| Project/Area Number |
26670357
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
Ishida Yuko 和歌山県立医科大学, 医学部, 講師 (10364077)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Akihiko 和歌山県立医科大学, 医学部, 准教授 (60136611)
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| Research Collaborator |
KUNINAKA Yumi
YAMAMOTO Hiroki
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 創傷治癒 / 法医病理学 / オートファジー |
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| Outline of Final Research Achievements |
The dorsal skin of Balb/c wild-type mice were wounded with a diameter of 4 mm biopsy punch. Gene and protein expressions of LC3 were significantly reduced at 1 day after the injury compared with unwounded skin. On the other hand, gene and protein expressions of p62 were significantly increased at 1 day after the injury compared with unwounded skin. Moreover, protein expression of LC3 and p62 were found in F4/80-positive macrophages on the tissue sections. Collectively, macrophages might be activated after injury along with autophagy inhibition, therefore, autophagy detection can be a useful marker for wound age determination.
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