An experimental trial to determine the presence and absence of antemortem seizure using nuclear magnetic resonance analysis of rat postmortem cerebrospinal fluid
| Project/Area Number |
26670359
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Kaoru 京都大学, 医学（系）研究科(研究院), 教授 (10267164)
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| Co-Investigator(Renkei-kenkyūsha) |
KANAWAKU Yoshimasa 日本医科大学, 医学（系）研究科(研究院), 講師 (80465343)
MORIYAMA Tsuyoshi 東京工芸大学, 工学部, 准教授 (80449032)
OHNO Youkichi 日本医科大学, 医学（系）研究科(研究院), 教授 (70152220)
KANETAKE Jun 防衛医科大学校（医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科専門課程, 教授 (90326661)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | けいれん / 死後髄液 / 核磁気共鳴法 / パターン認識 / 死体検案 |
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| Outline of Final Research Achievements |
We performed nuclear magnetic resonance (NMR) analysis for rat postmortem cerebrospinal fluid (CSF) and ascertained if presence and absence of antemortem seizure could be detected. Male Sprague-Dawley rats (9 weeks, 220 g) were randomized into four groups. 1) seizure group (n=13); saline (12mL/kg) ip + pentylentetrazole (PTZ) (70 mg/kg) ip 6 hrs later, 2) seizure suppression group (n=10); pentobarbital (PB) (90mg/kg) ip + PTZ ip 6 hrs later, 3) anticonvulsant alone group (n=13); PB ip + saline (1.75mL/kg) ip 6 hrs later, 4) control group (n=9); saline + saline ip 6 hrs later. After animals were euthanized by CO2 gas, CSF samples collected in the cisternal compartment were analyzed by proton NMR spectroscopy. The NMR spectral profile of 4 groups showed separation and clustering on a PCA score plot.
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Report
(2 results)
Research Products
(1 results)
