Development of an ultra-early detection method for recurrence of hepatocellular carcinoma using a microcavity array liquid biopsy system
Project/Area Number |
26670379
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | University of Fukui |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 癌 / 細胞・組織 / 蛋白 / ゲノム / 内科 |
Outline of Final Research Achievements |
A method for efficient enrichment of circulating tumor cells (CTCs) was evaluated in the blood of patients with hepatocellular carcinoma (HCC) using a microcavity array system. When HCC cell lines HepG2, Huh7 and PLC/PRF/5 were mixed in heathy blood, the detection rates were 65%, 77% and 99%, respectively. In 3mL blood of 19 HCC patients, 8.8±8.7 and 125.0±176.3 of CTCs were isolated in stages I-III and IV (P<0.05), respectively, indicating that the numbers of CTCs may be correlated with the progression of HCC. The results demonstrate the establishment of a novel liquid biopsy method for CTCs in HCC and suggest its clinical significance on the disease development.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Identification of novel variants in HLA class II region related to HLA DPB1 expression and disease progression in patients with chronic hepatitis C.2017
Author(s)
Hiramatsu K, Matsuda H, Nemoto T, Nosaka T, Saito Y, Naito T, Takahashi K,Ofuji K, Ohtani M, Suto H, Yasuda T, Hida Y, Kimura H, Soya Y, Nakamoto Y.
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Journal Title
J Med Virol.
Volume: in press
Issue: 9
Pages: 1574-1583
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Perioperative plasma glypican-3 level may enable prediction of the risk of recurrence after surgery in patients with stage I hepatocellular carcinoma.2017
Author(s)
Ofuji K, Saito K, Suzuki S, Shimomura M, Shirakawa H, Nobuoka D, Sawada Y, Yoshimura M, Tsuchiya N, Takahashi M, Yoshikawa T, Tada Y, Konishi M, Takahashi S, Gotohda N, Nakamoto Y, Nakatsura T.
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Journal Title
Oncotarget.
Volume: in press
Issue: 23
Pages: 37835-37844
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long-Term Endoscopic Follow-Up of Patients with Chronic Radiation Proctopathy after Brachytherapy for Prostate Cancer2016
Author(s)
M. Ohtani, H. Suto, T. Nosaka, Y. Saito, Y. Ozaki, R. Hayama, T. Naito, K. Takahashi, K. Ofuji, H. Matsuda, K. Hiramatsu, T. Nemoto, H. Shioura, H. Kimura, Y. Aoki, O. Yokoyama, Y. Nakamoto
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Journal Title
Diagn Ther Endosc
Volume: 2016:1414090
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Preoperative diagnosis of cavernous hemangioma presenting with melena using wireless capsule endoscopy of the small intestine.2016
Author(s)
Akazawa Y, Hiramatsu K, Nosaka T, Saito Y, Ozaki Y, Takahashi K, Naito T, Ofuji K, Matsuda H, Ohtani M, Nemoto T, Suto H, Yamaguchi A, Imamura Y, Nakamoto Y.
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Journal Title
Endosc. Int. Open
Volume: 4
Issue: 03
Pages: E249-E251
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] High-dose cyclophosphamide induces specific tumor immunity with concomitant recruitment of LAMP1/CD107a-expressing CD4-positive T cells into tumor sites.2015
Author(s)
Naito, T., Baba, T., Takeda, K., Sasaki, S., Nakamoto, Y. and Mukaida, N.
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Journal Title
Cancer Letters
Volume: 366
Issue: 1
Pages: 93-99
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Pituitary metastasis of hepatocellular carcinoma presenting with panhypopituitarism: a case report.2015
Author(s)
Tanaka T, Hiramatsu K, Nosaka T, Saito Y, Naito T, Takahashi K, Ofuji K, Matsuda H, Ohtani M, Nemoto T, Suto H, Yamamoto T, Kimura H, Nakamoto Y.
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Journal Title
BMC Cancer
Volume: 15
Issue: 1
Pages: 863-863
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ruptured hepatic metastases of cutaneous melanoma during treatment with vemurafenib: an autopsy case report.2015
Author(s)
Nosaka T, Hiramatsu K, Nemoto T, Saito Y, Ozaki Y, Takahashi K, Naito T, Ofuji K, Matsuda H, Ohtani M, Suto H, Imamura Y, Nakamoto Y.
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Journal Title
BMC Clin. Pathol.
Volume: 15
Issue: 1
Pages: 15-15
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A case of Helicobacter pylori-negative intramucosal well-differentiated gastric adenocarcinoma with intestinal phenotype.2015
Author(s)
Ozaki Y, Suto H, Nosaka T, Saito Y, Naito T, Takahashi K, Ofuji K, Matsuda H, Ohtani M, Hiramatsu K, Nemoto T, Imamura Y, Nakamoto Y.
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Journal Title
Clin J Gastroenterol
Volume: 8
Issue: 1
Pages: 18-21
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In vivo immunological antitumor effect of OK-432-stimulated dendritic cell transfer after radiofrequency ablation.2014
Author(s)
Nakagawa H, Mizukoshi E, Iida N, Terashima T, Kitahara M, Marukawa Y, KitamuraK, Nakamoto Y, Hiroishi K, Imawari M, Kaneko S
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Journal Title
Cancer Immunol Immunother.
Volume: 63(4)
Issue: 4
Pages: 347-356
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparison of hepatic arterial infusion chemotherapy and sorafenib in elderly patients with advanced hepatocellular carcinoma: A case series.2014
Author(s)
Nemoto T, Matsuda H, Nosaka T, Saito Y, Ozaki Y, Hayama R, Naito T, Takahashi K, Ofuji K, Ohtani M, Hiramatsu K, Suto H, Nakamoto Y
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Journal Title
Mol. Clin. Oncol.
Volume: 2 (6)
Pages: 1028-1034
Related Report
Peer Reviewed / Open Access
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[Presentation] Perioperative plasma glypican-3 levels predict the risk of post-operative recurrence.2016
Author(s)
Ofuji K, Saito K, Nosaka T, Takahashi K, Naito T, Matsuda H, Ohtani M, Hiramatsu K, Nemoto T, Nakamoto Y.
Organizer
The 67th Annual Meeting of the American Association for the Study of Liver Diseases (AASLD 2016)
Place of Presentation
Boston, Massachusetts
Year and Date
2016-11-11
Related Report
Int'l Joint Research
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[Presentation] Influence of antithrombotic drugs on gastric ulcer healing after endoscopic submucosal dissection.2016
Author(s)
Ohtani M, Suto H, Nosaka T, Saito Y, Ozaki Y, Hayama R, Naito T, Takahashi K, Ofuji K, Matsuda H, Hiramatsu K, Nemoto T, Nakamoto Y.
Organizer
Digestive Disease Week 2016
Place of Presentation
San Diego U.S.A.
Year and Date
2016-05-21
Related Report
Int'l Joint Research
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[Presentation] Endoscopic long-term follow-up of chronic radiation proctopathy after brachytherapy for prostate cancer.2016
Author(s)
Takahashi K, Suto H, Nosaka T, Saito Y, Ozaki Y, Hayama R, Naito T, Ohtani M, Ofuji K, Matsuda H, Hiramatsu K, Nemoto T, Nakamoto Y.
Organizer
Digestive Disease Week 2016
Place of Presentation
San Diego U.S.A.
Year and Date
2016-05-21
Related Report
Int'l Joint Research
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