| Project/Area Number |
26670221
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Saito Izumu 東京大学, 医科学研究所, 教授 (70158913)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | B型肝炎ウイルス / split Cre / α相補 / 蛍光スクリーニング / HBV創薬 / Ｂ型肝炎ウイルス / preS / ウイルスゲノム複製 |
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| Outline of Final Research Achievements |
We constructed genomes of hepatitis B virus (HBV), in which PreS region were replaced by split Cre’s (α-Cre andβ-Cre for first and latter halves, respectively), while overlapping Pol frame is maintained. Simultaneous expression of both the split Cre’s resulted in the Cre activity. The replacement does not exceed the genome size that can be replicated. The HBV genome described here expressed split Cre and, when the other split Cre was supplied from outside the genome, functional Cre activity was observed and turn on the GFP expression present outside. Because the replication of HBV genomes constructed here have not sufficiently been examined within the term of this study, further experiments are needed to confirm replication of these genomes quantitatively. Moreover, more information could be available if adenovirus vector systems are used instead of transfection. When they are confirmed, these HBV genomes must be useful for fluorescent screening of ant-HBV medicine.
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