Development of a method for quantification of phosphoinositides molecular species

• Project/Area Number: 26670132
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Akita University
• Principal Investigator: Sasaki Junko 秋田大学, 医学(系)研究科(研究院), 准教授 (30333371)
• Co-Investigator(Renkei-kenkyūsha): NAKANISHI HIROKI 秋田大学, 大学生体情報研究センター, 助教 (10466740)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 細胞内シグナル伝達 / イノシトールリン脂質

Outline of Final Research Achievements

Phosphoinositides (PIPs) are membrane lipids which play a role in various cellular functions. Recently a sensitive method for measuring PIPs molecular species is developed using mass spectrometry, however this method cannot discriminate among PIP1 and PIP2 isomers. We tried to establish a new method for measurement of these PIPs isomers.

Research Products

• [Journal Article] Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy. (2017)
  • Author(s): Kimura H, Eguchi S, Sasaki J, Kuba K, Nakanishi H, Takasuga S, Yamazaki M, Goto A, Watanabe H, Itoh H, Imai Y, Suzuki A, Mizushima N, Sasaki T
  • Journal Title: JCI Insight Volume: 2 Issue: 1 Pages: 89462-89478
  • DOI: 10.1172/jci.insight.89462
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Decreased 16:0/20:4-phosphatidylinositol level in the post-mortem prefrontal cortex of elderly patients with schizophrenia. (2017)
  • Author(s): Matsumoto J, Nakanishi H, Kunii Y, Sugiura Y, Yuki D, Wada A, Hino M, Niwa SI, Kondo T, Waki M, Hayasaka T, Masaki N, Akatsu H, Hashizume Y, Yamamoto S, Sato S, Sasaki T, Setou M, Yabe H.
  • Journal Title: Sci Rep. Volume: 7 Issue: 7 Pages: 45050-45050
  • DOI: 10.1038/srep45050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone deacetylase inhibitors inhibit metastasis by restoring a tumor suppressive microRNA-150 in advanced cutaneous T-cell lymphoma. (2017)
  • Author(s): Abe F, Kitadate A, Ikeda S, Yamashita J, Nakanishi H, Takahashi N, Asaka C, Teshima K, Miyagaki T, Sugaya M, Tagawa H.
  • Journal Title: Oncotarget Volume: 8 Issue: 5 Pages: 7572-7585
  • DOI: 10.18632/oncotarget.13810
  • Peer Reviewed / Open Access
• [Journal Article] Inositol Polyphosphate-4-Phosphatase Type I Negatively (2015)
  • Author(s): Nigorikawa, K., Hazeki, K., Sasaki, J., Omori, Y., Miyake, M., Morioka, S., Guo, Y., Sasaki, T., Hazeki, O
  • Journal Title: PLoS One Volume: 10 Issue: 11 Pages: 740-751
  • DOI: 10.1371/journal.pone.0142091
  • Peer Reviewed / Open Access
• [Journal Article] INPP4B is a PtdIns(3,4,5)P3 phosphatase that can act as a tumor suppressor. (2015)
  • Author(s): Kofuji S, Kimura H, Nakanishi H, Nanjo H, Takasuga S, Liu H, Eguchi S, Nakamura R, Itoh R, Ueno N, Asanuma K, Huang M, Koizumi A, Habuchi T, Yamazaki M, Suzuki A, Sasaki J, Sasaki T.
  • Journal Title: Cancer Discovery Volume: in press
• [Journal Article] In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes. (2015)
  • Author(s): Chew CL, Lunardi A, Gulluni F, Ruan DT, Chen M, Salmena L, Nishino M, Papa A, Ng C, Fung J, Clohessy JG, Sasaki J, Sasaki T, Bronson RT, Hirsch E, Pandolfi PP
  • Journal Title: Cancer Discovery Volume: in press
• [Journal Article] Loss of INPP4B causes a DNA repair defect through loss of BRCA1, ATM and ATR and can be targeted with PARP inhibitor treatment. Oncotarget. (2015)
  • Author(s): Ip LR, Poulogiannis G, Viciano FC, Sasaki J, Kofuji S, Spanswick VJ, Hochhauser D, Hartley JA, Sasaki T, Gewinner CA.
  • Journal Title: Oncotarget Volume: in press
• [Journal Article] Dachsous-Dependent Asymmetric Localization of Spiny-Legs Determines Planar Cell Polarity Orientation in Drosophila. (2014)
