| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
This study aimed to establish intrahepatic biliary epithelial cells by direc reprogramming method. Briefly, human fibroblast cells were hervested from oral cavity, and cultured. Thereafter, several genes were introduced using retroviral vector. After adequate screeming, cells were evaluated their phenotype, physiologically, biochemically, and molecularly. Our established cells exhibited amylase production, which is regarded as the marker of pancreatic ductal cells. However, our cells did not exhibited the markers as the intrahepatic biliary epithelial cells such as cytokerating 7, bicarbonate production, and expression of biliary transporters. Thus, up to now, our study did not achieve the establishment of aimed cells. Given the fact that pancreatic ductal cells is a similar cells to bile duct cells, further improvement could enables us to develop aimed cells.
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