Development of animal model mimicking ornithine transcarbamylase deficiency
Project/Area Number |
26670383
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Hiroshima University |
Principal Investigator |
CHAYAMA KAZUAKI 広島大学, 医歯薬保健学研究院(医), 教授 (00211376)
|
Project Period (FY) |
2014-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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Keywords | ゲノム / ゲノム |
Outline of Final Research Achievements |
There are several liver diseases caused by genetic disorder such as Wilson’s disease, hereditary hemochromatosis and so on. One of such diseases, ornithine transcarbamylase (OTC) deficiency is caused by mutations in the OTC gene, which is located on the X chromosome. Knockout mouse is often used to study such disorders. However, the metabolism of mouse is different from that of humans. We attempted to develop a mouse model of OTC deficiency using human hepatocyte chimeric mouse. Using genome editing technology we will be able to ablate the gene to make OTC deficient liver cells. We confirmed that TALEN which cut target gene in liver cancer cell lines actually worked and also developed a method to confirm if the gene of interest had actually been ablated. This mouse model will be useful to study human liver situation in mice and will be useful to develop therapy for this disorder.
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Report
(2 results)
Research Products
(64 results)
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[Journal Article] Differences in serum microRNA profiles in hepatitis B and C virus infection.2015
Author(s)
Akamatsu S, Hayes CN, Tsuge M, Miki D, Akiyama R, Abe H, Ochi H, Hiraga N, Imamura M, Takahashi S, Aikata H, Kawaoka T, Kawakami Y, Ohishi W, Chayama K.
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Journal Title
Journal of Infection
Volume: 70
Pages: 273-287
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Stereotactic body radiotherapy for patients with small hepatocellular carcinoma ineligible for resection or ablation therapies.2015
Author(s)
Kimura T, Aikata H, Takahashi S, Takahashi I, Nishibuchi I, Doi Y, Kenjo M, Murakami Y, Honda Y, Kakizawa H, Awai K, Chayama K, Nagata Y.
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Journal Title
Hepatol Res.
Volume: 45
Pages: 378-386
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of bisphosphonate zoledronic acid in hepatocellular carcinoma, depending on mevalonate pathway.2015
Author(s)
Honda Y, Takahashi S, Zhang Y, Ono A, Murakami E, Shi N, Kawaoka T, Miki D, Tsuge M, Hiraga N, Abe H, Ochi H, Imamura M, Aikata H, Chayama K.
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Journal Title
Journal of Gastroenterology and Hepatology
Volume: 30
Pages: 619-27
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Hepatitis C virus core protein suppr 1 esses mitophagy by interacting with Parkin in the context of mitochondrial depolarization.2014
Author(s)
Hara Y, Yanatori I, Ikeda M, Kiyokage E, Nishina S, Tomiyama Y, Toida K, Kishi F, Kato N, Imamura M, Chayama K, Hino K.
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Journal Title
Amer J Pathol
Volume: 184
Pages: 3026-3629
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Interleukin 28B polymorphism predicts interferon plus ribavirin treatment outcome in patients with hepatitis C virus-related liver cirrhosis: A multicenter retrospective study in Japan.2014
Author(s)
Shakado S, Sakisaka S, Okanoue T, Chayama K, Izumi N, Toyoda J, Tanaka E, Ido A, Takehara T, Yoshioka K, Hiasa Y, Nomura H, Seike M, Ueno Y, Kumada H.
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Journal Title
Hepatol Res.
Volume: 44
Pages: 983-992
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Evaluation of early response to hepatic arterial infusion chemotherapy in patients with advanced hepatocellular carcinoma using the combination of RECIST and tumor markers.2014
Author(s)
Miyaki D, Kawaoka T, Aikata H, Kan H, Fujino H, Fukuhara T, Kobayashi T, Naeshiro N, Honda Y, Tsuge M, Hiramatsu A, Imamura M, Hyogo H, Kawakami Y, Yoshimatsu R, Yamagami T, Awai K, Chayama K.
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Journal Title
J Gastroenterol Hepatol.
Volume: 30
Pages: 726-32
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Type 2 diabetes mellitus is associated with the fibrosis severity in patients with nonalcoholic fatty liver disease in a large retrospective cohort of Japanese patients.2014
Author(s)
Nakahara T, Hyogo H, Yoneda M, Sumida Y, Eguchi Y, Fujii H, Ono M, Kawaguchi T, Imajo K, Aikata H, Tanaka S, Kanemasa K, Fujimoto K, Anzai K, Saibara T, Sata M, Nakajima A, Itoh Y, Chayama K, Okanoue T
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Journal Title
J Gastroenterol.
