Development of animal model mimicking ornithine transcarbamylase deficiency

• Project/Area Number: 26670383
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Hiroshima University
• Principal Investigator: CHAYAMA KAZUAKI 広島大学, 医歯薬保健学研究院(医), 教授 (00211376)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed(Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost : ¥2,800,000、Indirect Cost : ¥840,000); Fiscal Year 2014 : ¥3,640,000 (Direct Cost : ¥2,800,000、Indirect Cost : ¥840,000)
• Keywords: ｹﾞﾉﾑ / ゲノム

Outline of Final Research Achievements

There are several liver diseases caused by genetic disorder such as Wilson’s disease, hereditary hemochromatosis and so on. One of such diseases, ornithine transcarbamylase (OTC) deficiency is caused by mutations in the OTC gene, which is located on the X chromosome. Knockout mouse is often used to study such disorders. However, the metabolism of mouse is different from that of humans. We attempted to develop a mouse model of OTC deficiency using human hepatocyte chimeric mouse. Using genome editing technology we will be able to ablate the gene to make OTC deficient liver cells. We confirmed that TALEN which cut target gene in liver cancer cell lines actually worked and also developed a method to confirm if the gene of interest had actually been ablated. This mouse model will be useful to study human liver situation in mice and will be useful to develop therapy for this disorder.

Research Products

• [Journal Article] Differences in serum microRNA profiles in hepatitis B and C virus infection. (2015)
  • Author(s): Akamatsu S, Hayes CN, Tsuge M, Miki D, Akiyama R, Abe H, Ochi H, Hiraga N, Imamura M, Takahashi S, Aikata H, Kawaoka T, Kawakami Y, Ohishi W, Chayama K.
  • Journal Title: J Infect. Volume : 70 Pages : 273-287
  • DOI: 10.1016/j.jinf.2014.10.017
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stereotactic body radiotherapy for patients with small hepatocellular carcinoma ineligible for resection or ablation therapies. (2015)
  • Author(s): Kimura T, Aikata H, Takahashi S, Takahashi I, Nishibuchi I, Doi Y, Kenjo M, Murakami Y, Honda Y, Kakizawa H, Awai K, Chayama K, Nagata Y.
  • Journal Title: Hepatol Res. Volume : 45 Pages : 378-386
  • DOI: 10.1111/hepr.12359
  • Peer Reviewed / Open Access
• [Journal Article] The effects of bisphosphonate zoledronic acid in hepatocellular carcinoma, depending on mevalonate pathway. (2015)
  • Author(s): Honda Y, Takahashi S, Zhang Y, Ono A, Murakami E, Shi N, Kawaoka T, Miki D, Tsuge M, Hiraga N, Abe H, Ochi H, Imamura M, Aikata H, Chayama K.
  • Journal Title: J Gastroenterol Hepatol. Volume : 30 Pages : 619-627
  • DOI: 10.1111/jgh.12715
  • Peer Reviewed / Open Access
• [Journal Article] Integrated analysis of whole genome and transcriptome sequencing reveals diverse transcriptomic aberrations driven by somatic genomic changes in liver cancers. (2015)
  • Author(s): Shiraishi Y, Fujimoto A, et al.
  • Journal Title: PLoS One Volume : 9
  • DOI: 10.1371/journal.pone.0114263
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hepatitis C virus core protein suppresses mitophagy by interacting with parkin in the context of mitochondrial depolarization. (2014)
  • Author(s): Hara Y, Yanatori I, Ikeda M, Kiyokage E, Nishina S, Tomiyama Y, Toida K, Kishi F, Kato N, Imamura M, Chayama K, Hino K.
  • Journal Title: Am J Pathol. 2014 Volume : 184 Pages : 3026-39
  • DOI: 10.1016/j.ajpath.2014.07.024
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interleukin 28B polymorphism predicts interferon plus ribavirin treatment outcome in patients with hepatitis C virus-related liver cirrhosis: A multicenter retrospective study in Japan. (2014)
  • Author(s): Shakado S, Sakisaka S, Okanoue T, Chayama K, Izumi N, Toyoda J, Tanaka E, Ido A, Takehara T, Yoshioka K, Hiasa Y, Nomura H, Seike M, Ueno Y, Kumada H.
