| Project/Area Number |
26670219
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Miyazaki Kentaro 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60344123)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | リボソーム / 抗生物質 / 耐性 / 遺伝子変異 / 大腸菌 / スペクチノマイシン |
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| Outline of Final Research Achievements |
We have constructed a metagenomic library of 16S rRNA, which complemented the growth of the null mutant of Escherichia coli. When we used a forward primer, which could generate unwanted mismatch between 19 and 916 bases, we found some of the mutant strains happened to obtain a spectinomycin resistance. When a primer, which would not generate mismatch at the site, no such resistance was observed. From the metagenomic library of the 16S rRNA genes, we identified a various antibiotic resistant strains: spectinomycin (4 clones); G418 (10 clones); tetracycline (2 clones); and apramycin (1 clone). These clones contained some new nucleotides that provide the resistance on the host strain.
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