Establishment of the strategy to purify transcription regulatory complex using immobilized DNA template

• Project/Area Number: 26670130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: Takahashi Hidehisa 北海道大学, 医学(系)研究科(研究院), 講師 (30423544)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 転写制御 / 発現制御 / 転写 / 転写因子 / プロテオミクス

Outline of Final Research Achievements

We performed the purification of transcription regulatory factors using immobilized promoter DNA template. We perform Mass spectromic analysis to
identify transcription regulatory factors. We took advantage of the method and found that novel transcription elongation complex called "little elongation complex (LEC)" is recruited to the snRNA gene promoters by Mediator complex.

Research Products

• [Int'l Joint Research] Stowers Institute for Medical Research(米国)
• [Journal Article] TRIM39 negatively regulates the NFκB-mediated signaling pathway through stabilization of Cactin (2016)
  • Author(s): Suzuki M, Watanabe M, Nakamaru Y, Takagi D, Takahashi H, Fukuda S, Hatakeyama S.
  • Journal Title: Cell Mol Life Sci. Volume: ７３ Issue: 5 Pages: 1085-1101
  • DOI: 10.1007/s00018-015-2040-x
  • NAID: 120005981342
  • Peer Reviewed / Open Access
• [Journal Article] Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes. (2016)
  • Author(s): Fujieda Y., Amengual O., Matsumoto M., Kuroki K., Takahashi H., Kono M., Kurita Y., Otomo K., Kato M., Oku K., Bohgaki T., Horita T., Yasuda S., Maenaka K., Hatakeyama S., Nakayama K.I. and Atsumi T. et al.
  • Journal Title: Rheumatology Volume: In press
  • Peer Reviewed
• [Journal Article] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex (2015)
  • Author(s): Takahashi, H., Takigawa, I., Watanabe, M., Anwar, D., Shibata, M., Tomomori-Sato, C., Sato, S., Ranjan, A., Seidel, C.W., Tsukiyama, T., Mizushima, W., Hayashi, M., Ohkawa, Y., Conaway, J.W., Conaway, R.C. and Hatakeyama, S
  • Journal Title: Nature Commun. Volume: 5 Issue: 1 Pages: 5941-5941
  • DOI: 10.1038/ncomms6941
  • NAID: 120005621881
  • Peer Reviewed / Open Access
• [Journal Article] Molecular role of RNF43 in canonical and noncanonical Wnt signaling (2015)
  • Author(s): T. Tsukiyama,et al.
  • Journal Title: Mol. Cell. Biol. Volume: in press Issue: 11 Pages: 2007-2023
  • DOI: 10.1128/mcb.00159-15
  • Peer Reviewed / Open Access
• [Journal Article] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipocyte activator PPAR gamma. (2015)
  • Author(s): Watanabe, M., Takahashi, H., Saeki, Y., Ozaki, T., Itoh, S., Suzuki, M., Mizushima, W., Tanaka, K. and Hatakeyama, S.
  • Journal Title: eLIFE Volume: 4
  • DOI: 10.7554/elife.05615
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TRIM29 regulates the p63-mediated pathway in cervical cancer cells. (2015)
  • Author(s): Masuda, Y., Takahashi, H. and Hatakeyama, S.
  • Journal Title: Biochim. Biophy. Acta-Mol. Cell Res Volume: 1853 Issue: 10 Pages: 2296-2305
  • DOI: 10.1016/j.bbamcr.2015.05.035
  • NAID: 120005906885
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin. (2015)
  • Author(s): Masuda, Y., Takahashi, H., Sato, S., Tomonori-Sato, C., Saraf, A., Washburn, M.P., Florens, L., Conaway, R.C., Conaway, J.W. and Hatakeyama, S.
  • Journal Title: Nature Commun. Volume: 6 Issue: 1 Pages: 7299-7299
  • DOI: 10.1038/ncomms8299
  • NAID: 120005647269
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2. (2015)
  • Author(s): Hayashi M, Maehara K, Harada A, Semba Y, Kudo K, Takahashi H, Oki S, Meno C, Ichiyanagi K, Akashi K, Ohkawa Y.
  • Journal Title: J Cell Biochem Volume: 117 Issue: 3 Pages: 780-792
  • DOI: 10.1002/jcb.25368
  • Peer Reviewed / Open Access
• [Journal Article] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ (2015)
  • Author(s): Watanabe M, Takahashi H, Saeki Y, Ozaki T, Itoh S, Suzuki M, Mizushima W, Tanaka K, Hatakeyama S
  • Journal Title: eLife Volume: In press
  • NAID: 120005617283
  • Peer Reviewed
• [Journal Article] Molecular role of RNF43 in canonical and noncanonical Wnt signaling (2015)
  • Author(s): Tsukiyama T, Fukui A, Terai S, Fujioka Y, Shinada K, Takahashi H, Terry P. Yamaguchi, Ohba Y, and Hatakeyama S
  • Journal Title: Molecular and cellular biology Volume: In press
  • NAID: 120005672365
  • Peer Reviewed
• [Presentation] Role of Human Mediator Subunit Med26 in Transcription Elongation (2015)
  • Author(s): Hidehisa Takahashi, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアホテル（兵庫県、神戸市）
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex (2015)
  • Author(s): Hidehisa Takahashi, Ichigaku Takigawa, Masashi Watanabe, Delnur Anwar, Mio Shibata, Chieri Tomomori-Sato, Shigeo Sato, Amol Ranjan, Chris W. Seidel, Tadasuke Tsukiyama, Wataru Mizushima, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama.
  • Organizer: Mechanism of Eukaryotic Transcription
  • Place of Presentation: Cold spring harbor laboratory（NY, USA）
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2015)
  • Author(s): 高橋秀尚, 畠山鎮次
  • Organizer: 第17回日本RNA学会年会
  • Place of Presentation: ホテルライフォート札幌（北海道札幌市中央区）
  • Year and Date: 2015-07-15
  • Invited
• [Presentation] メディエーター複合体による転写伸長制御 (2014)
  • Author(s): 高橋秀尚，瀧川一学，渡部昌，Delnur Anwar，柴田美音，佐藤チエリ，佐藤滋生，Amol Ranjan，Chris W. Seidel，築山忠維，林正康，大川恭行，Joan W. Conaway，Ronald C. Conaway，畠山 鎮次
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2014)
  • Author(s): 高橋秀尚，瀧川一学，渡部昌，Delnur Anwar，柴田美音，佐藤チエリ，佐藤滋生，Amol Ranjan，Chris Seidel，築山忠維，林正康，大川恭行，Joan W. Conaway，Ronald C. Conaway，畠山鎮次
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Remarks] 新規転写伸長制御因子Med26の機能解析
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/