| Project/Area Number |
26670360
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
ISOBE Ichiro 藤田保健衛生大学, 医学部, 教授 (30315907)
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| Co-Investigator(Renkei-kenkyūsha) |
OCHI Hiroshi 藤田保健衛生大学, 医学部, 助教 (70527704)
HIRATA Yukari 藤田保健衛生大学, 医学部, 助教 (50156676)
HAMAJIMA Makoto 藤田保健衛生大学, 医学部, 助教 (20189608)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 脂肪細胞 / 心筋細胞 / 内因性カンナビノイドシステム / 薬物 / 内因性カンナビノイド / 低温傷害 |
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| Outline of Final Research Achievements |
Our purpose was to investigate the roles of adipocyte on the pathophysiology of the cardiovascular system and to clarify the mechanisms underlying serious pathological outcome including sudden death and to find out biomarkers that indicate abnormal conditions of adipocyte. To achieve our purpose, we tried to prepare a culture system model of adipocyte using 3T3-L1 cell line. This cell line has been utilized widely as an adipocyte model, but the 3T3-L1 cell have originally fibroblast-like phenotype and have to be differentiated into adipocyte-like phenotype by a couple of reagents. Moreover, it is difficult to maintain the adipocyte-like phenotype for a long time in a culture system. We, therefore, tested various conditions of cell culture, and we recognized that the number of cell passage, cell confluency, or timing of addition or removal of differentiation reagents were important factors for establishing a stable culture system.
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