Regulatory mechanisms of protein S-polythiolation
Project/Area Number |
26670131
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Kumamoto University |
Principal Investigator |
Sawa Tomohiro 熊本大学, 生命科学研究部, 教授 (30284756)
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Co-Investigator(Kenkyū-buntansha) |
IDA Tomoaki 東北大学, 大学院医学系研究科, 助教 (70570406)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | シグナル伝達 / プロテオミクス / 翻訳後修飾 / 酸化ストレス |
Outline of Final Research Achievements |
Protein S-polythiolation is a post-translational modification that introduce additional sulfur atoms on cysteine residues in proteins. We developed specific method that enables biotin-labeling on S-polythiolated cysteines by using methylsulfonyl benzothiazole and cyanide biotin. We successfully identified endogenous S-polythiolated proteins including cytoskeleton proteins. The method we developed may help further understanding of signal transduction mediated via protein S-polythiolation.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Persistent activation of cGMP-dependent protein kinase by a nitrated cyclic nucleotide via site specific protein S-guanylation2016
Author(s)
Akashi S, Ahmed KA, Sawa T, Ono K, Tsutsuki H, Burgoyne JR, Ida T, Horio E, Prysyazhna O, Oike Y, Rahaman MM, Eaton P, Fujii S, Akaike T.
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Journal Title
Biochemistry
Volume: 55
Issue: 5
Pages: 751-761
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] 8-Nitro-cGMP Enhances SNARE Complex Formation through S-Guanylation of Cys90 in SNAP252015
Author(s)
K. Kunieda, H. Tsutsuki, T. Ida, Y. Kishimoto, S. Kasamatsu, T. Sawa, N. Goshima, M. Itakura, M. Takahashi, T. Akaike, H. Ihara
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Journal Title
ACS Chemical Neuroscience
Volume: 6
Issue: 10
Pages: 1715-1725
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reactive sulfur species-mediated activation of the Keap1-Nrf2 pathway by 1,2-napthoquinone through sulfenic acids formation under oxidative stress.2015
Author(s)
Shinkai Y, Abiko Y, Ida T, Miura T, Kakehashi H, Ishii I, Nishida M, Sawa T, Akaike T, Kumagai Y.
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Journal Title
Chem Res Toxicol.
Volume: 印刷中
Issue: 5
Pages: 838-847
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling.2014
Author(s)
Ida T, Sawa T, Ihara H, Tsucr iya Y, Watanabe Y, Kumagai Y, Suematsu M, Motohashi H, Fujii S. Matsunaga T, Yamamoto M, Ono K, Devarie-Baez N O, Xian M, Fukuto J M, Akaike T.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: (印刷中)
Issue: 21
Pages: 7606-7611
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The redox chemistry and chemical biology of H2S, hydropersulfides and derivaed species: implications to their possible biological activity and utility.2014
Author(s)
Ono, K., Akaike T., Sawa, T., Kumagai, Y., Wink, D. A., Tantillo, D. J., Hobbs, A. J., Nagy, P., Xian, M., Lin, J., Fukuto, J.
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Journal Title
Free Radic. Biol. Med.
Volume: 77
Pages: 82-94
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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