| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Outline of Final Research Achievements |
Benign prostatic hyperplasia (BPH) is a very common disease over the age of 50. Since there is no suitable human model for BPH, however, we aimed to establish human model, using our super-SCID mouse system which can maintain human organs/tissues for long period. In this model, morphology and function of original BPH examined by H&E staining and immuno-histochemical staining (p63, CK8, AR, PSA, etc.) were well maintained in the biopsy tissues taken at 2, 6 and 12 month after BPH transplantation. In the mouse serum, furthermore, we can measure human PSA secreted from the xenograft by our micro-detection system.
To evaluate this human BPH model, Dutasteride was given to SCID mouse with BPH xenograft. It suppressed BPH volume and cell proliferation (Ki 63), and enhanced apoptosis (TUNEL). Thus, our new human BPH-PDX model in Super-SCID mice is very useful and valuable for medical research and drug development.
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