Development of histo-in situ technique demonstrating sequencing specific methyalted cytosine
| Project/Area Number |
26670176
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
北澤 理子 愛媛大学, 医学部附属病院, 准教授 (00273780)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | DNAメチル化 / in situ hybridization / Histochemistry / Epigenetics / 錯体形成 / 組織化学 / エピジェネティク / メチル化シトシン |
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| Outline of Final Research Achievements |
By making a ICON a probe which specifically cross-link and bound to the methylated cytosine, probe DNA can specifically hybridize to the target DNA in a sequence dependent manner. By PRINS method, a DNA elongation reaction was established using bounded ICON probe on the specific ch romosome. Modified nucleotides were incorporated during this elongation stem and visualized by a conventio nal histochemical techniques. By this easy, methyalation status of specific chromosome or DNA sequence cou Id be visualized. So far we gathered a lot of basic and fundamental data. \Ve are now currently trying to v isualize a single-copy gene during spermatogenesis in mouse testis, and updating our protocol for more sta ble and reliable one.
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Report
(4 results)
Research Products
(12 results)
