| Project/Area Number |
26670195
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | National Cancer Center Japan |
|---|
Principal Investigator |
Shiokawa Daisuke 国立研究開発法人国立がん研究センター, その他部局等, ユニット長 (90277278)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 大腸がん / シングルセル / 遺伝子発現解析 / Wntシグナル |
|---|
| Outline of Final Research Achievements |
In order to get an insight into the molecular basis of the tumor heterogeneity, we attempted to determine gene expression profiles of tumor cells at single cell levels. Colon adenomas as well as normal epithelium in mouse carcinogenesis models are single cell-sorted by flow cytometry, and the gene expression profiles of each cell were determined by RT-PCR assays (BioMark HD system, Fluidigm). By processing the resulting data by principal component analysis (PCA), we dissected the normal and tumor epithelium into stem and differentiated cell populations.
Notably, tumor-derived stem cells were located on distinct positions from those from normal colon on the PCA plot. Given the well-established roles of the Wnt pathway in colon cancer, we examined the expression of Wnt target genes, and found that the expression of a subset of Wnt targets was altered during carcinogenesis.
|
