Application of FALI with antibody-like scaffolds to the high-throughput functional proteomic screen for therapeutic targets
| Project/Area Number |
26670127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Naoto 埼玉大学, 理工学研究科, 教授 (60509727)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 神経科学 / プロテオーム / 組換え抗体 / 薬理学 |
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| Outline of Final Research Achievements |
Fluorophore-assisted light inactivation (FALI) in combination with a cell-based assay is a unique method for functional proteomic screen to search for regulators of a specific cellular function. One of the major obstacles for this method is the construction of antibody libraries containing high-affinity binders that cover cell surface membrane proteins as potential targets. To overcome barriers to efficiency, we tested new scaffolds compatible with cDNA display method. By using a single domain antibody framework derived from camel heavy-chain antibodies (VHH or nanobody), we optimized the cell-free expression of recombinant antibody clones that are linked to their genotype. Now we are trying pilot-scale clone selection for erbB as a model system.
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Report
(3 results)
Research Products
(5 results)
