| Project/Area Number |
26670211
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WACHINO Jun-ichi 名古屋大学, 大学院医学系研究科, 特任講師 (00535651)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 薬剤耐性遺伝子 / 水平伝播 / Acinetobacter baumannii / メンブレンベシクル / 薬剤耐性遺伝子伝達 |
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| Outline of Final Research Achievements |
Bacterial outer membrane vesicles (OMVs) contain several biological materials such as DNA, RNA, and proteins. We hypothesized that antibiotic resistance genes, included in OMVs, have a role in the transfer of these genes to other bacterial cells. To test this, we purified OMVs from the culture supernatant of a multidrug resistant Acinetobacter baumannii strain (donor cells), and applied them to an antibiotic susceptible A. baumannii strain (recipient cells). We obtained several transformants that acquired antibiotic resistance genes derived from the donor A. baumannii cells. Whole genome sequence analyses of the transformants revealed that DNA fragments longer than 10-kb, and those including antibiotic resistance genes, could be inserted into the chromosomal DNA of recipient cells. These DNA fragments appeared to be inserted via homologous recombination.
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