| Project/Area Number |
26670218
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Yamamoto Shouji 国立感染症研究所, 細菌第一部, 主任研究官 (80469957)
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| Co-Investigator(Renkei-kenkyūsha) |
MITOBE JIRO 国立感染症研究所, 細菌第一部, 主任研究官 (40333364)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 細菌間コミュニケーション / 低分子RNA / RNA取り込み能 / RIVET / 調節性ノンコーディングRNA |
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| Outline of Final Research Achievements |
This study was done to develop a DNA memory that records cell-to-cell transfer of bacterial small RNA (sRNA). This system consists of two distinct elements: one is a sensor of specific sRNA and the other is a memory of sensed information. The sensor is a translational fusion of the site-specific recombinase gene tnpR with a target gene of the sRNA while the memory is a DNA cassette containing a chloramphenicol (Cm) resistance gene and a levansucrase gene, which is flanked by TnpR-binding sites. In response to the presence of a specific sRNA, the expression of TnpR is activated. Synthesized TnpR in turn promotes excision of the DNA cassette, which causes sensitivity to Cm and confers resistance to sucrose. I constructed a series of sensors/memories for TfoR, the competence-activating sRNA in Vibrio cholerae, and evaluated performances. However, their low specificities and sensitivities were problematic. It is necessary to improve these issues for developing this type of memory device.
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