Mass spectrometry analysis of cardiac tissue with acute infarction
| Project/Area Number |
26670173
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
羽賀 博典 京都大学, 医学研究科, 教授 (10252462)
宮川 文 京都大学, 医学研究科, 特定准教授 (90432385)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 急性心筋梗塞 / 質量分析 / 病理組織 / 心筋梗塞 / イメージング / バイオマーカー |
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| Outline of Final Research Achievements |
It had remained difficult to make a pathologic diagnosis of acute myocardial infarction (AMI) at the very early phase, because histological alterations are rarely observed. We developed a novel pre-treatment method of the cardiac tissue and used an imaging mass spectrometry (IMS) system to obtain multiple mass spectra for specific cardiac peptides at a wide m/z range. IMS data demonstrated alteration of signals of the mitochondrial adenosine triphosphate synthase alpha subunit and other cardiac proteins in the infarcted cardiac tissue. In conclusion, we were successful in the direct identification of molecular changes in the cardiac tissue. IMS is a promising technique for the identification of biomarkers for AMI and other pathological studies.
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Report
(4 results)
Research Products
(6 results)
