Detection of autophagy in human skin wounds and its application for wound age detemination

• Project/Area Number: 26670357
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: Ishida Yuko 和歌山県立医科大学, 医学部, 講師 (10364077)
• Co-Investigator(Kenkyū-buntansha): KIMURA Akihiko 和歌山県立医科大学, 医学部, 准教授 (60136611)
• Research Collaborator: KUNINAKA Yumi ; YAMAMOTO Hiroki
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 創傷治癒 / 法医病理学 / オートファジー

Outline of Final Research Achievements

The dorsal skin of Balb/c wild-type mice were wounded with a diameter of 4 mm biopsy punch. Gene and protein expressions of LC3 were significantly reduced at 1 day after the injury compared with unwounded skin. On the other hand, gene and protein expressions of p62 were significantly increased at 1 day after the injury compared with unwounded skin. Moreover, protein expression of LC3 and p62 were found in F4/80-positive macrophages on the tissue sections. Collectively, macrophages might be activated after injury along with autophagy inhibition, therefore, autophagy detection can be a useful marker for wound age determination.

Research Products

• [Journal Article] Autophagy in skin wounds: a novel marker for vital reactions (2015)
  • Author(s): Kimura A, Ishida Y, Nosaka M, Shiraki M, Hama M, Kawaguchi T, Kuninaka Y, Shimada E, Yamamoto H, Takayasu T, Kondo T.
  • Journal Title: Int J Legal Med Volume: - Issue: 3 Pages: 537-541
  • DOI: 10.1007/s00414-015-1168-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination (2015)
  • Author(s): Ishida Y, Kuninaka Y, Nosaka M, Kimura A, Kawaguchi T, Hama M, Sakamoto S, Shinozaki K, Eisenmenger W, Kondo T.
  • Journal Title: Int J Legal Med Volume: - Issue: 5 Pages: 1043-1048
  • DOI: 10.1007/s00414-015-1167-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination. (2015)
  • Author(s): Ishida Y, Kimura A, Nosaka M, Kuninaka Y, Shimada E, Yamamoto H, Nishiyama K, Inaka S, Takayasu T, Eisenmenger W, Kondo T.
  • Journal Title: Int J Legal Med. Volume: 129(5) Issue: 5 Pages: 1049-54
  • DOI: 10.1007/s00414-015-1181-7
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination． (2015)
  • Author(s): 石田裕子
  • Organizer: 第99次日本法医学会学術全国集会
  • Place of Presentation: 高知市
  • Year and Date: 2015-06-10
• [Presentation] マウス皮膚創傷治癒におけるオートファジーの役割解析－その１－ (2014)
  • Author(s): 近藤稔和
  • Organizer: 第44回日本創傷治癒学会
  • Place of Presentation: 仙台
  • Year and Date: 2014-12-03