Research Project
Grant-in-Aid for Challenging Exploratory Research
In the first year of the project, we have established MEF-/- cells (MEF cells derived from NRDc-deficient mice) stably re-introduced with wild-type or enzymatically inactive mutant of NRDc. We also established monoclonal antibody against NRDc, which functions well for chromatin immunoprecipitation (ChIP). In the second year, by using those materials, we performed ChIP sequence and RNA sequence to globally identify the target genes for NRDc. As a result, we identified some hundreds of target genes, some of which were differentially regulated by the enzymatic activity of NRDc. We have also confirmed that the level of inflammation in three mouse models of inflammatory diseases (inflammatory bowel disease, NASH and rheumatoid arthritis) is significantly lower in NRDc-deficient mice compared with control mice.
All 2015 2014
All Journal Article (2 results) (of which Peer Reviewed: 2 results, Open Access: 2 results, Acknowledgement Compliant: 2 results) Presentation (21 results) (of which Int'l Joint Research: 1 results, Invited: 3 results)
Plos One
Volume: 9 Issue: 5 Pages: e98017-e98017
10.1371/journal.pone.0098017
Sci Rep.
Volume: 4 Issue: 1 Pages: 5094-5094
10.1038/srep05094
