Study of gliding movement and epithelial-cell adhesion mechanism found in unclassified pnumonic bacteria of CFB phylum

• Project/Area Number: 26670217
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ike Fumio 国立研究開発法人理化学研究所, バイオリソースセンター, 専任研究員 (40183157)
• Co-Investigator(Kenkyū-buntansha): 小久保 年章 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 技術安全部, 課長(定常) (10425663)
• Research Collaborator: KAJITA AYAKO 国立研究開発法人理化学研究所, バイオリソースセンター, 人材派遣 ; SAKAMOTO MITSUO 国立研究開発法人理化学研究所, バイオリソースセンター, 専任研究員 (50321766)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: カー(CAR)バチルス / フィロバクテリウム・ローデンティウム / グラム陰性菌 / マウス / 呼吸器感染症 / 肺炎病原菌 / 細胞付着機構 / 滑走運動 / CARバチルス / Filobacterium rodentium / 呼吸器感染症原因菌 / ラット / ９型分泌装置 / ゲノム編集 / 培養法改良

Outline of Final Research Achievements

The cilia-associated respiratory bacillus (CARB), formerly unclassified, extracellular, gram-negative filamentous bacterium, colonizes the ciliated respiratory epithelium of rodents and causes persistent respiratory diseases. Previously we improved culture method of CARB of SMR-C strain, extracted genomic DNA from SMR-C and decided its full genome sequences with help of Prof. Hattori and Dr. Oshima at Tokyo University. Genomic analysis showed 40% of annotated genes were not found on the DDBJ/EMBL/Genbank database and we could not speculate virulent factor genes of CARB from its genome.
In this study, we tried to clarify anchoring system of CARB onto epithelial cells, especially focusing on gliding-related genes. Meanwhile we gave scientific name, Filobacterium rodentium, to SMR-C strain also with new genus and family names. We also found F. rodentium displayed stress-induced phase variations including biofilm formation. These findings will help to promote the study of F. rodentium.

