Defense mechanism of Amoebal endosymbiont Neochlamydia on host defense against harmful Legionella infection

• Project/Area Number: 26670206
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Hokkaido University
• Principal Investigator: Yamaguchi Hiroyuki 北海道大学, 保健科学研究院, 教授 (40221650)
• Co-Investigator(Kenkyū-buntansha): MATSUO Junji 北海道大学, 大学院保健科学研究院, 講師 (50359486) ; NAKAMURA Shinji 順天堂大学, 医学研究科, 助教 (40207882) ; HAGA Sanae 北海道大学, 大学院保健科学研究院, 博士研究員 (60706505)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 共生 / 原始的なクラミジア / アメーバ / アカントアメーバ / 感染制御

Outline of Final Research Achievements

Previous our work has shown that obligate intracellular amoebal endosymbiont Neochlamydia has a critical role into host amoebal defense against harmful Legionella infection, causing amoebal killing. Here, to clarify the defense mechanism, we found a crucial piece that amoebal endosymbiotic Neochlamydia specifically could sense Legionella T4ASS (Lvh), but not T4BSS (Dot/Icm), presumably connecting to the host defense against harmful Legionella infection.

Research Products

• [Journal Article] Draft genome sequence of Chlamydia trachomatis strain 54, isolated from the urogenital tract of a male in Japan. (2015)
  • Author(s): Yamazaki T, Matsuo J, Kikuchi M, Miyamoto K, Oka K, Takahashi M, Takahashi S, Okubo T, Yamaguchi H.
  • Journal Title: Genome Announcements Volume: 3 Issue: 5
  • DOI: 10.1128/genomea.01242-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A characteristic of polymorphic membrane protein F of Chlamydia trachomatis isolated from male urogenital tracts in Japan. (2015)
  • Author(s): Yamazaki T, Matsuo J, Takahashi S, Kumagai S, Shimoda T, Abe K, Minami K, Yamaguchi H.
  • Journal Title: Journal of Infection and Chemotherapy Volume: 21 Issue: 12 Pages: 842-848
  • DOI: 10.1016/j.jiac.2015.08.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Synergistic Costimulatory Effect of Chlamydia pneumoniae with Carbon Nanoparticles on NLRP3 Inflammasome-Mediated Interleukin-1β Secretion in Macrophages (2015)
  • Author(s): Matsuo J, Nakamura S, Takeda S, Ishida K, Yamazaki T, Yoshida M, Chiba H, Hui SP, Yamaguchi H
  • Journal Title: Infect Immun Volume: 83 Issue: 7 Pages: 2917-25
  • DOI: 10.1128/iai.02968-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Draft Genome Sequence of High-Temperature-Adapted Protochlamydia sp. HS-T3, an Amoebal Endosymbiotic Bacterium Found in Acanthamoeba Isolated from a Hot Spring in Japan. (2015)
  • Author(s): Yamaguchi H, Matsuo J, Yamazaki T, Ishida K, Yagita K.
  • Journal Title: Genome Announcements Volume: 5 Issue: 1
  • DOI: 10.1128/genomea.01507-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Amoebal Endosymbiont Parachlamydia acanthamoebae Bn9 Can Grow in Immortal Human Epithelial HEp-2 Cells at Low Temperature; An In Vitro Model System to Study Chlamydial Evolution. (2015)
  • Author(s): Yamane C, Yamazaki T, Nakamura S, Matsuo J, Ishida K, Yamazaki S, Oguri S, Shouji N, Hayashi Y, Yoshida M, Yimin, Yamaguchi H.
  • Journal Title: PLOS ONE Volume: 2 Issue: 2 Pages: e0116486-e0116486
  • DOI: 10.1371/journal.pone.0116486