  • Author(s): Ayukawa T., Akiyama M., Mummery-Widmer JL., Stoeger T., Sasaki J., Knoblich JA., Senoo H., Sasaki T., Yamazaki M.
  • Journal Title: Cell Rep. Volume: 8 Issue: 2 Pages: 610-621
  • DOI: 10.1016/j.celrep.2014.06.009
  • Peer Reviewed / Open Access
• [Journal Article] Phosphatidic acid-preferring phospholipase A1 regulates mitochondrial dynamics. (2014)
  • Author(s): Baba T, Kashiwagi Y, Arimitsu N, Kogure T, Edo A, Maruyama T, Nakao K, Nakanishi H, Kinoshita M, Frohman MA, Yamamoto A, Tani K.
  • Journal Title: Journal of Biological Chemistry Volume: 289 Issue: 16 Pages: 11497-11511
  • DOI: 10.1074/jbc.m113.531921
  • Peer Reviewed / Open Access
• [Journal Article] Very-long-chain polyunsaturated fatty acids accumulate in phosphatidylcholine of fibroblasts from patients with Zellweger syndrome and acyl-CoA oxidase1 deficiency (2014)
  • Author(s): Yuichi Abe, Masanori Honsho, Hiroki Nakanishi, Ryo Taguchi, Yukio Fujiki
  • Journal Title: BBA - Molecular and Cell Biology of Lipids Volume: 1841 Issue: 4 Pages: 610-619
  • DOI: 10.1016/j.bbalip.2014.01.001
  • Peer Reviewed / Open Access
• [Journal Article] リピドミクスの分析技術と将来展望 (2014)
  • Author(s): 中西広樹
  • Journal Title: オレオサイエンス誌 Volume: 8 Pages: 321-327
  • NAID: 130005308258
• [Presentation] A method for studying quality of phosphoinositides (2016)
  • Author(s): Takehiko Sasaki, Hiroki Nakanishi, Satoshi Eguchi, Masaki Ishikawa, Akira Suzuki, Junko Sasaki
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] ホスホイノシタイドの新しい解析方法 (2015)
  • Author(s): 中西広樹、江口賢史、石川将己、鈴木聡、佐々木純子、佐々木雄彦
  • Organizer: 第38回日本分子生物学会年会・第88回生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 心肥大におけるホスホイノシタイド代謝酵素Vps34のタンパク質分解機構の役割 (2015)
  • Author(s): 木村洋貴、江口賢史、久場敬司、今井由美子、高須賀俊輔、伊藤玲悦、中村亮太郎、中西広樹、石川将己、佐々木純子、山崎正和、佐々木雄彦
  • Organizer: 第38回日本分子生物学会年会・第88回生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] イノシトールリン脂質研究の新しい方法論の提示 (2015)
  • Author(s): 中西広樹、小藤智史、江口賢史、中村亮太郎、石川将己、高須賀俊輔、佐々木純子、佐々木雄彦
  • Organizer: 第14回生命科学研究会
  • Place of Presentation: 三浦市民ホール
  • Year and Date: 2015-06-26
• [Presentation] The class III PI3K and phosphatidylinositol 3-phosphate ensure structural and functional integrity of cardiomyocytes (2015)
  • Author(s): Hirotaka Kimura, Satoshi Eguchi, Junko Sasaki, Takehiko Sasaki
  • Organizer: the 120th Annual Meeting of The Japanese Association of Anatomists, the 92nd Annual Meeting of The Physiological Society of Japan
  • Place of Presentation: 兵庫
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The novel mechanism of tumor suppression by INPP4B (2015)
  • Author(s): Junko Sasaki, Satoshi Kofuji, Takahiro Kimura, Shunsuke Takasuga, Hiroki Nakanishi, Takehiko Sasaki
  • Organizer: 2nd International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling
  • Place of Presentation: 東京
  • Year and Date: 2015-01-23 – 2015-01-24
• [Book] 生体の科学 (2016)
  • Author(s): 中西広樹
  • Total Pages: 5
  • Publisher: 医学書院
• [Remarks]
  • URL: http://www.med.akita-u.ac.jp/~bisei/
• [Patent(Industrial Property Rights)] ホスホイノシタイド分離測定法の開発. (2017)
  • Inventor(s): 中西広樹 佐々木雄彦 佐々木純子 江口賢史 中西貴代
  • Industrial Property Rights Holder: 秋田大学 ALTe
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-051354
  • Filing Date: 2017-03-16
• [Patent(Industrial Property Rights)] 新規リン脂質およびその利用. (2016)
  • Inventor(s): 佐々木雄彦 中西広樹 石川将己 上野紀子 江口賢史 佐々木純子
  • Industrial Property Rights Holder: 秋田大学 ALTe
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-144177
  • Filing Date: 2016-07-22