| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
High pressure supersaturated water was produced and ultrasound was irradiated. As a result, very small microsized bubble formed within the liquid. Water pressurized to 0.5MPa in combination with air was made to a supersaturated water condition. The water was added to HL-60 leukemia cell in combination with PI staining agent and trypan blue stain. After ultrasound exposure, the cell showed uptake of the two stain which suggests that the microsized bubble contributed to this phenomenon. It was suggested that ultrasound delivered drugs within the cell without cell killing. The microbubbles also showed high acoustic signals using conventional ultrasound diagnostic device. Supersaturated water might be useful as a ultrasound contrast agent as well as drug delivery agent.
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