  • Peer Reviewed / Open Access
• [Journal Article] Chlamydia pneumoniae effector chlamydial outer protein N sequesters fructose bisphosphate aldolase A, providing a benefit to bacterial growth (2014)
  • Author(s): Ishida K, Matsuo J, Yamamoto Y, Yamaguchi H
  • Journal Title: BMC Microbiology Volume: 14 Issue: 1 Pages: 330-330
  • DOI: 10.1186/s12866-014-0330-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-γ (2014)
  • Author(s): Tomohiro Yamazaki, Junji Matsuo, Shinji Nakamura, Satoshi Oguri, Hiroyuki Yamaguchi
  • Journal Title: Journal of Infection and Chemotherapy Volume: (印刷中) Issue: 8 Pages: 460-4
  • DOI: 10.1016/j.jiac.2014.04.003
  • Peer Reviewed
• [Journal Article] High-temperature adapted primitive Protochlamydia found in Acanthamoebaisolated from a hot spring can grow in immortalized human epithelial HEp-2 cells. (2014)
  • Author(s): Aya Sampo, Junji Matsuo, Chikayo Yamane, Kenji Yagita, Shiniji Nakamura, Natsumi Shouji, Yasuhiro Hayashi, Tomohiro Yamazaki, Mitsutaka Yoshida, Miho Kobayashi, Kasumi Ishida, Hiroyuki Yamaguchi
  • Journal Title: Environmental Microbiology Volume: 16 Issue: 2 Pages: 486-497
  • DOI: 10.1111/1462-2920.12266
  • Peer Reviewed / Open Access
• [Presentation] WS13ワークショップ(Bacteria meet Viral Infection) 「Ureaplasma parvumの混合感染が生殖器粘膜面でのChlamydia trachomatisの生存性に与えるインパクト」 (2016)
  • Author(s): 山﨑智拡、松尾淳司、大久保寅彦、中村眞二、山口博之
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] 原始クラミジアNeochlamydia共生アメーバにおけるレジオネラ撃退に関わる責任分子の探索 (2016)
  • Author(s): 米田千夏、松尾淳司、山﨑智拡、大久保寅彦、中村眞二、永井広樹、山口博之
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2016-03-23
• [Presentation] トランスクリプトーム解析から探る原始的なクラミジアNeochlamydia S13が共生するアメーバのレジオネラ撃退機構 (2015)
  • Author(s): 米田千夏、山川和也、山崎智拡、松尾淳司、大久保寅彦、中村眞二、山口博之
  • Organizer: 第33 回日本クラミジア研究会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 2015-10-24
• [Presentation] Impact of Amoebal endosymbiont Neochlamydia on host defense against harmful Legionella infection and its unique defense mechanism (2015)
  • Author(s): Maita C, Matsuo J, Nakamura S, Okubo T, Nagai H, Yamaguchi H
  • Organizer: 第2回国際シンポジウム・第4回マトリョーシカ型生物学研究会(文部科学省新学術領域研究（領域提案型）)
  • Place of Presentation: 筑波大学(筑波)
  • Year and Date: 2015-09-30
• [Presentation] 原始クラミジアNeochlamydia S13が共生するアメーバはレジオネラを撃退する：トランスクリプトーム解析による責任遺伝子の探索 (2015)
  • Author(s): 米田千夏、松尾淳司、山崎智拡、大久保寅彦、中村眞二、永井宏樹、山口博之
  • Organizer: 第82回日本細菌学会北海道支部学術総会
  • Place of Presentation: 北海道医療大学(札幌)
  • Year and Date: 2015-09-05
• [Presentation] 特別講演 「クラミジアの基礎: クラミジアの持続感染とは何か」 (2015)
  • Author(s): 山口博之
  • Organizer: 第24回北海道性感染症研究会
  • Place of Presentation: 札幌第一三共株式会社(札幌)
  • Year and Date: 2015-07-04
  • Invited
• [Presentation] Computational based prediction of novel type III secreted effectors in pathogenic chlamydiae (2015)
  • Author(s): Yamazaki T, Ohba H, Takagi N, Matsuo J, Yamaguchi H
  • Organizer: 115th General Meeting American Society for Microbiology
  • Place of Presentation: New Orleans Ernest N. Morial Convention Center (New Orleans, USA)
  • Year and Date: 2015-05-30
  • Int'l Joint Research
• [Presentation] Polymorphisms in polymorphic membrane protein F (PmpF) of Chlamydia trachomatis isolated from male (2015)
