Efficient induction of in vivo RNA interference by designing exosome-tropic siRNA derivatives
| Project/Area Number |
26670266
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Kyoto University |
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Principal Investigator |
Takahashi Yuki 京都大学, 薬学研究科(研究院), 助教 (00547870)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinobu 京都大学, 大学院薬学研究科, 教授 (30171432)
NISHIKAWA Makiya 京都大学, 大学院薬学研究科, 准教授 (40273437)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | エキソソーム / ラジオアイソトープ / DDS / SELEX法 |
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| Outline of Final Research Achievements |
In the current study, for the goal of the development of drug delivery system by using exosomes, a method to quantitatively evaluate in vivo tissue distribution of exosomes was developed, and three ultracentrifugation-based exosome collection methods were compared to one another. We selected a biotin binding protein (streptavidin: SAV) and an exosome-tropic protein (lactadherin: LA) to obtain a fusion protein SAV-LA. Radio-labeled exosomes were prepared by using SAV-LA and radioactive biotin derivatives, and tissue distribution of the exosomes was quantitatively evaluated by using the labeled exosomes. By comparing the three ultracentrifugation-based exosome collection methods, we concluded that a density gradient ultracentrifugation method was the best method to collect exosomes with minimal aggregates and in a good quality.
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Report
(3 results)
Research Products
(10 results)
