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Paleopathological genetic analysis to unveil national history of tuberculosis in Japan and its application to molecular epidemiology

Research Project

Project/Area Number 26670332
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Hygiene and public health
Research InstitutionNagasaki University

Principal Investigator

WADA Takayuki  長崎大学, 熱帯医学研究所, 助教 (70332450)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Shiomi  独立行政法人国立病院機構(近畿中央胸部疾患センター臨床研究センター), 感染症研究部, 流動研究員 (40260806)
Co-Investigator(Renkei-kenkyūsha) KITAICHI Masanori  独立行政法人国立病院機構(近畿中央胸部疾患センター臨床研究センター), 臨床検査部, 臨床検査部長 (00161464)
NAGAI Hideaki  独立行政法人国立病院機構(東京病院), 臨床研究部, 外来診療部長 (30510391)
HEBISAWA Akira  独立行政法人国立病院機構(東京病院), 臨床研究部, 臨床研究部長 (70549146)
ICHIKAWA Tomoo  長崎大学, 熱帯医学研究所, 助教 (30508875)
MAEDA Shinji  公益財団法人結核予防会結核研究所, 抗酸菌情報科, 科長 (50250212)
Research Collaborator HANASHIMA Makoto  国立研究開発法人防災科学技術研究所, 主幹研究員
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords分子疫学 / 感染症 / 古病理学 / 歴史学 / ゲノム
Outline of Final Research Achievements

Clinical samples such as paraffin blocks for histopathological diagnosis of tuberculosis about several decades ago (1960s to 1970s) were challenged to purify DNA of the etiological agent, Mycobacterium tuberculosis. Phylogenetic classification of these samples reveals that unique population genetic structure of M. tuberculosis in Japan has been already seen. A genetic group B2, which was considered to have spread for several hundred years from genomic comparative analysis, was also found frequently in the old samples. The DNA analysis techniques to target on mycobacterial DNA are applicable to clinical diagnosis for mycobacterial infection such as extra pulmonary tuberculosis and veterinary cases.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] A tumor in chest wall caused by Mycobacterium bovis BCG infection.2016

    • Author(s)
      Yokoyama K, Kubo K, Yoshida S, Wada T, Kawai T, Nishikomori R, Yoshida A.
    • Journal Title

      Pediatrics International

      Volume: 58(4) Issue: 4 Pages: 317-318

    • DOI

      10.1111/ped.12894

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of Mycolic Acids in Total Fatty Acid Methyl Ester Fractions from <i>Mycobacterium</i> Species by High Resolution MALDI-TOFMS2015

    • Author(s)
      Teramoto K, Suga M, Sato T, Wada T, Yamamoto A, Fujiwara N
    • Journal Title

      Mass Spectrometry

      Volume: 4 Issue: 1 Pages: A0035-A0035

    • DOI

      10.5702/massspectrometry.A0035

    • NAID

      130005095680

    • ISSN
      2186-5116, 2187-137X
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Primary gingival tuberculosis diagnosed by genetic identification2015

    • Author(s)
      Okano Y, Yoshida S, Shinohara T, Takahashi N, Naito N, Kagawa K, Machida H, Hatakeyama N, Ohno A, Wada T, Ogushi F.
    • Journal Title

      Internal Medicine

      Volume: 54

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Uniqueness of Clinical Strains of Mycobacterium tuberculosis in Japan: A Clue to Elucidate the History of Transmission and Diffusion of Tuberculosis.2015

    • Author(s)
      Takayuki Wada
    • Organizer
      The Third Conference of East Asian Environmental History (EAEH 2015)
    • Place of Presentation
      香川大学(香川県・高松市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tuberculosis History from the Relationship between Cattle and Human: Mycobacterium bovis and Modern Japan.2015

    • Author(s)
      Tomoo Ichikawa
    • Organizer
      The Third Conference of East Asian Environmental History (EAEH 2015)
    • Place of Presentation
      香川大学(香川県・高松市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Revisiting “Joko and Kekkaku” :A Socio-Economic Approach to Decipher the Relationship between Industrialization and Tuberculosis in Modern Japan.2015

    • Author(s)
      Makoto Hanashima
    • Organizer
      The Third Conference of East Asian Environmental History (EAEH 2015)
    • Place of Presentation
      香川大学(香川県・高松市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゲノム解析が解き明かす結核の拡散経緯とこれから2015

    • Author(s)
      和田 崇之
    • Organizer
      第20回国際結核セミナー
    • Place of Presentation
      ヤクルトホール(東京都港区)
    • Year and Date
      2015-03-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 1960~70年代における結核菌の薬剤耐性状況の推察と系統進化2014

    • Author(s)
      吉田志緒美、露口一成、鈴木克洋
    • Organizer
      第62回日本化学療法学会西日本支部総会・第57回日本感染症学会中日本地方会学術集会・第84回日本感染症学会西日本地方会学術集会合同集会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
    • Year and Date
      2014-10-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Opportunistic and pathogenic Mycobacteria: importance of systematic surveillance of mycobacteriosis based on pathological diagnosis2014

    • Author(s)
      Takayuki Wada, Shiomi Yoshida, Taro Yamamoto, Tokuma Yanai
    • Organizer
      Asian Society of Zoo and Wildlife Medicine (2014 meeting in Vietnam)
    • Place of Presentation
      Tam Dao National Park (Tam Dao, Vietnam)
    • Year and Date
      2014-10-14
    • Related Report
      2014 Research-status Report
  • [Presentation] 1960 ~ 70 年代における結核菌の薬剤耐性状況の推察ー抗結核薬の変遷とその影響について2014

    • Author(s)
      吉田志緒美、露口一成、岡田全司、鈴木克洋、林清二、富田元久、和田崇之、山本太郎
    • Organizer
      第89回日本結核病学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2014-05-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 結核菌国内臨床株の比較ゲノム解析:大規模拡散株と国内定着系統群を追跡する2014

    • Author(s)
      和田 崇之
    • Organizer
      第84回実験結核研究会
    • Place of Presentation
      長良川国際会議場(岐阜)
    • Year and Date
      2014-05-08
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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