Role of Non-coding RNA of T-UCR in exosome in diagnosis and therapy of gastric and prostatic cancer

• Project/Area Number: 26670175
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Hiroshima University
• Principal Investigator: YASUI WATARU 広島大学, 医歯薬保健学研究院, 教授 (40191118)
• Co-Investigator(Renkei-kenkyūsha): OUE NAOHIDE 広島大学, 大学院医歯薬保健学研究院, 准教授 (60346484)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エクソソーム / ncRNA / 超保存領域（T-UCR） / 胃癌 / 前立腺癌 / 診断・治療展開

Outline of Final Research Achievements

By the analysis of Transcribed ultraconserved region (T-UCR) transcripts, the levels of many T-UCRs are found to be altered in cancers. Among those, Uc.416+A,up-regulated in cancer, was negatively regulated by miR-153 and participated in cell growth through IGFBP6 and HOXB5/B6. Uc.416+A was secreted from cancer cells and high levels were detected in the sera of cancer patients. Application to liquid biopsy system is expected.

Research Products

• [Journal Article] The transcribed-ultra conserved regions in prostate and gastric cancer: DNA hypermethylation and microRNA-associated regulation. (2015)
  • Author(s): Keisuke Goto, Sho Ishikawa,Ririno Honma, Keiji Tanimoto, Naoya Sakamoto,Kazuhiro Sentani, Naohide Oue, Jun Teishima, Akio Matsubara and Wataru Yasui.
  • Journal Title: Oncogene Volume: 1 Issue: 27 Pages: 1-1
  • DOI: 10.1038/onc.2015.445
  • Peer Reviewed / Open Access
• [Journal Article] Characteristic miR-24 Expression in Gastric Cancers among Atomic Bomb Survivors. (2015)
  • Author(s): Naito Y, Oue N, Pham TT, Yamamoto M, Fujihara M, Ishida T, Mukai S, Sentani K, Sakamoto N, Hida E, Sasaki H, Yasui W.
  • Journal Title: Pathobiology Volume: 82 Issue: 2 Pages: 68-75
  • DOI: 10.1159/000398809
  • Peer Reviewed
• [Journal Article] Significance of miR-148a in Colorectal Neoplasia: Downregulation of miR-148a Contributes to the Carcinogenesis and Cell Invasion of Colorectal Cancer. (2015)
  • Author(s): Hibino Y, Sakamoto N, Naito Y, Goto K, Oo HZ, Sentani K, Hinoi T, Ohdan H, Oue N, Yasui W.
  • Journal Title: Pathobiology Volume: 82 Issue: 5 Pages: 233-241
  • DOI: 10.1159/000438826
  • Peer Reviewed
• [Journal Article] Tetraspanin 8, identified by the Escherichia coli ampicillin secretion trap, is associated with cell growth and invasion in gastric cancer (2015)
  • Author(s): Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Naito Y, Oo HZ, Yasui W
  • Journal Title: Gastric Cancer Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High miR-21 expression from FFPE tissues is associated with poor survival and response to adjuvant chemotherapy in colon cancer. (2014)
  • Author(s): Oue N, Anami K, Schetter AJ, Moehler M, Okayama H, Khan MA, Bowman ED, Mueller A, Schad A, Shimomura M, Hinoi T, Aoyagi K, Sasaki H, Okajima M, Ohdan H, Galle PR, Yasui W, Harris CC
  • Journal Title: Int J Cancer Volume: 134 Issue: 8 Pages: 1926-1934
  • DOI: 10.1002/ijc.28522
  • Peer Reviewed / Open Access
• [Journal Article] MiR-148a is down-regulated in gastric cancer, targets MMP7 and indicates tumor invasiveness and poor prognosis (2014)
  • Author(s): Naoya Sakamoto, Yutaka Naito, Naohide Oue, Kazuhiro Sentani, Naohiro Uraoka, Htoo Zarni Oo, Kazuyoshi Yanagihara, Kazuhiko Aoyagi, Hiroki Sasaki, Wataru Yasui
  • Journal Title: Cancer Science Volume: 105 Issue: 2 Pages: 236-243
  • DOI: 10.1111/cas.12330
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA-143 regulates collagen type III expression in stromal fibroblasts of scirrhous typoe gastric cancer (2014)
  • Author(s): Yutaka Naito, Naoya Sakamoto, Naohide Oue, Masakazu Yashiro, Kazuhiro Sentani, Kazuyoshi Yanagihara, Kosei Hirakawa, Wataru Yasui
  • Journal Title: Cancer Science Volume: 105 Issue: 2 Pages: 228-235
  • DOI: 10.1111/cas.12329
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA-145 is a potential prognostic factor of scirrhous type gastric cancer (2014)
  • Author(s): Yutaka Naito, Kyohei Yasuno, Hiroko Tagawa, Naoya Sakamoto, Naohide Oue, Masakazu Yashiro, Kazuhiro Sentani, Keisuke Goto, Shunsuke Shinmei, Htoo Zarni Oo, Kazuyoshi Yanagihara, Kosei Hirakawa, Wataru Yasui
  • Journal Title: Oncology Reports Volume: 32 Issue: 4 Pages: 1720-1726