  • Journal Title: Hepatol Res Volume : 44 Pages : 983-992
  • DOI: 10.1111/hepr.12280
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of early response to hepatic arterial infusion chemotherapy in patients with advanced hepatocellular carcinoma using the combination of RECIST and tumor markers. (2014)
  • Author(s): Miyaki D, Kawaoka T, Aikata H, Kan H, Fujino H, Fukuhara T, Kobayashi T, Naeshiro N, Honda Y, Tsuge M, Hiramatsu A, Imamura M, Hyogo H, Kawakami Y, Yoshimatsu R, Yamagami T, Awai K, Chayama K.
  • Journal Title: J Gastroenterol Hepatol. Volume : 30 Pages : 726-32
  • DOI: 10.1111/jgh.12821
  • Peer Reviewed / Open Access
• [Journal Article] Impact of alloimmune T cell responses on hepatitis C virus replication in liver transplant recipients. (2014)
  • Author(s): Tanaka Y, Ohira M, Tashiro H, Imamura M, Chayama K, Ohdan H.
  • Journal Title: Hum Immunol. Volume : 75 Pages : 1259-1267
  • DOI: 10.1016/j.humimm.2014.09.006
  • Peer Reviewed / Open Access
• [Journal Article] Emerging treatments for chronic hepatitis C. (2014)
  • Author(s): Hayes CN, Chayama K.
  • Journal Title: J Formos Med Assoc. Volume : 114 Pages : 204-215
  • DOI: 10.1016/j.jfma.2014.09.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Case reports of portal vein thrombosis and bile duct stenosis after stereotactic body radiation therapy for hepatocellular carcinoma. (2014)
  • Author(s): Takahashi S, Kimura T, Kenjo M, Nishibuchi I, Takahashi I, Takeuchi Y, Doi Y, Kaneyasu Y, Murakami Y, Honda Y, Aikata H, Chayama K, Nagata Y.
  • Journal Title: Hepatol Res. Volume : 44
  • DOI: 10.1111/hepr.12241
  • Peer Reviewed / Open Access
• [Journal Article] Endocannabinoid CB1 antagonists inhibit hepatitis C virus production, providing a novel class of antiviral host-targeting agents. (2014)
  • Author(s): Shahidi M, Tay ES, Read SA, Ramezani-Moghadam M, Chayama K, George J, Douglas MW.
  • Journal Title: J Gen Virol. Volume : 95 Pages : 2468-2479
  • DOI: 10.1099/vir.0.067231-0
• [Journal Article] Upper limit of normal serum alanine aminotransferase levels in Japanese subjects． (2014)
  • Author(s): Tanaka K，Hyogo H，Ono M，Takahashi H，Kitajima Y，Ono N，Eguchi T，Fujimoto K，Chayama K，Saibara T，Anzai K，Eguchi Y，and the Japan Study Group of Non-alcoholic Fatty Liver disease．
  • Journal Title: Hepatol Res Volume : 44(12) Pages : 1196-1207
  • DOI: 10.1111/hepr.12293
  • Peer Reviewed / Open Access
• [Journal Article] Type 2 diabetes mellitus is associated with the fibrosis severity in patients with nonalcoholic fatty liver disease in a large retrospective cohort of Japanese patients. (2014)
  • Author(s): Nakahara T, Hyogo H, Yoneda M, Sumida Y, Eguchi Y, Fujii H, Ono M, Kawaguchi T, Imajo K, Aikata H, Tanaka S, Kanemasa K, Fujimoto K, Anzai K, Saibara T, Sata M, Nakajima A, Itoh Y, Chayama K, Okanoue T
  • Journal Title: J Gastroenterol Volume : 49(11) Pages : 1477-1484
  • DOI: 10.1007/s00535-013-0911-1
  • Peer Reviewed / Open Access
• [Journal Article] Effect of vitamin D supplementation on pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b: a randomized controlled trial (2014)
  • Author(s): Yokoyama S. Takahashi S. Kawakami Y. Hayes C N. Kohno H. Kohno H. Tsuji K. Aisaka Y. Kira S. Yamashina K. Nonaka M. Moriya T. Kitamoto M. Aimitsu S. Nakanishi T. Kawakami H, and Chayama K
  • Journal Title: J Viral Hepat Volume : 21 Pages : 348-56
  • DOI: 10.1111/jvh.12146
  • Peer Reviewed / Open Access
• [Journal Article] Long-term outcome of patients with gastric varices treated by balloon-occluded retrograde transvenous obliteration. (2014)
  • Author(s): Naeshiro N, Aikata H, Kakizawa H, Hyogo H, Kan H, Fujino H, Kobayashi T, Fukuhara T, Honda Y, Ohno A, Miyaki D, Kawaoka T, Tsuge M, Hiraga N, Hiramatsu A, Imamura M, Kawakami Y, Takahashi S, Awai K, Chayama K.
