| Project/Area Number |
26670224
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO MASAHIKO 浜松医科大学, 医学部, 助教 (50385423)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | C型肝炎ウイルス / ウイルス複製 / ATP / ミトコンドリア / 複製 |
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| Outline of Final Research Achievements |
Replication of the virus genome is a physiological mechanism that is known to require energy for operations. However, mechanisms for how ATP, the major energy currency of living cells, can be recruited to the site for the viral replication are unknown.
In this study, since mitochondria play a central role in ATP metabolism and is known to localize near the membranous web in certain conditions, we analyzed the subcellular localization of the FRET and fluorescence signals detected in cells expressing HCV-ATeam subgenome with that of mitochondria. Possible viral replication complexes, foci reflecting high ATP levels, did not co-localize with, but were localized adjacent to mitochondria in the viral replicating cells. This may suggest that ATP can be directly supplied from mitochondria to the sites of viral RNA replication in cells.
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