  • DOI: 10.3892/or.2014.3333
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] スキルス胃がん微小環境における細胞外小胞の機能 (2015)
  • Author(s): 内藤 寛、八代正和、平川弘聖、安井 弥、落合孝広
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Molecular pathology of gastric cancer (2015)
  • Author(s): Yasui W
  • Organizer: The 2nd International Forum for Japan and Korea Medical Exchange
  • Place of Presentation: Kure, Japan
  • Year and Date: 2015-10-17
  • Int'l Joint Research / Invited
• [Presentation] スキルス胃癌微小環境における細胞外小胞の機能解析 (2015)
  • Author(s): 内藤 寛、八代正和、平川弘聖、安井 弥、落谷孝広
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] 転写超保存領域T-UCRの胃癌・前立腺癌における発現制御機構 (2015)
  • Author(s): 後藤景介、石川 聖、本間りりの、坂本直也、仙谷和弘、大上直秀、亭島 淳、松原昭郎、安井 弥
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] スキルス胃癌微小環境における分泌型microRNAの機能解析 (2015)
  • Author(s): 内藤 寛、八代正和、平川弘聖、安井 弥、落合孝広
  • Organizer: 第7回日本RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 2015-08-26
• [Presentation] Molecular pathology of gastric cancer (2015)
  • Author(s): Yasui W
  • Organizer: JP Kim Memorial International Gastric Cancer Symposium July 31, 2015
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2015-07-31
  • Int'l Joint Research / Invited
• [Presentation] Endoscopic management of early gastric cancer: Molecular analysis in endoscopic specimen (2015)
  • Author(s): Yasui W
  • Organizer: The 11th International Gastric Cancer Congress
  • Place of Presentation: Sao Paulo, Brazil
  • Year and Date: 2015-06-04
  • Int'l Joint Research / Invited
• [Presentation] 非翻訳領域をコードする転写超保存領域T-UCRの胃癌における発現、機能解析 (2015)
  • Author(s): 後藤景介、石川 聖、仙谷和弘、大上直秀、亭島 淳、松原昭郎、安井 弥
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30
• [Presentation] 前立腺癌における転写超保存領域T-UCRの発現調節機構 (2015)
  • Author(s): 関野陽平、後藤景介、石川 聖、仙谷和弘、大上直秀、亭島 淳、松原昭郎、安井 弥
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30
• [Presentation] Functional analysis of extracellular vesicles as a novel regulatory agent of scirrhous type gastric cancer microenvironment. (2015)
  • Author(s): Naito Y, Yashiro M, Hirakawa K, Yasui W and Ochiya T
  • Organizer: International Society for Extracellular Vesicles 2015
  • Place of Presentation: Washington D.C. (USA),
  • Year and Date: 2015-04-23
  • Int'l Joint Research
• [Presentation] Molecular pathology of cancer cell growth and progression in gastrointestinal cancer International Symposium on Tissue Microenvironmental Modulators (2015)
  • Author(s): Yasui W
  • Organizer: International Symposium on Tissue Microenvironmental Modulators
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-03-14
  • Invited
• [Presentation] Novel biomarkers and therapeutic targets of gastrointestinal cancer identified through transcriptome dissection (2015)
  • Author(s): Yasui W
  • Organizer: The 2nd International Symposium of Hiroshima Research Center for Healthy Ageing
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2015-03-13
  • Invited
• [Presentation] 世界の胃癌診療における日本の病理の役割：過去・現在・未来 (2015)
  • Author(s): 安井 弥
  • Organizer: 第87回日本胃癌学会総会
  • Place of Presentation: 広島
  • Year and Date: 2015-03-04 – 2015-03-06
  • Invited
• [Presentation] トランスクリプトームダイセクションによって同定した胃がんの新規診断・治療標的 (2014)
  • Author(s): 安井 弥、大上直秀、Htoo Zarni Oo、向井正一朗、仙谷和弘
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Presentation] 非翻訳RNAコードする超保存領域の胃癌における発現解析 (2014)
  • Author(s): 後藤景介、石川 聖、服部拓也、浦岡直礼、仙谷和弘、大上直秀、亭島 淳、松原昭郎、安井 弥
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 転写超保存領域T－UCRの前立腺癌における発現解析 (2014)
  • Author(s): 後藤景介、石川 聖、内藤 寛、Oo Htoo Zarni、坂本直也、仙谷和弘、大上直秀、亭島 淳、松原昭郎、安井 弥
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 胃癌における細胞表面蛋白質TSPAN8の発現とその意義 (2014)
  • Author(s): 服部拓也、大上直秀、仙谷和弘、Htoo Zarni Oo、阿南勝宏、安井 弥
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] スキルス胃癌間質の線維芽細胞におけるmicroRNA－145の機能 (2014)
  • Author(s): 田川寛子、内藤 寛、安野恭平、坂本直也、仙谷和弘、大上直秀、安井 弥
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 胃癌における非翻訳領域をコードする転写超保存領域の発現調節機構 (2014)
  • Author(s): 石川 聖、後藤景介、内藤 寛、Oo Htoo Zarni、坂本直也、仙谷和弘、大上直秀、安井 弥
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Remarks] 広島大学大学院分子病理学研究室ホームページ
  • URL: http://home.hiroshima-u.ac.jp/byori1/
• [Remarks] 広島大学大学院 医歯薬保健学研究院 分子病理学研究室 ホームページ
  • URL: http://home.hiroshima-u.ac.jp/byori1/