Study on regulation of addipocyte differentiation through the control of mRNA splicing activity

Research Project

Project/Area Number	26670134
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	General medical chemistry
Research Institution	University of Toyama
Principal Investigator	Kaida Daisuke 富山大学, 先端ライフサイエンス拠点, 准教授 (60415122)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	スプライシング / snRNP
Outline of Final Research Achievements	We treated mouse 3T3-L1 cells with a splicing inhibitor that binds and inhibits U2 snRNP, and found that adipogenesis was inhibited in a dose dependent manner. The significant inhibition of adipogenesis was observed if the cells were treated with the splicing inhibitor when the cells were cultured in differentiation medium. We also found that some introns showed resistance against splicing inhibition in a intron- and snRNA-dependent manner. These dependency might affect differentiation efficiency.

Report

(3 results)