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Development of histo-in situ technique demonstrating sequencing specific methyalted cytosine

Research Project

Project/Area Number 26670176
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Human pathology
Research InstitutionEhime University

Principal Investigator

KITAZAWA SOHEI  愛媛大学, 医学系研究科, 教授 (90186239)

Co-Investigator(Kenkyū-buntansha) 北澤 理子  愛媛大学, 医学部附属病院, 准教授 (00273780)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsDNAメチル化 / in situ hybridization / Histochemistry / Epigenetics / 錯体形成 / 組織化学 / エピジェネティク / メチル化シトシン
Outline of Final Research Achievements

By making a ICON a probe which specifically cross-link and bound to the methylated cytosine, probe DNA can specifically hybridize to the target DNA in a sequence dependent manner. By PRINS method, a DNA elongation reaction was established using bounded ICON probe on the specific ch romosome. Modified nucleotides were incorporated during this elongation stem and visualized by a conventio nal histochemical techniques. By this easy, methyalation status of specific chromosome or DNA sequence cou Id be visualized. So far we gathered a lot of basic and fundamental data. \Ve are now currently trying to v isualize a single-copy gene during spermatogenesis in mouse testis, and updating our protocol for more sta ble and reliable one.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 8 results,  Acknowledgement Compliant: 5 results) Presentation (3 results)

  • [Journal Article] TDRG1 functions in testicular seminoma are dependent on the PI3K/Akt/mTOR signaling pathway2016

    • Author(s)
      Yong Wang, Yu Gan, Zhengyu Tan, Jun Zhou, Riko Kitazawa, Xianzhen Jiang, Yuxin Tang, and Jianfu Yang
    • Journal Title

      OncoTargets and Therapy

      Volume: 7 Issue: 7 Pages: 409-420

    • DOI

      10.1080/15384047.2016.1178425

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phospholipase Cε activates nuclear factor-κB signaling by causing cytoplasmic localization of ribosomal S6 kinase and facilitating its phosphorylation of inhibitor κB in colon epithelial cells.2016

    • Author(s)
      Masahiro Wakita, Hironori Edamatsu, Mingzhen Li, Aki Emi, Sohei Kitazawa, Tohru Kataoka
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 291巻 Issue: 24 Pages: 12586-12600

    • DOI

      10.1074/jbc.m116.717561

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] sFRP4-dependent Wnt signal modulation is critical for bone remodeling during postnatal development and age-related bone loss.2016

    • Author(s)
      Haraguchi R, Kitazawa R, Mori K, Tachibana R, Kiyonari H, Imai Y, Abe T, Kitazawa S.
    • Journal Title

      Sci Rep.

      Volume: 6:25198 Issue: 1 Pages: 1-14

    • DOI

      10.1038/srep25198

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Epigenetic regulation of Tbx18 gene expression during endochondral bone formation.2015

    • Author(s)
      Haraguchi R, Kitazawa R, Kitazawa S
    • Journal Title

      Cell Tissue Res

      Volume: 359 Issue: 2 Pages: 503-512

    • DOI

      10.1007/s00441-014-2028-0

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reactivation of CDX2 in Gastric Cancer as Mark for Gene Silencing Memory2015

    • Author(s)
      Kameoka Y, Kitazawa R, Ariasu K, Tachibana R, Mizuno Y, Haraguchi R, Kitazawa S.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 48 Issue: 4 Pages: 115-124

    • DOI

      10.1267/ahc.15014

    • NAID

      130005095243

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] BOB.1-positive Classical Hodgkin’s Lymphoma Carries Hypermethylation of Its Promoter as Epigenetic Marker of Gene-silencing Memory2014

    • Author(s)
      Watanabe T, Kitazawa R, Mizuno Y, Kuwahara N, Ito C, Sugita A, Haraguchi R, Kitazawa S
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 47 Issue: 3 Pages: 125-131

    • DOI

      10.1267/ahc.14012

    • NAID

      130004137836

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diabetic osteopenia by decreased β-catenin signaling is partly induced by epigenetic derepression of sFRP-4 gene2014

    • Author(s)
      Mori K, Kitazawa R, Kondo T, Mori M, Hamada Y, Nishida M, Minami Y, Haraguchi R, Takahashi Y, Kitazawa S.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 7 Pages: e102797-e102797

    • DOI

      10.1371/journal.pone.0102797

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Duodenal gastric heterotopia, sporadic or fundic gland polyp-associated, frequently carries β-catenin mutation.2014

    • Author(s)
      Nakagawa M, Kitazawa R, Kondo T, Ninomiya K, Okita M, Haraguchi R, Kitazawa S
    • Journal Title

      Virchows Arch

      Volume: 465 Issue: 3 Pages: 253-256

    • DOI

      10.1007/s00428-014-1612-8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Acquisition of MYD88 L265P mutation during treatment of diffuse large B-cell lymphoma of parotid gland.2014

    • Author(s)
      Koto Fujiishii, Riko Kitazawa, Yusa Nagai, Takafumi Watanabe, Kenji Bando, Shinji Kobayashi, Yoshihiro Yakushijin, Ryuma Haraguchi, Sohei Kitazawa.
    • Journal Title

      Virchows Archiv

      Volume: 464(1) Issue: 1 Pages: 121-124

    • DOI

      10.1007/s00428-013-1514-1

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 破骨細胞分化因子受容体RANKの新規変異体vRANKの解析2014

    • Author(s)
      北澤理子, 向井智美, 永井由紗, 原口竜摩, 北澤荘平
    • Organizer
      日本病理学会
    • Place of Presentation
      広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト副甲状腺二次性過形成組織におけるPadlock probeを用いた組織切片上でのメチル化検出2014

    • Author(s)
      二宮 鴻介, 北澤 荘平, 沖田 将慶, 菊澤 里佳子, 伊藤 才季, 原口 竜摩, 北澤 理子
    • Organizer
      日本病理学会
    • Place of Presentation
      広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 単相性と二相性の滑膜肉腫における発現遺伝子の違い2014

    • Author(s)
      水野洋輔, 北澤理子, 杉田敦郎, 久野美子, 原口竜摩, 北澤荘平
    • Organizer
      日本病理学会
    • Place of Presentation
      広島
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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