| Project/Area Number |
26670350
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Medical and hospital managemen
|
|---|
| Research Institution | International University of Health and Welfare |
|---|
Principal Investigator |
NAGASAWA ZENZO 国際医療福祉大学, 保健医療学部, 教授 (80706820)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 疫学解析 / MRSA / 緑膿菌 / MALDI-TOF MS / PFGE法 / POT法 / REP-PCR法 / リボゾーム蛋白 / Clostridium difficile / MALDI TOF-MS / PFGE / POT |
|---|
| Outline of Final Research Achievements |
MALDI Biotyper 3.1 and ClinPro Tools ver.2.2 were used and measurement data for MRSA, Pseudomonas aeruginosa and Clostridium difficile was analyzed in this study. We could originally develop novel test method(IUHW method) using MALDI-TOF MS and clone typing data analysis with epidemiological auto-classification Good relationship with IUHW method was obtained between PFGE, POT, REP-PCR for MRSA. But relationship discrepancy with IUHW method was observed in some clones for P. aeruginosa. Cluster classification was not achieved for C. difficile between PCR-ribotyping and ClinPro Tools. We think it is possible to achieve rapid classification of clones using MALDI-TOF MS by development of optimal measurement conditions and continuous study has been conducted.
|
