Preemptive medicine for allergic disorders: Significance of bacterial flora on the nasal cavity in atopic dermatitis

• Project/Area Number: 26670275
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: MAEKAWA MASATO 浜松医科大学, 医学部, 教授 (20190291)
• Co-Investigator(Kenkyū-buntansha): 戸倉 新樹 浜松医科大学, 医学部, 教授 (00172156)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: アトピー性皮膚炎 / ブドウ球菌 / 皮膚常在菌 / 鼻腔常在菌 / 16S rRNA解析 / 菌株解析 / POT法 / 質量分析計 / 黄色ブドウ球菌 / 鼻腔常在菌叢 / マイクロバイオーム / 先制医療

Outline of Final Research Achievements

Two specimens were picked up from diseased skin and nasal cavity of 25 patients with atopic dermatitis. 16S rRNA analysis showed the preponderance of Actinobacteria and Firmicutes on nasal cavity and Firmicutes and Proteobacteria on skin at the Phylum level. Three colonies of Staphylococcus were cultured and analyzed by POT (PCR based-open reading frame typing. The same POT type strains tended to be confirmed in both skin and nasal cavity of each patient.
The colonized bacteria are recruited from the nasal cavity of the patients, not environment, and are related to the deterioration of dermatitis.

Research Products

• [Journal Article] DNA methylation analysis in malignant pheochromocytoma and paraganglioma. (2017)
  • Author(s): Oishi T, Iino K, Okawa Y, Kakizawa K, Matsunari S, Yamashita M,Taniguchi T, Maekawa M, Suda T,Oki Y
  • Journal Title: Journal of Clinical & Translational Endocrinology Volume: 7 Pages: 12-20
  • DOI: 10.1016/j.jcte.2016.12.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of serum bone alkaline phosphatase activity in patients with liver disease: Comparison between electrophoresis and chemiluminescent enzyme immunoassay. (2016)
  • Author(s): Zhan F, Watanabe Y, Shimoda A, Hamada E, Kobayashi Y, Maekawa M
  • Journal Title: Clin Chim Acta. Volume: 460 Pages: 40-45
  • DOI: 10.1016/j.cca.2016.06.008
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Nonspecific interstitial pneumonia preceding diagnosis of collagen vascular disease. (2016)
  • Author(s): Kono M, Nakamura Y, Yoshimura K, Enomoto Y, Oyama Y, Hozumi H, Enomoto N,Fujisawa T, Inui N, Hamada E, Colby TV, Maekawa M, Suda T
  • Journal Title: Respir Med. Volume: 117 Pages: 40-47
  • DOI: 10.1016/j.rmed.2016.05.030
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pre-examination factors affecting molecular diagnostic test results and interpretation: A case-based approach. (2016)
  • Author(s): Payne DA, Baluchova K, Peoc'h KH, van Schaik RH, Chan KC, Maekawa M, Mamotte C, Russomando G, Rousseau F, Ahmad-Nejad P; IFCC Committee for Molecular Diagnostics (C-MD)
  • Journal Title: Clin Chim Acta. Volume: pii: S0009-8981(16) Pages: 30263-30267
  • DOI: 10.1016/j.cca.2016.06.018
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Increased levels of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein in idiopathic pulmonary fibrosis. (2016)
  • Author(s): Kono M, Nakamura Y, Oyama Y, Mori K, Hozumi H, Karayama M, Hashimoto D, Enomoto N, Fujisawa T, Inui N, Yamada M, Hamada E, Colby TV, Maekawa M, Suda T