  • Journal Title: J Gastroenterol Hepatol. Volume : 29 Pages : 1035-1042
  • DOI: 10.1111/jgh.12508
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy and safety of sitagliptin for the treatment of nonalcoholic fatty liver disease with type 2 diabetes mellitus. (2014)
  • Author(s): Fukuhara T, Hyogo H, Ochi H, Fujino H, Kan H, Naeshiro N, Honda Y, Miyaki D, Kawaoka T, Tsuge M, Hiramatsu A, Imamura M, Kawakami Y, Aikata H, Chayama K.
  • Journal Title: Hepatogastroenterology. Volume : 61 Pages : 323-328
  • Peer Reviewed
• [Journal Article] Noninvasive assessment of liver steatosis in nonalcoholic fatty liver disease. (2014)
  • Author(s): Kan H. Kimura Y. Hyogo H. Fukuhara T. Fujino H. Naeshiro N. Honda Y. Kawaoka T. Tsuge M. Hiramatsu A. Imamura M. Kawakami Y. Aikata H. Ochi H. Arihiro K, and Chayama K
  • Journal Title: Hepatol Res Volume : 印刷中
  • DOI: 10.1111/hepr.12330
  • Peer Reviewed / Open Access
• [Journal Article] interferon lambda 4/interleukin-28B haplotype structure and its impact on susceptibility to hepatitis C virus and treatment response in the Japanese population (2014)
  • Author(s): Ochi H, Miki D, Hayes CN, Abe H, Hayashida Y, Kubo M, Chayama K
  • Journal Title: Journal of General Virology Volume : 95巻 Pages : 1297-306
  • DOI: 10.1099/vir.0.060103-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The association of insomnia with gastroesophageal reflux symptoms in biopsy-proven nonalcoholic fatty liver disease. (2014)
  • Author(s): Taketani H, Sumida Y, Tanaka S, Imajo K, Yoneda M, Hyogo H, Ono M, Fujii H, Eguchi Y, Kanemasa K, Chayama K, Itoh Y, Yoshikawa T, Saibara T, Fujimoto K, Nakajima A
  • Journal Title: J Gastroenterol Volume : 49(7) Pages : 1163-1174
  • DOI: 10.1007/s00535-013-0871-5
  • Peer Reviewed / Open Access
• [Journal Article] Percutaneous transvenous embolization for portosystemic shunts associated with encephalopathy: Long-term outcomes in 14 patients. (2014)
  • Author(s): Naeshiro N, Kakizawa H, Aikata H, Kan H, Fujino H, Fukuhara T, Kobayashi T, Honda Y, Miyaki D, Kawaoka T, Tsuge M, Hiramatsu A, Imamura M, Kawakami Y, Hyogo H, Ishikawa M, Awai K, Chayama K.
  • Journal Title: Hepatol Res. Volume : 44 Pages : 740-749
  • DOI: 10.1111/hepr.12181
  • Peer Reviewed / Open Access
• [Journal Article] Pilot study of stereotactic body radiation therapy combined with transcatheter arterial chemoembolization for small hepatocellular carcinoma. (2014)
  • Author(s): Honda Y, Kimura T, Aikata H, Nakahara T, Naeshiro N, Tanaka M, Miyaki D, Nagaoki Y, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Ishikawa M, Kakizawa H, Kenjo M, Awai K, Nagata Y, Chayama K.
