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Search for RNA Biomarker in Aqueous Humor from patients by Conprehensive Transcriptome Analysis

Research Project

Project/Area Number 26670263
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied pharmacology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Tanaka Yuji  国立研究開発法人理化学研究所, 情報基盤センター, センター研究員 (40625513)

Co-Investigator(Kenkyū-buntansha) 河合 純  国立研究開発法人理化学研究所, 予防医療・診断技術開発プログラム, 副プログラムディレクター (30391923)
國方 彦志  東北大学, 医学系研究科, 准教授 (40361092)
藤田 幸輔  東北大学, 医学系研究科, 助手 (80708115)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordscirculating miRNA / 前房水 / 網羅的解析 / 次世代シーケンサー / circulating RNA / 短鎖RNA-Seq / 短鎖RNA / バイオマーカー
Outline of Final Research Achievements

We extracted a trace amount of RNA from anterior aqueous humor from patients with ophthalmopathy of just a few 100 μL and tried comprehensive RNA analysis using next generation sequencer etc. About 360 kinds of microRNAs were successfully detected from one clinical specimen, and about one third of them were commonly detected in eight cataract specimens. Of these, 75% of the top 40 in expression levels were consistent with other studies reported during the study period. As a study that tried comprehensive analysis individually without mixing samples, our microarray analysis reported at the beginning of the research period is the first time, and the analysis scale by the next generation sequencer is the largest. In addition, as a surrounding study, we have examined microRNA analysis method and pathology model evaluation method and obtained basic knowledge.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2016 2014

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results)

  • [Journal Article] Real-time imaging of RGC death with a cell-impermeable nucleic acid dyeing compound after optic nerve crush in a murine model.2016

    • Author(s)
      Tsuda S, Tanaka Y, Kunikata H, Yokoyama Y, Yasuda M, Ito A, Nakazawa T
    • Journal Title

      Exp Eye Res

      Volume: 146 Pages: 179-188

    • DOI

      10.1016/j.exer.2016.03.017

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system.2014

    • Author(s)
      Tanaka Y, Tsuda S, Kunikata H, Sato J, Kokubun T, Yasuda M, Nishiguchi KM, Inada T, Nakazawa T.
    • Journal Title

      Sci Rep.

      Volume: 4 Issue: 1 Pages: 5089-5089

    • DOI

      10.1038/srep05089

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neuroprotective effect against axonal damage-induced retinal ganglion cell death in apolipoprotein E-deficient mice through the suppression of kainate receptor signaling.2014

    • Author(s)
      10.Omodaka K, Nishiguchi KM, Yasuda M, Tanaka Y, Sato K, Nakamura O, Maruyama K, Nakazawa T.
    • Journal Title

      Brain Res

      Volume: 1586 Pages: 203-212

    • DOI

      10.1016/j.brainres.2014.08.053

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Retinal transcriptome profiling at transcription start sites: a cap analysis of gene expression early after axonal injury2014

    • Author(s)
      Yasuda M, Tanaka Y, Nishiguchi KM, Ryu M, Tsuda S, Maruyama K, Nakazawa T
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 982-982

    • DOI

      10.1186/1471-2164-15-982

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Artemin augments survival and axon regeneration in axotomized retinal ganglion cells.2014

    • Author(s)
      Omodaka K, Kurimoto T, Nakamura O, Sato K, Yasuda M, Tanaka Y, Himori N, Yokoyama Y, Nakazawa T.
    • Journal Title

      J Neurosci Res.

      Volume: 92 Issue: 12 Pages: 1637-1646

    • DOI

      10.1002/jnr.23449

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access

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Published: 2014-04-04   Modified: 2019-12-27  

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