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Establishment of evaluation method of drugs using a megakaryocytic cultured cells.

Research Project

Project/Area Number 26670282
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionThe University of Tokushima

Principal Investigator

HOSOI Eiji  徳島大学, 大学院医歯薬学研究部, 教授 (70229186)

Co-Investigator(Kenkyū-buntansha) AKI Kensaku  徳島大学, 大学院医歯薬学部研究部, 助教 (70646398)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords小板 / 巨核球系培養細胞 / Ca2+ / PMA / 分化誘導 / HEL / CMK / 薬剤評価法 / 血小板 / 巨核球系分化誘導 / 血小板モデル細胞
Outline of Final Research Achievements

In the present study, we attempted to improve the evaluation of various drugs on the platelet functions using PMA-induced megakaryocytic HEL cells and CMK cells as an alternative cell of platelets. We selected aspirin and cilostazol as antiplatelet drugs and ibuprofen and sodium valproate as other drugs. In conclusion, PMA-induced megakaryocytic HEL cells and CMK cells are a useful model for this study of platelet functions, and the quantification of inhibition of thrombin-induced [Ca2+]i and thrombin-induced CD62P antigen were applicable to the evaluation of various drugs on platelets. Furthermore, this evaluation system using these platelet model cells may contribute to appropriate drug selection and doses in order to control of the platelet functions without using the platelet, and is expected future use in clinical field.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Presentation (4 results)

  • [Presentation] 血小板モデル細胞を用いた各種薬剤併用における薬剤効果作用の評価2015

    • Author(s)
      酒井 美由希, 小林 沙綾, 多田 智紀, 大星 航, 植木 春香, 川添 和義, 安藝 健作, 細井 英司
    • Organizer
      第39回 徳島県医学検査学会
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2015-12-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血小板モデル細胞を用いた各種薬剤評価の検討2015

    • Author(s)
      多田 智紀, 安藝 健作, 大星 航, 川添 和義, 植木 春香, 川添 和義, 細井 英司
    • Organizer
      第10回 日本臨床検査学教育学会学術大会
    • Place of Presentation
      信州大学(長野県松本市)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 巨核球系培養細胞CMKを用いた抗血小板薬の薬剤評価法の検討2014

    • Author(s)
      原田 啓, 後藤 里香, 多田 智紀, 大星 航, 植木 春香, 川添 和義, 安藝 健作, 細井 英司
    • Organizer
      第38回 徳島県医学検査学会
    • Place of Presentation
      徳島大学保健学科C棟講義室(徳島県徳島市)
    • Year and Date
      2014-12-14
    • Related Report
      2014 Research-status Report
  • [Presentation] 巨核球系培養細胞を用いた血小板機能におよぼす薬剤評価法の検討2014

    • Author(s)
      多田 智紀, 大星 航, 安藝 健作, 細井 英司
    • Organizer
      2014 感染・免疫クラスター・ミニリトリート[徳島大学大学院ヘルスバイオサイエンス研究部主催]
    • Place of Presentation
      ルネッサンスリゾートナルト会議室(徳島県鳴門市)
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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