| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
To elucidate the function of calcium signaling in the mammary gland, we have analyzed the role of store-operated calcium entry in mammary epithelial cells using cell-type specific Stim1 or Stim2 or both knockout mice. We found that store-operated calcium entry is activated in mammary epithelial cells. We then established mice in which store-operated calcium entry is ablated in mammary epithelial cells. The mice normally grew up and no abnormality of the development of mammary gland, however, new-born pups nursed by female mice ablated both Stim1 and Stim2 in mammary epithelial cells showed severe growth retardation because of impaired secretion or production of milk. These results indicate that store-operated calcium entry is essential for the function of mammary epithelial cells.
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