Research Products

• [Journal Article] 齧歯類分離CAR バチルスの新学名, Filobacterium rodentiumについて (2016)
  • Author(s): 池 郁生
  • Journal Title: 実験動物ニュース Volume: 65 Pages: 65-68
  • Open Access
• [Journal Article] Analysis of genes involved in the PI3K/Akt pathway in radiation- and MNU-induced rat mammary carcinomas (2016)
  • Author(s): Kaye Showler , Mayumi Nishimura , Kazuhiro Daino , Tatsuhiko Imaoka , Yukiko Nishimura , Takamitsu Morioka , Benjamin J. Blyth , Toshiaki Kokubo , Masaru Takabatake , Maki Fukuda , Hitomi Moriyama , Shizuko Kakinuma , Masahiro Fukushi , Yoshiya Shimada
  • Journal Title: Journal of radiation research Volume: 58 Issue: 2 Pages: 183-194
  • DOI: 10.1093/jrr/rrw097
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] "Filobacterium rodentium gen. nov., sp. nov., a member of Filobacteriaceae fam. nov. within the phylum Bacteroidetes, and includes microaerobic filamentous bacterium isolated from rodent respiratory disease specimens" (2016)
  • Author(s): Ike F*, Sakamoto M, Ohkuma M, Kajita A, Matsushita S, Kokubo T.
  • Journal Title: Int. J. Syst. Evol. Microbiol. Volume: 66 Issue: 1 Pages: 150-157
  • DOI: 10.1099/ijsem.0.000685
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] "Evaluation of X-ray doses and their corresponding biological effects on experimental animals in cone-beam micro-CT scans (R-mCT2)" (2016)
  • Author(s): Miyahara N, Kokubo T, Hara Y, Yamada A, Koike T, Arai Y.
  • Journal Title: Radiol. Phys. Technol. Volume: 9 Issue: 1 Pages: 60-68
  • DOI: 10.1007/s12194-015-0334-1
  • Peer Reviewed
• [Journal Article] Biological Measures to Minimize the Risk of Radiotherapy-Associated Second Malignant Neoplasm: a Research Perspective (2016)
  • Author(s): 今岡 達彦．石井 伸昌，川口 勇生，武田 志乃，土居 主尚，臺野 和広，中西 郁夫，田上 恵子，小久保 年章，森岡 孝満，細木 彩夏，高畠 賢，吉永 信治
  • Journal Title: International Journal of Radiation Biology Volume: 92 Issue: 6 Pages: 1-13
  • DOI: 10.3109/09553002.2016.1152413
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Polymorphisms Influencing Expression of Dermonecrotic Toxin in Bordetella bronchiseptica. (2015)
  • Author(s): Okada K, Abe H, Ike F, Ogura Y, Hayashi T, Fukui-Miyazaki A, Nakamura K, Shinzawa N, Horiguchi Y.
  • Journal Title: PLoS One Volume: 10 Issue: 2 Pages: e0116604-e0116604
  • DOI: 10.1371/journal.pone.0116604
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quantifying initial cellular events of mouse radiation lymphomagenesis and its tumor prevention in vivo by positron emission tomography and magnetic resonance imaging (2015)
  • Author(s): Hasegawa S, Morokoshi Y, Tsuji A B, Kokubo T, Aoki I, Furukawa T, Zhang M-R, and Saga T
  • Journal Title: Mol. Oncol. Volume: 9 Issue: 3 Pages: 740-748
  • DOI: 10.1016/j.molonc.2014.11.009
  • Peer Reviewed / Open Access
• [Journal Article] Ionizing radiation, inflammation, and their interactions in colon carcinogenesis in Mlh1-deficient mice (2015)
  • Author(s): Morioka T, Miyoshi-Imamura T, Blyth BJ, Kaminishi M, Kokubo T, Nishimura M, Kito S, Tokairin Y, Tani S, Murakami-Murofushi K, Yoshimi N, Shimada Y, Kakinuma S
  • Journal Title: Cancer Sci Volume: 106(3) Issue: 3 Pages: 217-226
  • DOI: 10.1111/cas.12591
  • Peer Reviewed / Open Access
• [Journal Article] Detection of murine norovirus by reverse transcription loop-mediated isothermal amplification (2014)
  • Author(s): Hanaki, K., Ike, F., Kajita, A., Yasuno, W., Yanagiba, M., Goto, M., Sakai, K., Ami, Y., Kyuwa, S
  • Journal Title: J Virol Methods Volume: 204C Pages: 17-24
  • DOI: 10.1016/j.jviromet.2014.03.025
  • Peer Reviewed / Open Access
• [Journal Article] A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus (2014)
  • Author(s): Hanaki, K., Ike, F., Kajita, A., Yasuno, W., Yanagiba, M., Goto, M., Sakai, K., Ami, Y., Kyuwa, S
  • Journal Title: PLoS One Volume: 9 Issue: 5 Pages: e98108-e98108
  • DOI: 10.1371/journal.pone.0098108
  • Peer Reviewed / Open Access
• [Journal Article] CARバチルス感染症 (2014)
  • Author(s): 池 郁生
  • Journal Title: 実験動物ニュース Volume: 63 (3) Pages: 50-55
  • Open Access
• [Presentation] Trial of gellan gum plate to culture Filobacterium rodentium (2017)
  • Author(s): 池 郁生
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター, 宮城県仙台市
  • Year and Date: 2017-03-19
• [Presentation] 培養法検討から始まった"CARバチルス"学名命名 (2016)
  • Author(s): 池 郁生
  • Organizer: 第93回実験動物コンファレンス
  • Place of Presentation: 日本獣医生命科学大学Ｃ棟, 東京都三鷹市
  • Year and Date: 2016-12-17
  • Invited
• [Presentation] The importance of basic research in infectious diseases of laboratory animals (2016)
  • Author(s): Ike F
  • Organizer: 7th AFLAS Congress
  • Place of Presentation: Raffles City Convention Center, Singapore
  • Year and Date: 2016-11-07
  • Int'l Joint Research / Invited
• [Presentation] Filobacterium rodentium, a New Name for CAR Bacillus Rodent Isolates (2016)
