔・非拘束 / in vioイメージング / ルシフェラーゼ / GFP / トランスジェニックラット / 光ファイバー束 / イメージング / ルシフェラーゼ発光 / アカルミネ / Per2::dLuc Tg ラット |
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| Outline of Final Research Achievements |
I was aiming in vivo imaging of rat deep brain in which luciferase was expressed in particular neurons using In Vivo Imaging System (IVIS) with Aka Lumine as a substrate of luciferase. Luciferase-expressing neurons were located in the hypothalamus (10 mm depth from the brain surface). However, no image of luciferase-expressing neurons was able to be captured even if from the top of removed brain. I needed to change experimental plan. I developed a new method for imaging of deep brain using bundle optical fibers (200 mm diameter, 3000 thin fibers were bundled). Clear images of GFP-expressing neurons in the deep brain were able to be captured. The bundle optical fiber was fixed with the cranium, which enable images of GFP-expressing neurons to be retained under free moving condition for several hours.
In addition, related to in vivo imaging, I was trying several kinds of experiments, for example anesthetic effect on luciferase-expression in the deep brain.
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