• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Significance of connective tissue growth factor (CTGF) and tumor-stroma interaction in Kras-driven hepatocarcinogenesis and HCC progression

Research Project

Project/Area Number 26670381
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

Takehara Tetsuo  大阪大学, 医学(系)研究科(研究院), 教授 (70335355)

Co-Investigator(Kenkyū-buntansha) Hikita Hayato  大阪大学, 大学院医学系研究科, 助教 (20623044)
巽 智秀  大阪大学, 医学(系)研究科(研究院), 講師 (20397699)
Co-Investigator(Renkei-kenkyūsha) Makino Yuki  大阪大学, 大学院医学系研究科, 医員 (60771334)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsKras / CTGF / 発癌 / 肝発癌
Outline of Final Research Achievements

Connective tissue growth factor (CTGF) was found to be up-regulated in tumor area in a Kras-mutated HCC mouse model by the activation of Ras/Mek/Erk pathway. Hepatocyte-specific knockout of CTGF in this mouse model revealed significant decrease of tumor diameter, number, and liver/body weight ratio and better tumor differentiation degrees. In surgically resected human HCC, CTGF was up-regulated in tumor area in the same way as Kras-mutated HCC mouse model and CTGF expression levels were shown to be associated with the malignant grade of HCC. Although forced expression of CTGF in HCC cell lines did not affect the in vitro cell viability or tumor volume of xenograft models, they significantly increased in CTGF-overexpressing HCC cell lines in the coexistence of hepatic stellate cells. From these results, CTGF was proven to be the molecule that promotes HCC progression via tumor-stroma interaction between HCC cells and hepatic stellate cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Presentation (9 results) (of which Int'l Joint Research: 3 results)

  • [Presentation] Significance of the up-regulation of connective tissue growth factor in hepatocellular carcinoma2016

    • Author(s)
      Yuki Makino, Hayato Hikita, Takahiro Kodama, Minoru Shigekawa, Tasuku Nakabori, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Tomohide Tatsumi, and Tetsuo Takehara
    • Organizer
      The 25th Conference of the Asian Pacific Association for the Study of the Liver
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-02-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Significance of connective tissue growth factor in hepatocellular carcinoma and its potential for therapeutic target2015

    • Author(s)
      Yuki Makino, Hayato Hikita, Tsukasa Kawaguchi, Takahiro Kodama, Minoru Shigekawa, Yugo Kai, Yasutoshi Nozaki, Tasuku Nakabori, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Takuya Miyagi, Tomohide Tatsumi, Tetsuo Takehara
    • Organizer
      The 66th Annual Meeting of the American Association for the study of Liver Disease
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of connective tissue growth factor in hepatocellular carcinoma2015

    • Author(s)
      Yuki Makino, Hayato Hikita, Yasutoshi Nozaki, Kenji Ikezawa, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Tomohide Tatsumi, Tetsuo Takehara
    • Organizer
      日本癌学会第74回総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Significance of connective tissue growth factor with the malignant grade of hepatocellular carcinoma and its potential for therapeutic target2015

    • Author(s)
      Yuki Makino, Hayato Hikita, and Tetsuo Takehara
    • Organizer
      第19回日本肝臓学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肝細胞癌におけるRas経路とCTGFの意義・新規治療標的としての可能性2015

    • Author(s)
      牧野祐紀 疋田隼人 川口司 甲斐優吾 野崎泰俊 中堀輔 齋藤義修 田中聡司 重川稔 小玉尚宏 阪森亮太郎 宮城琢也 巽智秀 竹原徹郎
    • Organizer
      第51回日本肝臓学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2015-05-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Significance of connective tissue growth factor in hepatocellular carcinoma2015

    • Author(s)
      Yuki Makino, Hayato Hikita, Tsukasa Kawaguchi, Takahiro Kodama, Minoru Shigekawa, Yugo Kai, Yasutoshi Nozaki, Tasuku Nakabori, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Takuya Miyagi, Tomohide Tatsumi, Tetsuo Takehara
    • Organizer
      The 3rd JSGE International Topic Conference
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2015-04-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Significance of Ras pathway and connective tissue growth factor in the development of hepatocellular carcinoma2014

    • Author(s)
      Yuki Makino, Hayato Hikita, Tsukasa Kawaguchi, Takahiro Kodama, Minoru Shigekawa, Yugo Kai, Yasutoshi Nozaki, Tasuku Nakabori, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Takuya Miyagi, Tomohide Tatsumi, Tetsuo Takehara
    • Organizer
      Significance of Ras pathway and connective tissue growth factor in the development of hepatocellular carcinoma
    • Place of Presentation
      Boston, USA
    • Year and Date
      2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 肝発癌・進展におけるRas経路の活性化とCTGF2014

    • Author(s)
      牧野祐紀 疋田隼人 竹原徹郎
    • Organizer
      第18回日本肝臓学会大会(JDDW2014)
    • Place of Presentation
      神戸
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Significance of connective tissue growth factor (CTGF) for the oncogenesis and progression of hepatocellular carcinoma2014

    • Author(s)
      Yuki Makino, Hayato Hikita, Tsukasa Kawaguchi, Yugo Kai, Yasutoshi Nozaki, Tasuku Nakabori, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Takuya Miyagi, Tomohide Tatsumi, Tetsuo Takehara
    • Organizer
      日本癌学会第73回総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi