| Project/Area Number |
26670143
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NANBA DAISUKE 東京医科歯科大学, 難治疾患研究所, 准教授 (10380255)
FUKUDA SHINJI 愛媛大学, プロテオサイエンスセンター, 講師 (70398238)
NAKAYAMA HIROINAO 愛媛大学, プロテオサイエンスセンター, 助教 (40512132)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エクトドメイン・シェディング / がん細胞不均一性 / proHB-EGF / ゆらぎ / 細胞形質転換 |
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| Outline of Final Research Achievements |
We analyzed the relation between phenotypic transition of tumor cells and ectodomain shedding activity of membrane-anchored growth factor proHB-EGF in breast tumor MCF-7 cells. We established two MCF-7 clones and further analyzed. One (clone #8) mostly generated epithelial-like colonies, while the other (clone #5) produced both epithelial- and fibroblast-like colonies. We demonstrated that proHB-EGF was more shed in breast tumor MCF-7 cells with epithelial-like morphology than those with fibroblast-like morphology. We speculate that ectodomain shedding of proHB-EGF could be associated with morphological phenotype transition of breast tumor cells.
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