Postmortem diagnosis of arrhythmic death by using iPS cells

• Project/Area Number: 26670355
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Osaka University
• Principal Investigator: Matsumoto Hiroshi 大阪大学, 医学(系)研究科(研究院), 教授 (60263092)
• Research Collaborator: 杉本 香奈 ; Yang Chi-pin ; 平田 雄一郎 ; 鎌倉 悠
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 突然死 / 機能性疾患 / iPS / 死因診断 / iPS cells / postmortem diagnosis / functional disease / arrythmia / mice / iPS細胞 / 不整脈 / 心筋細胞 / 死因究明

Outline of Final Research Achievements

Arrhythmia has never been diagnosed after death. Then, we hypothesized that arrhythmia can be postmortem diagnosed iiPS-related cardiomyocyte induced by skin fibroblast. To clarify the hypothesis, the object in this study was to found iPS cell culture and established the system for detection of arrhythmia using carddiomyocytes. It was difficult to culture iPS cells, leading to increase fibroblasts. On there other hand, we could detect arrhythmia in cardipmyocytes using MED64. We are challenging the isolation of skin fibroblast after death and inducing iPS cells, leading to postmortem diagnosis of arrhythmia using iPS cells.

Research Products

• [Remarks] iPS細胞を用いた致死的不整脈の死後診断の試み
  • URL: http://www.legal.med.osaka-u.ac.jp/