  • Journal Title: Respir Med. Volume: 115 Pages: 46-52
  • DOI: 10.1016/j.rmed.2016.04.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fetal Environment and Glycosylation Status in Neonatal Cord Blood: A Comprehensive Mass Spectrometry-based Glycosylation Analysis. (2016)
  • Author(s): Sato R, Tsuchiya KJ, Matsuzaki H, Takei N, Itoh H, Kanayama N, Suda T, Watanabe H, Ohashi T, Tanaka M, Nishimura S, Maekawa M, HBC study group
  • Journal Title: Medicine (Baltimore). Volume: 95(14) Issue: 14 Pages: e3219-e3219
  • DOI: 10.1097/md.0000000000003219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glycaemia and autistic traits in very low birth weight infants in adulthood. (2016)
  • Author(s): Sato R, Shirai K, Maekawa M, Genma R, Ohki S, Morita H, Suda T, Watanabe H
  • Journal Title: Diabetes Metab. Volume: 42(4) Issue: 4 Pages: 285-286
  • DOI: 10.1016/j.diabet.2016.02.005
  • Peer Reviewed / Open Access
• [Journal Article] ALP活性測定法の変更 - JSCC法からIFCC法へ (2016)
  • Author(s): 前川真人
  • Journal Title: 医学のあゆみ Volume: 259(8) Pages: 879-880
• [Journal Article] CGMを活かした血糖管理の有用性と今後の展望 (2016)
  • Author(s): 釣谷大輔、前川真人
  • Journal Title: 臨床病理 Volume: 64(10) Pages: 1171-1177
  • Peer Reviewed
• [Journal Article] 癌胎児性フィブロネクチン迅速定性試薬「ラビットチップR fFN」の基礎的検討 (2016)
  • Author(s): 金山尚裕、前川真人
  • Journal Title: 産科と婦人科 Volume: 83(5) Pages: 103-106
  • Peer Reviewed
• [Journal Article] Evaluation of the short-term stability of specimens for clinical laboratory testing. (2015)
  • Author(s): Ikeda K, Ichikawa K, Hashiguchi T, Hidaka Y, Kang D, Maekawa M, Matsumoto H, Matsushita K, Okubo S, Tsuchiya T, Furuta K
  • Journal Title: Biopreserv Biobank Volume: 13(2) Issue: 2 Pages: 135-143
  • DOI: 10.1089/bio.2014.0072
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Investigation of MDA-LDL (malondialdehyde-modified low-density lipoprotein) as a prognostic marker for coronary artery disease in patients with type 2 diabetes mellitus. (2015)
  • Author(s): Kotani K, Tashiro J, Yamazaki K, Nakamura Y, Miyazaki A, Bujo H, Saito Y, Kanno T, Maekawa M
  • Journal Title: Clin Chim Acta Volume: 450 Pages: 145-150
  • DOI: 10.1016/j.cca.2015.08.003
  • Peer Reviewed / Open Access
• [Journal Article] Pustular psoriasis-like lesions associated with hereditary lactate dehydrogenase M subunit deficiency without interleukin-36 receptor antagonist mutation: long-term follow-up of two cases. (2015)
  • Author(s): Ito T , Aoshima M, Sugiura K, Fujiyama T, Ito N, Sakabe JI, Akiyama M, Maekawa M, Tokura Y
  • Journal Title: Br J Dermatol. Volume: Epub Issue: 6 Pages: 1674-6
  • DOI: 10.1111/bjd.13590
  • Peer Reviewed / Open Access
• [Journal Article] Synergistic Proinflammatory Responses by IL-17A and Toll-Like Receptor 3 in Human Airway Epithelial Cells (2015)
  • Author(s): Mori K, Fujisawa T, Kusagaya H, Yamanaka K, Hashimoto D, Enomoto N, Inui N, Nakamura Y, Maekawa M, Suda T
  • Journal Title: PLoS One Volume: 10 Issue: 9 Pages: e0139491-e0139491
  • DOI: 10.1371/journal.pone.0139491
  • Peer Reviewed / Open Access
