| Project/Area Number |
26670187
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA 付加体 / アダクトミクス / ゲノミクス / 発がん / 環境発がん / リスク / 暴露評価 / 分子病理疫学 / アダクトーム / DNA付加体 / 遺伝子多型 / 遺伝環境相関 / 肺がん / 胃がん / 大腸がん / 肝がん / 変異シグネチャー / 遺伝環境表現型相関 / エクソーム |
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| Outline of Final Research Achievements |
In this study, mass/charge value and liquid chromatography retention time of known 174 DNA addcuts were incoporated into LC-MS/MS machine (4000-QTRAP; Thermo). Using this adductome approach, three to 10 sites/case of gastric mucosa (31 cases) were investigated for DNA adducts measurements. Considerable numbers of known DNA adducts were present in human gastric mucosa in different quantities. Among them, tabacco-related adducts, lipid-peroxidation-induced DNA adducts, oxidative stress and alcohol related adducts, PhIP adduct was also noted. Tabacco-related adducts of current-smokers obviously increased as compared with that of non-smoker, but that of ex-smokers do not increase. It is assumed that tabacco-related adducts of ex-smoker diminish by epithelial cell turnover of the stomach. In the lung, adductome profile was different between smokers' and never smokers' lung; between lungs having adenocarcinoma and squamous cell carcinoma of smokers.
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