| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
We attempted to develop a new homogeneous chemiluminescent immunoassay using a combination of quinone labeled antibody and reactive oxygen species (ROS) scavenger labled antigen. The proposed assay is based on the chemiluminescence decrease due to the scavenging of ROS that is generated from quinone. After the screening of suitable compounds for labeling, anthraquinone (AQ) and tyramine (TA) were selected as quinone and ROS scavenger, respectively. Then, we synthesized a model compound (AQ-TA) that contains AQ and TA moieties in one molecule in order to evaluate the interaction between quinone and ROS scavenger. As we expected, the chemiluminescence intensity of AQ-TA was weaker than that of control compound that does not contain TA moiety. Therefore, the possibility of development of the proposed homogeneous immunoassay was sufficiently suggested.
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