• [Journal Article] A case of treatment with voriconazole for chronic progressive pulmonary aspergillosis in a patient receiving tacrolimus for dermatomyositis-associated interstitial lung disease. (2015)
  • Author(s): Mochizuki E, Furuhashi K, Fujisawa T, Enomoto N, Inui N, Nakamura Y, Kono M, Hamada E, Maekawa M, Suda T
  • Journal Title: Respir Med Case Rep. Volume: 16 Pages: 163-165
  • DOI: 10.1016/j.rmcr.2015.10.008
  • Peer Reviewed / Open Access
• [Journal Article] Recommended use of Cut-off Folate Concentration in Serum and Erythrocyte(Red Blood Cell) as Expressed by Folic Acid Equivalent for the Diagnosis of Deficiency in Deliberating the Creation on Dietary Reference Intakes. (2015)
  • Author(s): Ihara H, Hirota K, Watanabe T, Totani M, Hashizume N, Aoki Y, Nagashima Y, Kamioka K, Onda K, Sunahara S, Suzuki T, Itabashi M, Ishibashi M, Ito S, Ohashi K, Ohta Y, Nobori T, Fujishiro K, Maekawa M, Miura M, Miyano H, Ando T, Nishimura K
  • Journal Title: Austin J Nutri Food Sci Volume: 3(1) Pages: 1055-1056
  • Peer Reviewed / Open Access
• [Journal Article] HbA1c NGSP値のサーベイ。 (2015)
  • Author(s): 村上正巳、柏木厚典、武井 泉、雨宮 伸、石橋みどり、植木浩二郎、佐藤麻子、永峰康孝、前川真人、矢冨 裕
  • Journal Title: 糖尿病 Volume: 58(1) Pages: 45-50
  • NAID: 130004905472
  • Peer Reviewed / Open Access
• [Journal Article] Final height and cardiometabolic outcomes in young adults with very low birth weight (< 1500 g). (2014)
  • Author(s): Sato R, Maekawa M, Genma R, Shirai K, Ohki S, Morita H, Suda T, Watanabe H
  • Journal Title: PLOS ONE Volume: 9 (11) Issue: 11 Pages: 1-9
  • DOI: 10.1371/journal.pone.0112286
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] National Institute of Standards and Technorogy SRM 972 as a reference material for serum total 25-hydroxyvitamin D measurements. (2014)
  • Author(s): Ihara H, Hirota K, Miura M, Kitajima I, Yamashita M, Nomura F, Nishimura M, Totani M, Hashizume N,Aoki Y, Nagashima Y, Kamioka K, Onda K, Sunahara S, Suzuki T, Itabashi M, Ishibashi M, Ito S, Ohashi K, Ohta Y, Nobori T, Fujishiro K, Maekawa M, Miyano H, Ando T, Nishimura K, Tsugawa N, Okano T
  • Journal Title: Int Anal Bio-Sci Volume: 2(1) Pages: 1-6
  • Peer Reviewed / Open Access
• [Journal Article] Plasma connective tissue growth factor levels as potential biomarkers of airway obstruction in patients with asthma. Ann Allergy Asthma Immunol. (2014)
  • Author(s): Kato M, Fujisawa T, Hashimoto D, Kono M, Enomoto N, Nakamura Y, Inui N, Hamada E, Miyazaki O, Kurashita S, Maekawa M, Suda T
  • Journal Title: Ann Allergy Asthma Immunol. Volume: 113 (3) Issue: 3 Pages: 295-300
  • DOI: 10.1016/j.anai.2014.05.026
  • Peer Reviewed / Open Access
• [Journal Article] Usual interstitial pneumonia preceding collagen vascular disease: a retrospective case control study of patients initially diagnosed with idiopathic pulmonary fibrosis. (2014)
  • Author(s): Kono M, Nakamura Y, Enomoto N, Hashimoto D, Fujisawa T, Inui N, Maekawa M, Suda T, Colby TV, Chida K
  • Journal Title: PLOS ONE Volume: 9 Issue: 4 Pages: e94775-e94775
