| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To determine the molecular basis for the regulation of gene mutation rate in influenza A viruses, we generated recombinant influenza A virus library possessing random mutation(s) in the PB1 gene. We selected virus clones exhibiting (1) reduced sensitivity to antiviral and (2) enhanced stability of the reporter gene expression. Through validation of the mutation rate of the selected virus clones by means of reporter assays and sequencing of the PB1 genes, we identified the PB1 gene mutations that enhance and reduce the viral mutation rates, respectively.
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