Search for RNA Biomarker in Aqueous Humor from patients by Conprehensive Transcriptome Analysis

• Project/Area Number: 26670263
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanaka Yuji 国立研究開発法人理化学研究所, 情報基盤センター, センター研究員 (40625513)
• Co-Investigator(Kenkyū-buntansha): 河合 純 国立研究開発法人理化学研究所, 予防医療・診断技術開発プログラム, 副プログラムディレクター (30391923) ; 國方 彦志 東北大学, 医学系研究科, 准教授 (40361092) ; 藤田 幸輔 東北大学, 医学系研究科, 助手 (80708115)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: circulating miRNA / 前房水 / 網羅的解析 / 次世代シーケンサー / circulating RNA / 短鎖RNA-Seq / 短鎖RNA / バイオマーカー

Outline of Final Research Achievements

We extracted a trace amount of RNA from anterior aqueous humor from patients with ophthalmopathy of just a few 100 μL and tried comprehensive RNA analysis using next generation sequencer etc. About 360 kinds of microRNAs were successfully detected from one clinical specimen, and about one third of them were commonly detected in eight cataract specimens. Of these, 75% of the top 40 in expression levels were consistent with other studies reported during the study period. As a study that tried comprehensive analysis individually without mixing samples, our microarray analysis reported at the beginning of the research period is the first time, and the analysis scale by the next generation sequencer is the largest. In addition, as a surrounding study, we have examined microRNA analysis method and pathology model evaluation method and obtained basic knowledge.

Research Products

• [Journal Article] Real-time imaging of RGC death with a cell-impermeable nucleic acid dyeing compound after optic nerve crush in a murine model. (2016)
  • Author(s): Tsuda S, Tanaka Y, Kunikata H, Yokoyama Y, Yasuda M, Ito A, Nakazawa T
  • Journal Title: Exp Eye Res Volume: 146 Pages: 179-188
  • DOI: 10.1016/j.exer.2016.03.017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system. (2014)
  • Author(s): Tanaka Y, Tsuda S, Kunikata H, Sato J, Kokubun T, Yasuda M, Nishiguchi KM, Inada T, Nakazawa T.
  • Journal Title: Sci Rep. Volume: 4 Issue: 1 Pages: 5089-5089
  • DOI: 10.1038/srep05089
  • Peer Reviewed / Open Access
• [Journal Article] Neuroprotective effect against axonal damage-induced retinal ganglion cell death in apolipoprotein E-deficient mice through the suppression of kainate receptor signaling. (2014)
  • Author(s): 10.Omodaka K, Nishiguchi KM, Yasuda M, Tanaka Y, Sato K, Nakamura O, Maruyama K, Nakazawa T.
  • Journal Title: Brain Res Volume: 1586 Pages: 203-212
  • DOI: 10.1016/j.brainres.2014.08.053
  • Peer Reviewed
• [Journal Article] Retinal transcriptome profiling at transcription start sites: a cap analysis of gene expression early after axonal injury (2014)
  • Author(s): Yasuda M, Tanaka Y, Nishiguchi KM, Ryu M, Tsuda S, Maruyama K, Nakazawa T
  • Journal Title: BMC Genomics Volume: 15 Issue: 1 Pages: 982-982
  • DOI: 10.1186/1471-2164-15-982
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Artemin augments survival and axon regeneration in axotomized retinal ganglion cells. (2014)
  • Author(s): Omodaka K, Kurimoto T, Nakamura O, Sato K, Yasuda M, Tanaka Y, Himori N, Yokoyama Y, Nakazawa T.
  • Journal Title: J Neurosci Res. Volume: 92 Issue: 12 Pages: 1637-1646
  • DOI: 10.1002/jnr.23449
  • Peer Reviewed / Open Access