  • DOI: 10.1371/journal.pone.0094775
  • Peer Reviewed / Open Access
• [Journal Article] IgG4-related skin disease. (2014)
  • Author(s): Tokura Y, Yagi H, Yanaguchi H, Majima Y, Kasuya A, Ito T, Maekawa M, Hashizume H
  • Journal Title: Br J Dermatol. Volume: 171 Issue: 5 Pages: 959-967
  • DOI: 10.1111/bjd.13296
  • Peer Reviewed / Open Access
• [Journal Article] Cell line differences in replication timing of human glutamate receptor genes and other large genes associated with neuraldisease. (2014)
  • Author(s): Watanabe Y, Shibata K, Maekawa M
  • Journal Title: Epigenetics Volume: 9 (10) Pages: 1350-1359
  • Peer Reviewed / Open Access
• [Journal Article] 臨床検査医学 アルブミン測定、BCP改良法とBCG法による測定値 (2014)
  • Author(s): 前川真人
  • Journal Title: 医学のあゆみ Volume: 251(2) Pages: 182-183
  • Open Access
• [Journal Article] 骨型ALP測定法。 (2014)
  • Author(s): 下田 綾、前川真人
  • Journal Title: 検査と技術 Volume: 42(13) Pages: 1382-1388
  • Open Access
• [Journal Article] 血清酵素検査で見出された異常データの電気泳動解析。 (2014)
  • Author(s): 前川真人
  • Journal Title: 臨床病理 Volume: 62(11) Pages: 1088-1093
  • Peer Reviewed / Open Access
• [Presentation] Th2サイトカインによる気道IL-17C発現抑制作用とその細胞内機序の解析 (2016)
  • Author(s): 山中勝正、藤澤朋幸、濱田悦子、須田隆文、前川真人
  • Organizer: 第56回日本臨床化学会年次学術集会
  • Place of Presentation: 熊本市
  • Year and Date: 2016-12-03
• [Presentation] サイトカインによる気道IL-17C発現制御の検討 (2016)
  • Author(s): 山中勝正、藤澤朋幸、濱田悦子、須田隆文、前川真人
  • Organizer: 第56回日本臨床化学会年次学術集会
  • Place of Presentation: 熊本市
  • Year and Date: 2016-12-03
• [Presentation] リゾルフィン基質を用いた新規リパーゼ活性測定試薬の基礎性能と他3種類の試薬との比較検討 (2016)
  • Author(s): 濱田悦子、前川真人
  • Organizer: 第56回日本臨床化学会年次学術集会
  • Place of Presentation: 熊本市
  • Year and Date: 2016-12-03
• [Presentation] 若年成人期に至った極低出生体重児（出生体重<1500g）におけるautism-spectrumスコアと血糖値の関連 (2016)
  • Author(s): 佐藤亮介、前川真人
  • Organizer: 第56回日本臨床化学会年次学術集会
  • Place of Presentation: 熊本市
  • Year and Date: 2016-12-03
• [Presentation] Trend of Turnaround Time in Our Laboratory. (2016)
  • Author(s): Hamada E, Kondo T, Maekawa M
  • Organizer: 14th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress
  • Place of Presentation: Taipei
  • Year and Date: 2016-11-27
  • Int'l Joint Research
• [Presentation] 蛋白分画電気泳動分析装置「Epalyzer2」の有用性評価 (2016)
  • Author(s): 山田将臣、下田 綾、濱田悦子、前川真人
  • Organizer: 第48回日本臨床検査自動化学会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-09-24
• [Presentation] がん患者数の推移と新規診断システム導入の臨床的意義 (2016)
  • Author(s): 前川真人
  • Organizer: 第14回医療機器フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2016-09-05
• [Presentation] IFCCとJSCCの活動とハーモナイゼーション (2016)
  • Author(s): 前川真人
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2016-09-03
• [Presentation] Ⅰ型糖尿病患者におけるSAP(Sensor Augmented Pump)の有用性の検討 (2016)
  • Author(s): 釣谷大輔 、濱田悦子 、河野雅人、森田浩 、沖隆 、前川真人
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2016-09-03
• [Presentation] 特発性肺線維症におけるPirfenidone血中濃度測定の検討 (2016)
  • Author(s): 河野雅人 、中村祐太郎 、片桐由起子、八木達也 、内藤隆文、釣谷大輔、濱田悦子、川上純一 、須田隆文、前川真人
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2016-09-03
• [Presentation] Study of reference materials suitable for the IFCC method of ALP measurement. (2016)