  • Journal Title: Hepatogastroenterology. Volume : 61 Pages : 31-36
  • Peer Reviewed
• [Journal Article] Virologic escape in HCV genotype 1-infected patients receiving daclatasvir plus ribavirin and peginterferon alfa-2a or alfa-2b. (2014)
  • Author(s): McPhee F. Hernandez D. Zhou N. Yu F. Ueland J. Monikowski A. Chayama K. Toyota J. Izumi N. Yokosuka O. Kawada N. Osaki Y. Hughes E A. Watanabe H. Ishikawa H, and Kumada H
  • Journal Title: Antivir Ther Volume : 印刷中 Pages : 479-490
  • DOI: 10.3851/imp2729
  • Peer Reviewed
• [Journal Article] Quantitative effect of natural killer-cell licensing on hepatocellular carcinoma recurrence after curative hepatectomy (2014)
  • Author(s): Tanimine N, Tanaka Y, Kobayashi T, Tashiro H, Miki D, Imamura M, Aikata H, Tanaka J, Chayama K, Ohdan H.
  • Journal Title: Cancer Immunol Res Volume : 2(12) Pages : 1142-1147
  • DOI: 10.1158/2326-6066.cir-14-0091
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Predictive value of the IFNL4 polymorphism on outcome of telaprevir, peginterferon, and ribavirin therapy for older patients with genotype 1b chronic hepatitis C. (2014)
  • Author(s): Fujino H, Imamura M, Nagaoki Y, Kawakami Y, Abe H, Hayes CN, Kan H, Fukuhara T, Kobayashi T, Masaki K, Ono A, Nakahara T, Honda Y, Naeshiro N, Urabe A, Yokoyama S, Miyaki D, Murakami E, Kawaoka T, Hiraga N, Tsuge M, Hiramatsu A, Hyogo H, Aikata H, Takahashi S, Miki D, Ochi H, Ohishi W, Chayama K
  • Journal Title: J Gastroenterol. Volume : 49 Pages : 1548-1556
  • DOI: 10.1007/s00535-013-0924-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simple scoring system for predicting cirrhosis in nonalcoholic fatty liver disease. (2014)
  • Author(s): Kessoku T, Ogawa Y, Yoneda M, Imajo K, Sumida Y, Eguchi Y, Fujii H, Hyogo H, Ono M, Suzuki Y, Kawaguchi T, Chayama K, Tanaka S, Fujimoto K, Anzai K, Saibara T, Sata M, Itoh Y, Nakajima A, Okanoue T
  • Journal Title: World J Gastroenterol Volume : 20（29） Pages : 10108-10114
  • DOI: 10.3748/wjg.v20.i29.10108
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interferon lambda 4 polymorphism affects on outcome of telaprevir, pegylated interferon and ribavirin combination therapy for chronic hepatitis C. (2014)
  • Author(s): Nagaoki Y, Imamura M, Kawakami Y, Kan H, Fujino H, Fukuhara T, Kobayashi T, Ono A, Nakahara T, Naeshiro N, Urabe A, Yokoyama S, Miyaki D, Murakami E, Kawaoka T, Tsuge M, Hiramatsu A, Aikata H, Takahashi S, Hayes CN, Ochi H, Chayama K, Hiroshima Liver Study G.
  • Journal Title: Hepatol Res. Volume : 44
  • DOI: 10.1111/hepr.12336
  • Peer Reviewed / Open Access
• [Journal Article] New insight into the enhanced effect of pegylated interferon-α. (2014)
  • Author(s): Abe H, Hayes CN, Chayama K.
  • Journal Title: Hepatology. Volume : 60 Pages : 1435-1437
  • DOI: 10.1002/hep.27269
  • Peer Reviewed / Open Access
• [Journal Article] Human microRNA hsa-miR-1231 suppresses hepatitis B virus replication by targeting core mRNA. (2014)
  • Author(s): Kohno T, Tsuge M, Murakami E, Hiraga N, Abe H, Miki D, Imamura M, Ochi H, Hayes CN, Chayama K.
  • Journal Title: J Viral Hepat. Volume : 21
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Association of polymorphisms in GCKR and TRIB1 with nonalcoholic fatty liver disease and metabolic syndrome traits. (2014)
  • Author(s): Kitamoto A, Kitamoto T, Nakamura T, Ogawa Y, Yoneda M, Hyogo H, Ochi H, Mizusawa S, Ueno T, Nakao K, Sekine A, Chayama K, Nakajima A, Hotta K.
