| Project/Area Number |
26670182
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | National Center for Geriatrics and Gerontology |
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Principal Investigator |
TAKESHITA SUNAO 国立研究開発法人国立長寿医療研究センター, 国立長寿医療研究センター, 研究室長 (50263009)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨代謝 / 骨吸収 / 骨基質 / 転写因子 / カルシウム |
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| Outline of Final Research Achievements |
We found Cthrc1 as a coupling factor which is transcriptionally up-regulated by bone resorption. Then, to define a mechanism in which genes are regulated by bone resorption in osteoclasts, we screened genes regulating by bone matrix and calcium by a microarray. We successfully identified Itgb3, Calcr and Atf3/7, which are highly increased in osteoclasts cultured in the presence of high calcium. Enforced expression of Atf3/7 in osteoclasts increased the expression of Cthrc1, Itgb3 and Calcr mRNA, suggesting that the expression of Atf3/7 induced by bone resorption up-regulates both bone resorbing function and bone formation by a coupling mechanism.
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