  • Author(s): Ike F, Kajita A, Yosiki A, Sakamoto M, Kokubo T.
  • Organizer: 67th AALAS National Meeting
  • Place of Presentation: Charlotte Convention Center, Charlotte, USA
  • Year and Date: 2016-10-30
  • Int'l Joint Research
• [Presentation] バクテロイデス門に新たに新科・新属・新種として提案したFilobacterium rodentiumについて (2016)
  • Author(s): 池 郁生, 梶田亜矢子, 吉木 淳, 坂本光央, 小久保年章
  • Organizer: 第159回日本獣医学会総会学術集会
  • Place of Presentation: 日本大学生物資源科学部, 神奈川県藤沢市
  • Year and Date: 2016-09-06
• [Presentation] Physical stress induces phase variations in Filobacterium rodentium, rodent pneumonic bacterium (2016)
  • Author(s): Ike F, Kajita A, Kokubo T.
  • Organizer: Biofilms 7
  • Place of Presentation: University of Porto, Porto, Portugal
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Filobacterium rodentium, a new name of the cilia-associated respiratory bacillus (2016)
  • Author(s): Ike F, Kajita A, Yoshiki A, Sakamoto M, Kokubo T.
  • Organizer: 13th FELASA Congress
  • Place of Presentation: Square Brussels Meeting Centre, Brussels, Belgium
  • Year and Date: 2016-06-13
  • Int'l Joint Research
• [Presentation] "CAR バチルス"の新学名Filobacterium rodentiumについて (2016)
  • Author(s): 池 郁生, 坂本光央, 大熊盛也, 梶田亜矢子, 松下 悟, 吉木 淳, 小久保年章
  • Organizer: 第63回実験動物学会総会
  • Place of Presentation: ミューザ川崎シンフォニーホール, 神奈川県川崎市
  • Year and Date: 2016-05-18
• [Presentation] 現場からみた微生物検査におけるイノベーション (2016)
  • Author(s): 池 郁生
  • Organizer: 第63回実験動物学会総会
  • Place of Presentation: ミューザ川崎シンフォニーホール, 神奈川県川崎市
  • Year and Date: 2016-05-18
  • Invited
• [Presentation] "マウスのCARバチルス感染症" (2016)
  • Author(s): 池 郁生
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター, 大阪府大阪市
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] "CARバチルスに学名がつきました！" (2015)
  • Author(s): 池 郁生, 坂本光央, 小久保年章.
  • Organizer: 第128回関西実験動物研究会
  • Place of Presentation: 京都大学, 京都府京都市
  • Year and Date: 2015-12-04
• [Presentation] "免疫不全動物の飼育管理" (2015)
  • Author(s): 池 郁生
  • Organizer: 第33回九州実験動物研究会総会/第35回日本実験動物技術者協会九州支部研究発表会
  • Place of Presentation: 九州大学, 福岡県福岡市
  • Year and Date: 2015-11-07
  • Invited
• [Presentation] "CARバチルスによる結核菌コード様構造物の形成" (2015)
  • Author(s): 池 郁生, 梶田亜矢子, 小久保年章.
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部, 青森県十和田市
  • Year and Date: 2015-09-07
• [Presentation] "理研BRCにおける提供用免疫不全系統マウスの飼育、ならびに実験エリアでの同種マウスの維持管理" (2015)
  • Author(s): 池 郁生
  • Organizer: 第158回日本獣医学会総会学術集会
  • Place of Presentation: 北里大学獣医学部, 青森県十和田市
  • Year and Date: 2015-09-07
  • Invited
• [Presentation] "CARバチルスの系統分類" (2015)
  • Author(s): 池 郁生, 梶田亜矢子, 坂本光央, 小久保年章.
  • Organizer: 第62回日本実験動物学会総会
  • Place of Presentation: 京都テルサ, 京都府京都市
  • Year and Date: 2015-05-28
• [Presentation] "gBlocks-based CrisprCas9システムによるノックアウトマウス作出条件の検討" (2015)
  • Author(s): 塚本智史, 伊林恵美, 和田彩子, 道川祐市, 安井孝彰, 矢野実, 鬼頭靖司, 小久保年章.
  • Organizer: 第62回日本実験動物学会総会
  • Place of Presentation: 京都テルサ, 京都府京都市
  • Year and Date: 2015-05-28
• [Presentation] Description of Gram-negative rodent pneumonic bacteria, 'CAR bacillus' (2015)
  • Author(s): Ike F, Sakamoto M.
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場, 岐阜市
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] What we have drawn from full-genome sequencing of rodent pneumonic CAR bacillus (2014)
  • Author(s): Ike F, Oshima K, Hattori M, Kokubo T.
  • Organizer: 13th Awaji International Forum on Infection and Immunity in Nara
  • Place of Presentation: Nara Prefectural New Public Hall, Nara, Japan
  • Year and Date: 2014-09-23 – 2014-09-26
• [Presentation] CARバチルス培養における鉄の影響 (2014)
  • Author(s): 池 郁生, 梶田亜矢子, 小久保年章
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 国立大学法人北海道大学, 札幌市
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 齧歯類肺炎菌 CAR バチルスのゲノム解析から分かってきたこと (2014)
  • Author(s): 池 郁生, 大島健志朗, 服部正平, 小久保年章
  • Organizer: 第8回細菌学若手コロッセウム
  • Place of Presentation: ホテルニセコいこいの村, 北海道虻田郡ニセコ町
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] Stress-induced aggregate formation by Gram-negative rodent pneumonic bacteria, CAR bacillus (2014)
  • Author(s): Ike F, Kajita A, Sakata H, Sakamoto M, Kokubo T.
  • Organizer: IUMS 2014, XIV International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] CARバチルスによるバイオフィルム形成 (2014)
  • Author(s): 池 郁生, 梶田亜矢子, 坂田ひろみ, 小久保年章
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌コンベンションセンター, 札幌市
  • Year and Date: 2014-05-15 – 2014-05-17
• [Book] 「系統共有のためのリソース」マウス表現型解析スタンダード 系統の選択、飼育環境、臓器・疾患別解析のフローチャートと実験例 (2016)
  • Author(s): 吉木 淳, 綾部信哉, 池 郁生, 仲柴俊昭, 中田初美, 平岩典子
  • Total Pages: 347
  • Publisher: 株式会社 羊土社
• [Remarks] ‘CAR bacillus’ finally got the binomial name
  • URL: http://mus.brc.riken.jp/en/outofdate/news-151207
• [Remarks] マウス・ラットの肺炎病原体として新「科」微生物を命名 －発見以来35年ぶりに学名つく－
  • URL: http://www.riken.jp/pr/press/2015/20151207_1/
• [Remarks] 理研BRC実験動物開発室
  • URL: http://mus.brc.riken.jp/ja/