Search and functional analysis of secreted microRNA associated with mitochondrial quality control

• Project/Area Number: 26670374
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: Department of Clinical Research, National Hospital Organization Tokushima National Hospital
• Principal Investigator: Mitsui Takao 独立行政法人国立病院機構徳島病院(臨床研究部), その他部局等, その他 (80294726)
• Co-Investigator(Kenkyū-buntansha): MAKI YUKIKO 独立行政法人国立病院機構・徳島病院, 臨床研究部, 研究員 (70398014) ; KAWAMURA KAZUYUKI 独立行政法人国立病院機構・南京都病院, 臨床研究部, 臨床研究部長 (10450959)
• Co-Investigator(Renkei-kenkyūsha): KAJI RYUJI 徳島大学, 大学院臨床神経科学, 教授 (00214304)
• Research Collaborator: TAKAHASHI RYOSUKE ; FUJIMOTO MIKI ; SOGO MASAKO
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: パーキン / miRNA / microRNA / 分泌型 / ミトファジー / ミトコンドリア / 品質管理

Outline of Final Research Achievements

We have reported that the Parkin protein to promote transcription of mitochondrial genes.We hypothesized that Parkin is involved in the processing of miRNA associated with the mitochondria.In the present study, we investigated the role of Parkin in miRNA processing. We found that parkin is bonded to Drosha protein in the nuclear and mitochondrial fractions of cultured cells. This result suggests that Parkin to the involved miRNA processing via the Drosha complex in the nuclei and mitochondria. We have found that Parkin is bonded to miR132 and pre-miR132 in both fractions.

Research Products

• [Journal Article] Gene analysis of Parkinson's disease in Tokushima National Hospital (2016)
  • Author(s): Y Maki, M Fujimoto, K Nagahama, T Mitsui
  • Journal Title: J Tokushima Natl Hosp Volume: 7 Pages: 4-7
  • NAID: 40020914735
  • Peer Reviewed
• [Journal Article] Adductor laryngeal exhaling dystonia in progressive supranuclear palsy (2015)
  • Author(s): Kawamura K, Arii Y, Inui T, Mitsui T
  • Journal Title: Neurology Volume: 84(5) Pages: 545-545
  • Peer Reviewed
• [Journal Article] A homozygous mutation of VWA3B causes cerebellar ataxia with intellectual disability (2015)
  • Author(s): Kawarai T, Tajima A, Kuroda Y, Saji N, Orlacchio A, Terasawa H, Shimizu H, Kita Y, Izumi Y, Mitsui T, Imoto I, Kaji R
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: 8 Pages: 309828-309828
  • Peer Reviewed
• [Journal Article] Adductor laryngeal exhaling dystonia in progressive supranuclear palsy (2015)
  • Author(s): Kawamura K, Arii Y, Inui T, and Mitsui T
  • Journal Title: Neurology Volume: 84 Issue: 5 Pages: 545-545
  • DOI: 10.1212/wnl.0000000000001206
  • Peer Reviewed
• [Journal Article] Immediate effect of spinal magnetic stimulation on camptocormia in Parkinson's disease. (2014)
  • Author(s): Arii Y, Sawada Y, Kawamura K, Miyake S, Taichi Y, Izumi Y, Kuroda Y, Inui T, Kaji R, Mitsui T.
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: 未定 Issue: 11 Pages: 1221-1226
  • DOI: 10.1136/jnnp-2014-307651
  • Peer Reviewed
• [Journal Article] Superoxide dismutase as a target of clioquinol-induced neurotoxicity. (2014)
  • Author(s): Kawamura K, Kuroda Y, Sogo M, Fujimoto M, Inui T, Mitsui T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 未定 Issue: 1 Pages: 181-5
  • DOI: 10.1016/j.bbrc.2014.04.067
  • Peer Reviewed
• [Presentation] Parkin is involved in miR132 processing (2016)
  • Author(s): 三ツ井 貴夫
  • Organizer: 第57回日本神経学会学術大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2016-05-18
• [Presentation] 呼気時に限定した喉頭ジストニアを呈した進行性核上性麻痺の一例 (2014)
  • Author(s): 川村 和之
  • Organizer: 徳島神経病学学術講演会
  • Place of Presentation: ホテルグランドパレス徳島
  • Year and Date: 2014-10-10
• [Presentation] 近年の潮流である「持続性ドパミン刺激（CDS）について (2014)
  • Author(s): 三ツ井 貴夫
  • Organizer: 大塚製薬学術講演会
  • Place of Presentation: ホテルクレメント徳島
  • Year and Date: 2014-10-07
• [Presentation] パーキンソン関連疾患とリハビリテーション (2014)
  • Author(s): 三ツ井 貴夫
  • Organizer: 堺市医療講演会
  • Place of Presentation: 堺市健康福祉プラザ
  • Year and Date: 2014-09-27
• [Presentation] HLA-DRトランスジェニックマウスを用いた多発性硬化症の病態解析 (2014)
  • Author(s): 川村 和之
  • Organizer: 京都大学大学院医学研究科 脳病態生理学講座 臨床神経学
  • Place of Presentation: 京都大学医学部付属病院
  • Year and Date: 2014-09-26
• [Presentation] パーキンソン病の治療と問題点 (2014)
  • Author(s): 三ツ井 貴夫
  • Organizer: 阿波吉野川支部・名西支部薬剤師会
  • Place of Presentation: セントラルホテル鴨島
  • Year and Date: 2014-09-16