Optogenetic manipulation of spinal local circuit which modulate pain/itch transmission
| Project/Area Number |
26670289
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Kagoshima University (2016)
Saga University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
園畑 素樹 佐賀大学, 医学部, 准教授 (50304895)
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| Research Collaborator |
Todd Andrew J グラスゴー大学, 教授
Hughes David I グラスゴー大学, 講師
Robert Callister ニューカッスル大学, 教授
Graham Brett A ニューカッスル大学, 准教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 疼痛学 / 痒み / オプトジェネティクス / 脊髄後角 / 局所回路 / オプトジェネティクス（光遺伝学） / 局所神経回路 |
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| Outline of Final Research Achievements |
To elucidate a mechanism of intractable pain is urgent. The spinal dorsal horn is a target of primary afferents which convey sensory information (include pain). These inputs are processed and modified by local circuits which consist of interneurons. These interneurons are heterogeneous and little is known about connectivity among them. To clarify roles of each type of interneurons, we planned to employ an optogenetic approach. We chose a parvalbumin (PV) expressing population as one of interneurons. Among setting-up an experimental environment for optogenetics, a combined electrophysiological and anatomical approach was used to investigate role of PV interneurons. We found that tactile inputs to the vertical cell were likely suppressed by PV interneurons. The vertical cell is a class of excitatory interneuron possessing axons which innervate lamina I projection neurons. Thus, these result suggested that PV cells were important to supress the pathway involving tactile allodynia.
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Report
(4 results)
Research Products
(21 results)
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[Presentation] Possible Contribution Of The C-Type Lectin Receptor, Mincle, To Induction Of Neuropathic Pain After Peripheral Nerve Injury In Mice2016
Author(s)
Ishikawa A, Yasaka T, Miyake Y, Murata Y, Sasaguri T, Yamasaki S, Hara H, Yoshida H, Hirakawa N
Organizer
The 16th World Congress on Pain
Place of Presentation
パシフィコ横浜（神奈川県・横浜市）
Year and Date
2016-09-26
Related Report
Int'l Joint Research
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[Presentation] 脊髄疼痛誘導サイトカインにはC型レクチンが必要である2016
Author(s)
石川亜佐子、八坂敏一、笹栗智子、平川奈緒美、村田祐造、三宅靖延、山崎晶、原博満、吉田裕樹
Organizer
第81回インターフェロン・サイトカイン学会学術集会
Place of Presentation
長崎大学坂本キャンパス良順会館・ポンペ会館（長崎県・長崎市）
Year and Date
2016-05-13
Related Report
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[Presentation] 痛み行動におけるIL-27の役割2016
Author(s)
笹栗智子、八坂敏一、石川亜佐子、村田祐造、原博満、平川奈緒美、吉田裕樹
Organizer
第81回インターフェロン・サイトカイン学会学術集会
Place of Presentation
長崎大学坂本キャンパス良順会館・ポンペ会館（長崎県・長崎市）
Year and Date
2016-05-13
Related Report
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[Presentation] パルブアルブミンニューロンによる低閾値機械受容線維終末の制御2014
Author(s)
八坂 敏一, Boyle K, Shehab SA, Scott DT, Riddell JS, 藤田 亜美, 熊本 栄一, Callister RJ, Graham BA, Hughes DI
Organizer
平成26年度生理学研究所研究会 痛みと痛覚情動連関の神経機構
Place of Presentation
岡崎
Year and Date
2014-12-10 – 2014-12-11
Related Report
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