  • Journal Title: Endocr J. Volume : 61 Pages : 683-689
  • Peer Reviewed / Open Access
• [Journal Article] Daclatasvir plus asunaprevir for chronic HCV genotype 1b infection (2014)
  • Author(s): Kumada H, Suzuki Y, Ikeda K, Toyota J, Karino Y, Chayama K, Kawakami Y, Ido A, Yamamoto K, Takaguchi K, Izumi N, Koike K, Takehara T, Kawada N, Sata M, Miyagoshi H, Eley T, McPhee F, Damokosh A, Ishikawa H, and Hughes E
  • Journal Title: Hepatology Volume : (in press) Pages : 2083-2091
  • DOI: 10.1002/hep.27113
  • Peer Reviewed / Open Access
• [Journal Article] Targeted next-generation sequencing and fine linkage disequilibrium mapping reveals association of PNPLA3 and PARVB with the severity of nonalcoholic fatty liver disease. (2014)
  • Journal Title: Journal of human genetics Volume : 59 Issue : 5 Pages : 241-246
  • Publisher: Nature Publisheng Group
  • DOI: 10.1038/jhg.2014.17
  • PMID: 24621583
  • Peer Reviewed
• [Journal Article] Ultradeep sequencing study of chronic hepatitis C virus genotype 1 infection in patients treated with daclatasvir, peginterferon, and ribavirin (2014)
  • Author(s): Murakami, E. Imamura, M. Hayes, C. N. Abe, H. Hiraga, N. Honda, Y. Ono, A. Kosaka, K. Kawaoka, T. Tsuge, M. Aikata, H. Takahashi, S. Miki, D. Ochi, H. Matsui, H. Kanai, A. Inaba, T. McPhee, F. Chayama, K.
  • Journal Title: Antimicrob Agents Chemother Volume : 58 Pages : 2105-2112
  • DOI: 10.1128/aac.02068-13
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A comparative study of 4 Fr versus 6 Fr nasobiliary drainage catheters: a randomized, controlled trial. (2014)
  • Author(s): Ishigaki T. Sasaki T. Serikawa M. Minami T. Okazaki A. Yukutake M. Ishii Y. Kosaka K. Mouri T. Yoshimi S, and Chayama K
  • Journal Title: J Gastroenterol Hepatol Volume : 29 Pages : 653-9
  • DOI: 10.1111/jgh.12427
  • Peer Reviewed / Open Access
• [Journal Article] Transarterial therapy of hepatocellular carcinoma fed by the right renal capsular artery. (2014)
  • Author(s): Ishikawa M, Yamagami T, Kakizawa H, Hieda M, Toyota N, Fukumoto W, Kajiwara K, Yoshimatsu R, Aikata H, Chayama K, Awai K.
  • Journal Title: J Vasc Interv Radiol. Volume : 25 Pages : 389-395
  • DOI: 10.1016/j.jvir.2013.11.009
  • Peer Reviewed
• [Journal Article] Aryl hydrocarbon receptor plays protective roles in ConA-induced hepatic injury by both suppressing IFN-Y expression and inducing IL-22 (2014)
  • Author(s): Abe H, Kimura A, Tsuruta S, Fukaya T, Sakaguchi R, et al.
  • Journal Title: Int Immunol. Volume : 26 Pages : 129-137
  • DOI: 10.1093/intimm/dxt049
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Non-invasive liver fibrosis score calculated by combination of virtual touch tissue quantification and serum liver functional tests in chronic hepatitis C patients (2014)
  • Author(s): Takaki S. Kawakami Y. Miyaki D. Nakahara T. Naeshiro N. Murakami E. Tanaka M. Honda Y. Yokoyama S. Nagaoki Y. Kawaoka T. Hiramatsu A. Tsuge M. Hiraga N. Imamura M. Hyogo H. Aikata H. Takahashi S. Arihiro K, and Chayama K
  • Journal Title: Hepatol Res Volume : 44 Pages : 280-7
  • DOI: 10.1111/hepr.12129
  • Peer Reviewed / Open Access
• [Journal Article] Long-term elimination of hepatitis C virus from human hepatocyte chimeric mice after interferon-γ gene transfer. (2014)
  • Journal Title: Human gene therapy. Clinical development Volume : 25 Issue : 1 Pages : 28-39
  • Publisher: Mary Ann Liebert, Inc.
