Development of in vivo imaging system from the deep brain using near infrared light.
| Project/Area Number |
26670115
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
iijima norio 日本医科大学, 医学部, 准教授 (00285248)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | in vivoイメージング / 脳深部 / 光ファイバー / 無麻酔・非拘束 / in vioイメージング / ルシフェラーゼ / GFP / トランスジェニックラット / 光ファイバー束 / イメージング / ルシフェラーゼ発光 / アカルミネ / Per2::dLuc Tg ラット |
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| Outline of Final Research Achievements |
I was aiming in vivo imaging of rat deep brain in which luciferase was expressed in particular neurons using In Vivo Imaging System (IVIS) with Aka Lumine as a substrate of luciferase. Luciferase-expressing neurons were located in the hypothalamus (10 mm depth from the brain surface). However, no image of luciferase-expressing neurons was able to be captured even if from the top of removed brain. I needed to change experimental plan. I developed a new method for imaging of deep brain using bundle optical fibers (200 mm diameter, 3000 thin fibers were bundled). Clear images of GFP-expressing neurons in the deep brain were able to be captured. The bundle optical fiber was fixed with the cranium, which enable images of GFP-expressing neurons to be retained under free moving condition for several hours.
In addition, related to in vivo imaging, I was trying several kinds of experiments, for example anesthetic effect on luciferase-expression in the deep brain.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Establishment of an in vitro cell line experimental system for the studablishment of an in vitro cell line experimental system for the study oy of inhalational anesthetic mechanisms.2016
Author(s)
Nagamoto S, Iijima N, Ishii H, Takumi K, Higo S, Aikawa S, Anzai M, Matsuo I, Nakagawa S, Takashima N, Shigeyoshi Y, Sakamoto A, Ozawa H.
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Journal Title
Neurosci Lett.
Volume: 4;620:
Pages: 163-168
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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