| Project/Area Number |
26670203
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | マゴットセラピー / 難治性創傷 / 褥瘡 / ヒロズキンバエ / バイオセラピー / 幼虫 / ハエ / 蛹化 / 抗菌物質 / 医療用ウジ治療 / CRISPR/Cas9システム |
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| Outline of Final Research Achievements |
The larvae of the green bottle fly Lucilia sericata have been employed for maggot debridement therapy (MDT). Depending on the size and depth of wound, larvae are applied 2-4 times per week and left on for a period of 24-72 h. After each period of treatment, larvae on wound should be removed because larvae pupate to be adult. Larval molting and pupation are governed by the ecdysteroids. We employed Ecdysteroid-22-oxidase (E22O), an ecdysteroid-inactivating enzyme from the entomopathogenic fungus Nomuraea rileyi, to control larval period and onset of pupation. E22O has been known to reduce ecdysteroid titer and thereby control development of several insect species. We examined inhibitory effects of E22O on pupation of L. sericata larvae. The larval stage was prolonged and pupation decreased when recombinant E22O protein was injected into second stage larvae. The data suggested that E22O-based developmental control might be potentially applicable to enhanced MDT.
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