  • DOI: 10.1089/humc.2013.066
  • PMID: 24279674
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical outcome and prognostic factors of patients with hepatocellular carcinoma and extrahepatic metastasis treated with sorafenib. (2014)
  • Author(s): Kawaoka T. Aikata H. Kan H. Fujino H. Fukuhara T. Kobayashi T. Naeshiro N. Miyaki D. Hiramatsu A. Imamura M. Kawakami Y. Hyogo H, and Chayama K
  • Journal Title: Hepatol Res Volume : 印刷中 Pages : 1320-1328
  • DOI: 10.1111/hepr.12307
  • Peer Reviewed / Open Access
• [Journal Article] Letter : Pitavastatin supplementation of PEG-IFN/ribavirin improves sustained virological response against HCV (2014)
  • Author(s): Yokoyama S, Kawakami Y, and Chayama K
  • Journal Title: Aliment Pharmacol Ther Volume : 39 Pages : 4-443
  • DOI: 10.1111/apt.12605
  • Peer Reviewed / Open Access
• [Journal Article] Serum asymmetric dimethylarginine levels are independently associated with procollagen III N-terminal peptide in nonalcoholic fatty liver disease patients. (2014)
  • Author(s): Hyogo H. Yamagishi S. Maeda S. Fukami K. Ueda S. Okuda S. Nakahara T. Kimura Y. Ishitobi T, and Chayama K
  • Journal Title: Clin Exp Med Volume : 14 Pages : 45-51
  • DOI: 10.1007/s10238-012-0223-4
  • Peer Reviewed / Open Access
• [Journal Article] A randomized trial of daclatasvir with peginterferon alfa-2b and ribavirin for HCV genotype 1 Infection. (2014)
  • Author(s): Suzuki F, Toyoa J, Ikeda K, Chayama K, Mochida S, Hayashi N, Ishikawa H, Miyagoshi H, Hu W, McPhee F, Hughes
  • Journal Title: Antiviral Ther Volume : in press Pages : 491-499
  • DOI: 10.3851/imp2730
  • Peer Reviewed
• [Journal Article] Two patients treated with pegylated interferon/ribavirin/telaprevir triple therapy for recurrent hepatitis C after living donor liver transplantation. (2014)
  • Author(s): Kawaoka T. Takahashi S. Tatsukawa Y. Hiramatsu A. Hiraga N. Miki D. Tsuge M. Imamura M. Kawakami Y. Aikata H. Ochi H. Ishiyama K. Ide K. Tashiro H. Ohdan H, and Chayama K
  • Journal Title: Hepatol Res Volume : 印刷中 Pages : 1259-1264
  • DOI: 10.1111/hepr.12296
  • Peer Reviewed / Open Access
• [Journal Article] Serum interleukin-6 associated with hepatocellular carcinoma risk: a nested case-control study (2014)
  • Author(s): Waka Ohishi, John B. Cologne, Saeko Fujiwara, Gen Suzuki, Tomonori Hayashi, Yasuharu Niwa, Masazumi Akahoshi, Keiko Ueda, Masataka Tsuge, Kazuaki Chayama
  • Journal Title: International Journal of Cancer Volume : 134巻 Pages : 154-163
  • DOI: 10.1002/ijc.28337
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nonalcoholic fatty liver disease and cardiovascular disease. (2014)
  • Author(s): Hyogo H, Chayama K, Yamagishi S.
  • Journal Title: Curr Pharm Des. Volume : 20 Pages : 2403-2411
  • DOI: 10.2174/13816128113199990476
  • Peer Reviewed
• [Journal Article] Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system (2014)
  • Author(s): Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  • Journal Title: Sci Rep Volume : 4 Pages : 5400-5400
  • DOI: 10.1038/srep05400
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Telaprevir is effective given every 12 h at 750 mg with pegylated interferon-alpha2b and ribavirin to Japanese patients with HCV-1b IL28B rs8099917 TT. (2014)
  • Author(s): Kawakami Y, Suzuki F, Karino Y, Toyota J, Kumada H, Chayama K.
  • Journal Title: Antivir Ther. Volume : 19 Pages : 277-285
  • DOI: 10.3851/imp2706
  • Peer Reviewed
• [Journal Article] Smoking cessation reverses DNA double-strand breaks in human mononuclear cells. (2014)
  • Author(s): Ishida M
  • Journal Title: PLoS One Volume : 9 Pages : 1-9
  • DOI: 10.1371/journal.pone.0103993
  • Peer Reviewed / Open Access
• [Journal Article] Hepatitis C virus (HCV) reactivation caused by steroid therapy for dermatomyositis. (2014)
  • Author(s): Mori N, Imamura M, Takaki S, Araki T, Hayes NC, Aisaka Y, Chayama K.