  • Author(s): Matsuo J, Kumagai S, Yamazaki T, Takahashi S, Yamaguchi H
  • Organizer: 115th General Meeting American Society for Microbiology
  • Place of Presentation: New Orleans Ernest N. Morial Convention Center (New Orleans, USA)
  • Year and Date: 2015-05-30
  • Int'l Joint Research
• [Presentation] Intracellular growth mechanism of amoebal endosymbiont environmental chlamydiae Parachlamydia Bn9 in immortalized human epithelial HEp-2 cells at low temperature 30°C (2015)
  • Author(s): Yamaguchi H, Yamane C, Yamazaki T, Ishida K, Matsuo J, Nakamura S
  • Organizer: 115th General Meeting American Society for Microbiology
  • Place of Presentation: New Orleans Ernest N. Morial Convention Center (New Orleans, USA)
  • Year and Date: 2015-05-30
  • Int'l Joint Research
• [Presentation] Chlamydia pneumoniae effector CopN sequesters a glycolytic enzyme, frucutose-bisphosphate aldolase A, providing a benefit to the bacterial growth (2015)
  • Author(s): 石田香澄、松尾淳司、中村眞二、山口博之
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Computational prediction of novel type III effectors in pathogenic Chlamydiae and construction of GFP-fusion expression vector targeting the effectors (2015)
  • Author(s): 山﨑智拡、松尾淳司、山口博之
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Synergistic effect of chlamydiale with carbon nanoparticules on IL-1b secreation via NLRP3 inflammasome (2015)
  • Author(s): 松尾淳司、中村眞二、山口博之
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Lessons from primitive chlamydiae as endosymbionts in eukaryotes (2015)
  • Author(s): 山口博之
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Chlamydia pneumoniae stimulation with carbon nanoparticles synergistically enhances IL-1β secretion from macrophages (2014)
  • Author(s): J. Matsuo, S. Takeda, S. Nakamura, H. Chiba, H. Yamaguchi
  • Organizer: ASM2014 (アメリカ微生物学会)
  • Place of Presentation: ボストン
  • Year and Date: 2014-05-17 – 2015-05-20
• [Presentation] Chlamydial effector CopN interacts with human fructose-bisphosphate aldolase A (2014)
  • Author(s): Kasumi Ishida, Junji Matsuo, Sumire Yamazaki, Shinji Nakamura, Hiroyuki Yamaguchi
  • Organizer: ASM2014 (アメリカ微生物学会)
  • Place of Presentation: ボストン
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] Intracellular growth mechanism of amoebal endosymbiont environmental chlamydiae Parachlamydia Bn9 in immortalized human epithelial HEp-2 cells at low temperature 30°C (2014)
  • Author(s): Hiroyuki Yamaguchi, Chikayo Yamane, Tomohiro Yamazaki , Kasumi Ishida, Junji Matsuo, Shinji Nakamura
  • Organizer: ASM2014 (アメリカ微生物学会)
  • Place of Presentation: ボストン
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] High-temperature adapted amoebal endosymbiont Protochlamydia found in HS-T3 Acanthamoeba isolated from a hot spring can grow in immortalized human epithelial HEp-2 cells (2014)
  • Author(s): Tomohiro Yamazaki, Aya Sampo, Junji Matsuo, Chikayo Yamane1, Shinji Nakamura, Kasumi Ishida, Kenji Yagita, Hiroyuki Yamaguchi
  • Organizer: ASM2014 (アメリカ微生物学会)
  • Place of Presentation: ボストン
  • Year and Date: 2014-05-17 – 2014-05-20