In vitro induction of meiosis from embryonic stem cells and germ line stem cells
| Project/Area Number |
26670162
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Saitama Medical University |
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Principal Investigator |
Suzuki Ayumu 埼玉医科大学, 医学部, 助教 (80639708)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 減数分裂 / Max / ES細胞 / 生殖細胞 / 精原幹細胞 |
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| Outline of Final Research Achievements |
Germ cell is the only cell-type that can transfer genetic and epigenetic information to subsequent generations. Although germ cells are, like somatic cells, able to increase their numbers by mitosis, germ cells stop undergoing mitosis and instead initiate meiosis to convert them to haploid cells at appropriate time points in which female germ cells onset meiosis in the genital ridge at midgestation, while male germ cells initiate this switch after birth in the testes of mammals. Although retinoic acid is known to be crucially involved in this meiotic onset, the molecular mechanisms governing the onset of this special cell division remain largely obscure. However, as an outcome of this research project, I could identify MAX as a negative regulator of meiosis, suppressing ectopic and precocious meiotic onset in embryonic stem cells (ESCs) and spermatogonial stem cells (SSCs), respectively. Thus, I could provide a novel strategy for studying the molecular bases of meiosis.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Max as a biological blockade that restricts meiotic process in ESCs.2016
Author(s)
Suzuki A, Hirasaki M, Hishida T, Okamura D, Wu J, Ueda A, Nishimoto M, Nakachi Y, Mizuno Y, Okazaki Y, Matsui Y, *Izpisua Belmonte JC, Okuda A.
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Journal Title
Nat Commun
Volume: 7
Issue: 1
Pages: 11056-11056
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Forced expression of Nanog or Esrrb preserves the ESC status in the absence of nucleostemin expression2015
Author(s)
Katano M, Ema M, Nakachi Y, Mizuno Y, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Takahashi S, Okazaki, Okuda A.
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Journal Title
STEM CELLS
Volume: 33
Issue: 4
Pages: 1089-1101
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Functional crosstalk between Myc and PI3K signaling for supporting unlimited self-renewal of embryonic stem cells2015
Author(s)
Hishida T, Nakachi Y, Mizuno Y, Katano M, Okazaki Y, Ema M, Takahashi S, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Hishida-Nozaki Y, Vazquez-Ferrer E, Sancho-Martinez I, Izpisua Belmonte JC, Okuda A.
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Journal Title
STEM CELLS
Volume: 33
Issue: 3
Pages: 713-725
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells2014
Author(s)
Kamon M, Katano M, Hiraki K, Hishida T, Nakachi Y, Mizuno Y, Okazaki Y, Suzuki A, Hirasaki M, Ueda A, Nishimoto M, Kato H, Okuda A.
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Journal Title
Stem Cells and Development
Volume: 23
Issue: 18
Pages: 2170-2179
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] マウスES細胞における減数分裂抑制機構の発見2015
Author(s)
鈴木 歩 , 平崎 正孝 , 上田 篤 , 松居 靖久 , 奥田 晶彦
Organizer
BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
Place of Presentation
神戸ポートアイランド兵庫県神戸市
Year and Date
2015-12-01
Related Report
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