  • Journal Title: Intern Med. Volume : 53 Pages : 2689-2693
  • DOI: 10.2169/internalmedicine.53.3194
  • Peer Reviewed / Open Access
• [Journal Article] Differences in K-ras and mitochondrial DNA mutations and microsatellite instability between colorectal cancers of Vietnamese and Japanese patients (2014)
  • Journal Title: BMC Gastroenterology Volume : 14 Pages : 203
  • Publisher: BioMed Central
  • DOI: 10.1186/s12876-014-0203-0
  • PMID: info:pmid/25433803
  • Year and Date: 2014-11-30
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Hepatitis B mouse model involving CTL (2015)
  • Author(s): 茶山一彰
  • Organizer: 肝炎部会
  • Place of Presentation: 台北（台湾）
  • Year and Date: 2015-01-25
• [Presentation] 肝硬変の新たなマネージメント (2014)
  • Author(s): 茶山一彰
  • Organizer: 第102回日本消化器病学会中国支部例会
  • Place of Presentation: 広島
  • Year and Date: 2014-11-29
• [Presentation] All-oral Dual Combination of Daclatasvir plus Asunaprevir Compared with Telaprevir plus Peginterferon Alfa/Ribavirin in Treatment-maive Japanese Patients ChronicallyInfected with HCV Genotype 1b:Results from a Phase 3 Study (2014)
  • Author(s): 茶山一彰
  • Organizer: The 65nd Annual Meeting of American Association for the Study of Liver Diseases 2014(AASLD)
  • Place of Presentation: ボストン(アメリカ)
  • Year and Date: 2014-11-11
• [Presentation] 肝炎ウイルス感染と肝細胞癌 (2014)
  • Author(s): 茶山一彰
  • Organizer: 日本消化器関連学会機構(JDDW)2014
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-26
• [Presentation] C型肝炎治療の新展開と到達点 (2014)
  • Author(s): 茶山一彰
  • Organizer: JDDW2014第18回日本肝臓学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-23 – 2014-10-24
• [Presentation] 肝癌に対する経カテーテル治療におけるDEB-TACEの位置づけ (2014)
  • Author(s): 茶山一彰
  • Organizer: 第21回日本門脈圧亢進症学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-13
• [Presentation] C型慢性肝炎に対する治療の最前線 (2014)
  • Author(s): 茶山一彰
  • Organizer: 日本内科学会北陸支部 第62回生涯教育講演会
  • Place of Presentation: 金沢
  • Year and Date: 2014-06-22
• [Presentation] C型肝炎撲滅に向けた治療戦略 (2014)
  • Author(s): 茶山一彰
  • Organizer: 第101回日本消化器病学会中国支部例会
  • Place of Presentation: 岡山
  • Year and Date: 2014-06-21
• [Presentation] 講演1：DC Beadによる肝動脈化学塞栓療法 講演2：肝癌治療におけるイノベーション (2014)
  • Author(s): 茶山一彰
  • Organizer: 第50回日本肝癌研究会
  • Place of Presentation: 京都
  • Year and Date: 2014-06-05
• [Presentation] C型肝炎に対する最新の治療 (2014)
  • Author(s): 茶山一彰
  • Organizer: 第50回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-29
• [Presentation] プロテアーゼ阻害剤の知見と問題点 (2014)
  • Author(s): 茶山一彰
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-24
• [Remarks] 広島大学病院消化器・代謝内科ホームページ
  • URL: http://home.hiroshima-u.ac.jp/naika1/research_profile/liver_lab/
• [Patent(Industrial Property Rights)] 急性重症肝炎モデル非ヒト動物の作製方法、急性重症肝炎モデル非ヒト動物、劇症肝炎治療薬のスクリーニング方法および劇症肝炎治療薬 (2014)
  • Inventor(s): 茶山一彰
  • Industrial Property Rights Holder: 茶山一彰
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-217516
  • Filing Date: 2014-10-24
  • Application Year: 2014