| Project/Area Number |
26670274
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Gunma University |
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Principal Investigator |
yagi hisako 群馬大学, 医学部附属病院, 助教 (50717832)
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| Research Collaborator |
TAKIZAWA Takumi
AIZAWA Akira
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | アレルギー検査 / 好塩基球 / 特異的IgE抗体 / Ⅰ型アレルギー / アレルギー / 抗原特異的IgE抗体検査 / RAST / 好塩基球活性化試験 / IgE / ＲＡＳＴ / ＩｇＥ |
|---|
| Outline of Final Research Achievements |
The purpose of this study was to develop a novel allergen test using a basophil cell line that possesses both the simplicity of the serum specific IgE measurement and the specificity of basophil activation test (BAT) for the diagnosis of IgE-mediated allergic diseases. We established an efficient differentiating culture system of an immature basophil cell line and subcloned a cell line that highly expresses Fcε receptor I. We incubated the cells with serum from patients with Japanese cedar pollinosis, activated it with the allergen, and determined the expression of basophil activation markers, such as CD164, with flow cytometry. Since spontaneous activation of the cells impaired the detection of allergen-specific activation, we measured the activity of tryptase, which is released upon degradation. The tryptase activity increased upon allergen stimulation, indicating that this new method was simple and easy, while complementing the drawbacks of a BAT assay.
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