  • Author(s): Yamadate S , Yamazaki H , Matsusita M , Hoshino T, Ueda S, Maekawa M, Nakayama T
  • Organizer: 68th AACC Annual Scientific Meeting & Clinical Lab Expo
  • Place of Presentation: Philadelphia
  • Year and Date: 2016-08-03
• [Presentation] Basic Evaluation of a novel Glycohemoglobin Analyzer RC20 for POCT. (2016)
  • Author(s): Hamada E, Maekawa M
  • Organizer: 68th AACC Annual Scientific Meeting & Clinical Lab Expo
  • Place of Presentation: Philadelphia
  • Year and Date: 2016-08-03
• [Presentation] 臨床検査の標準化とハーモナイゼーション (2016)
  • Author(s): 前川真人
  • Organizer: 佐賀県臨床検査精度管理調査報告会
  • Place of Presentation: 佐賀
  • Year and Date: 2016-03-26
• [Presentation] 血清乳酸デヒドロゲナーゼ(LD)のアイソザイム異常 (2015)
  • Author(s): 前川真人
  • Organizer: 第55回日本臨床化学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-11-01
• [Presentation] Evaluation of “HISCL-TARC”, a Biomarker for Atopic Dermatitis, by Automated Immunoassay System “ HISCL 5000 ” (2015)
  • Author(s): Hamada E, Maekawa M
  • Organizer: 21st IFCC-EFLM European Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: Paris, France
  • Year and Date: 2015-06-22
  • Int'l Joint Research
• [Presentation] Mac-2 Binding Protein Glycosylation Isomer(M2BPGi) in Patients with Idopathic Pulmonary Fibrosis(IPF) (2015)
  • Author(s): Kono M, Nakamura Y, Oyama Y, Hozumi H, Karayama M, Hashimoto D, Enimoto N, Fujisawa T, Inui N, Hamada E, Maekawa M, Suda
  • Organizer: American Thoracic Society 2015 International Conference
  • Place of Presentation: Denver, USA
  • Year and Date: 2015-05-19
  • Int'l Joint Research
• [Presentation] Analytical evaluation of third-generation allergen-specific IgE assay “3gAllergyTM” Measurement by Automated Immunoassey System “IMMULITE 2000XPi”. (2014)
  • Author(s): Hamada E, Maekawa M
  • Organizer: AACC 2014 Annual Meeting& Clinical Lab Expo
  • Place of Presentation: McCormic Place Convention Center, Chicago USA
  • Year and Date: 2014-07-27 – 2014-07-31
• [Presentation] A case of prostate cancer producing granulocyte colony- stimulating factor. (2014)
  • Author(s): Hamada E,Takayama T, Ozono S, Maekawa M
  • Organizer: IFCC Worldlab Istanbul 2014
  • Place of Presentation: Istanbul Congress Center, Istanbul Turkey
  • Year and Date: 2014-06-22 – 2014-06-26
• [Book] 臨床検査データブックLABDATA2015-2016 (2015)
  • Author(s): 青沼和隆、青野敏博、秋山雄次、浅川明弘、阿部好文、天谷雅行、新井祐子、荒木拓也、荒木靖人、安藤雄一、安東由喜雄、飯高 誠、飯塚 誠、伊賀立二、五十嵐 隆、伊苅裕二、池森敦子、石井 彰、石井恵子、前川真人
  • Total Pages: 1059
  • Publisher: 医学書院
• [Book] 臨床検査ガイド2015改訂版 (2015)
  • Author(s): 福井次矢、斧 康雄、三嶋理晃、島田和幸、深澤 浩、奥新和也、小池和彦、廣田衛久、下瀬川 徹、五十嵐雅彦、丹治泰仁、野村 隆、三橋知明、工藤 工、伊藤 充、黒川峰夫、南谷泰仁、鈴木勝也、竹内 勤、前川真人
  • Total Pages: 1154
  • Publisher: 文光堂
• [Book] 引いて調べる先天代謝異常症 (2014)
  • Author(s): 青山友佳、市本景子、伊藤哲哉、伊藤 康、碓井ひろみ、大竹 明、大橋十哉、小川絢子、小国弘量、小沢 浩、折居建治、梶 俊策、加藤淳二、窪田 満、呉 繁夫、顧 艶紅、古賀靖敏、小須賀基道、児玉浩子、前川真人
  • Total Pages: 175
  • Publisher: 診断と治療社
• [Book] パーフェクトガイド検査値事典第2版 (2014)
  • Author(s): 高木 康、〆谷直人、野村文夫、澤部祐司、吉田俊彦、清宮正徳、梅村啓史、戸塚 実、日野田裕治、杉浦哲郎、高橋延行、橋本直明、光井 洋、池田 均、中川勇人、高橋伯夫、渡辺 毅、浅井 淳、飯野和美、前川真人
  • Total Pages: 707
  • Publisher: 総合医学社