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In vitro induction of meiosis from embryonic stem cells and germ line stem cells

Research Project

Project/Area Number 26670162
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pathological medical chemistry
Research InstitutionSaitama Medical University

Principal Investigator

Suzuki Ayumu  埼玉医科大学, 医学部, 助教 (80639708)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords減数分裂 / Max / ES細胞 / 生殖細胞 / 精原幹細胞
Outline of Final Research Achievements

Germ cell is the only cell-type that can transfer genetic and epigenetic information to subsequent generations. Although germ cells are, like somatic cells, able to increase their numbers by mitosis, germ cells stop undergoing mitosis and instead initiate meiosis to convert them to haploid cells at appropriate time points in which female germ cells onset meiosis in the genital ridge at midgestation, while male germ cells initiate this switch after birth in the testes of mammals. Although retinoic acid is known to be crucially involved in this meiotic onset, the molecular mechanisms governing the onset of this special cell division remain largely obscure. However, as an outcome of this research project, I could identify MAX as a negative regulator of meiosis, suppressing ectopic and precocious meiotic onset in embryonic stem cells (ESCs) and spermatogonial stem cells (SSCs), respectively. Thus, I could provide a novel strategy for studying the molecular bases of meiosis.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (2 results)

  • [Int'l Joint Research] Salk Institute(米国vvv)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Max as a biological blockade that restricts meiotic process in ESCs.2016

    • Author(s)
      Suzuki A, Hirasaki M, Hishida T, Okamura D, Wu J, Ueda A, Nishimoto M, Nakachi Y, Mizuno Y, Okazaki Y, Matsui Y, *Izpisua Belmonte JC, Okuda A.
    • Journal Title

      Nat Commun

      Volume: 7 Issue: 1 Pages: 11056-11056

    • DOI

      10.1038/ncomms11056

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Forced expression of Nanog or Esrrb preserves the ESC status in the absence of nucleostemin expression2015

    • Author(s)
      Katano M, Ema M, Nakachi Y, Mizuno Y, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Takahashi S, Okazaki, Okuda A.
    • Journal Title

      STEM CELLS

      Volume: 33 Issue: 4 Pages: 1089-1101

    • DOI

      10.1002/stem.1918

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional crosstalk between Myc and PI3K signaling for supporting unlimited self-renewal of embryonic stem cells2015

    • Author(s)
      Hishida T, Nakachi Y, Mizuno Y, Katano M, Okazaki Y, Ema M, Takahashi S, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Hishida-Nozaki Y, Vazquez-Ferrer E, Sancho-Martinez I, Izpisua Belmonte JC, Okuda A.
    • Journal Title

      STEM CELLS

      Volume: 33 Issue: 3 Pages: 713-725

    • DOI

      10.1002/stem.1893

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells2014

    • Author(s)
      Kamon M, Katano M, Hiraki K, Hishida T, Nakachi Y, Mizuno Y, Okazaki Y, Suzuki A, Hirasaki M, Ueda A, Nishimoto M, Kato H, Okuda A.
    • Journal Title

      Stem Cells and Development

      Volume: 23 Issue: 18 Pages: 2170-2179

    • DOI

      10.1089/scd.2013.0326

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] マウスES細胞における減数分裂抑制機構の発見2015

    • Author(s)
      鈴木 歩 , 平崎 正孝 , 上田 篤 , 松居 靖久 , 奥田 晶彦
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸ポートアイランド兵庫県神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウスES細胞においてNucleosteminノックアウトによる未分化性の消失はNanogもしくはEsrrbタンパク質強制発現により回避される2014

    • Author(s)
      片野 幸、水野洋介、仲地 豊、平崎正孝、鈴木 歩、西本正純、岡崎康司、奥田晶彦
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-01-27  

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