| Project/Area Number |
26670144
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
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Principal Investigator |
Numaga-Tomita Takuro 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (60705060)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | メカノバイオロジー / 心血管細胞 / 血管成熟 / 細胞外マトリクス |
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| Outline of Final Research Achievements |
Cardiovascular tissue is physically dynamic. Therefore, most of cardiovascular cells maintain their homeostasis by adopting to physical changes of extracellular environment.However underlying mechanisms remain elusive. In this study, we focused on microenvironmental stiffness surrounding cardiovascular cells and studied how the cells respond and adapt to the changes of extracellular stiffness. At first we hod tried to utilize hydrogel which change their elasticity in response to light stimulation. However, the hydrogel was instable and difficult to manipulate. Therefore, we developed the culture system based on PDMS with different stiffness and revealed a possibility that the stiffness close to healthy tissue was critical to supported stress reduction by secretion.
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