Volume: 49(11)
Pages: 1477-1484
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long-term outcome of patients with gastric varices treated by balloon-occluded retrograde transvenous obliteration.2014
Author(s)
Naeshiro N, Aikata H, Kakizawa H, Hyogo H, Kan H, Fujino H, Kobayashi T, Fukuhara T, Honda Y, Ohno A, Miyaki D, Kawaoka T, Tsuge M, Hiraga N, Hiramatsu A, Imamura M, Kawakami Y, Takahashi S, Awai K, Chayama K.
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Journal Title
J Gastroenterol Hepatol.
Volume: 29
Pages: 1035-1042
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Efficacy and safety of sitagliptin for the treatment of nonalcoholic fatty liver disease with type 2 diabetes mellitus.2014
Author(s)
Fukuhara T, Hyogo H, Ochi H, Fujino H, Kan H, Naeshiro N, Honda Y, Miyaki D, Kawaoka T, Tsuge M, Hiramatsu A, Imamura M, Kawakami Y, Aikata H, Chayama K.
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Journal Title
Hepatogastroenterology.
Volume: 61
Pages: 323-328
Related Report
Peer Reviewed
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[Journal Article] The association of insomnia with gastroesophageal reflux symptoms in biopsy-proven nonalcoholic fatty liver disease.2014
Author(s)
Taketani H, Sumida Y, Tanaka S, Imajo K, Yoneda M, Hyogo H, Ono M, Fujii H, Eguchi Y, Kanemasa K, Chayama K, Itoh Y, Yoshikawa T, Saibara T, Fujimoto K, Nakajima A
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Journal Title
J Gastroenterol.
Volume: 49(7)
Pages: 1163-1174
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pilot study of stereotactic body radiation therapy combined with transcatheter arterial chemoembolization for small hepatocellular carcinoma.2014
Author(s)
Honda Y, Kimura T, Aikata H, Nakahara T, Naeshiro N, Tanaka M, Miyaki D, Nagaoki Y, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Ishikawa M, Kakizawa H, Kenjo M, Awai K, Nagata Y, Chayama K.
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Journal Title
Hepatogastroenterology.
Volume: 61
Pages: 31-36
Related Report
Peer Reviewed
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[Journal Article] Simple scoring system for predicting cirrhosis in nonalcoholic fatty liver disease.2014
Author(s)
Kessoku T, Ogawa Y, Yoneda M, Imajo K, Sumida Y, Eguchi Y, Fujii H, Hyogo H, Ono M, Suzuki Y, Kawaguchi T, Chayama K, Tanaka S, Fujimoto K, Anzai K, Saibara T, Sata M, Itoh Y, Nakajima A, Okanoue T
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Journal Title
World J Gastroenterol.
Volume: 20(29)
Pages: 10108-10114
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Interferon lambda 4 polymorphism affects on outcome of telaprevir, pegylated interferon and ribavirin combination therapy for chronic hepatitis C.2014
Author(s)
Nagaoki Y, Imamura M, Kawakami Y, Kan H, Fujino H, Fukuhara T, Kobayashi T, Ono A, Nakahara T, Naeshiro N, Urabe A, Yokoyama S, Miyaki D, Murakami E, Kawaoka T, Tsuge M, Hiramatsu A, Aikata H, Takahashi S, Hayes CN, Ochi H, Chayama K
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Journal Title
Hepatol Res.
Volume: (印刷中)
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Association of polymorphisms in GCKR and TRIB1 with nonalcoholic fatty liver disease and metabolic syndrome traits.2014
Author(s)
Kitamoto A, Kitamoto T, Nakamura T, Ogawa Y, Yoneda M, Hyogo H, Ochi H, Mizusawa S, Ueno T, Nakao K, Sekine A, Chayama K, Nakajima A, Hotta K.
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Journal Title
Endocr J.
Volume: 61
Pages: 683-689
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Daclatasvir plus asunaprevir for chronic HCV genotype 1b infection.2014
Author(s)
Kumada H, Suzuki Y, Ikeda K, Toyota J, Karino Y, Chayama K, Kawakami Y, Ido A, Yamamoto K, Takaguchi K, Izumi N, Koike K, Takehara T, Kawada N, Sata M, Miyagoshi H, Eley T, McPhee F, Damokosh A, Ishikawa H, and Hughes E
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Journal Title
Hepatology.
Volume: (in press)
Pages: 2083-2091
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Targeted next-generation sequencing and fine linkage disequilibrium mapping reveals association of PNPLA3 and PARVB with the severity of nonalcoholic fatty liver disease.2014
Author(s)
Kitamoto T, Kitamoto A, Yoneda M, Hyogo H, Ochi H, Mizusawa S, Ueno T, Nakao K, Sekine A, Chayama K, Nakajima A, Hotta K
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Journal Title
J Hum Genet.
Volume: 6
Pages: 241-246
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Ultradeep sequencing study of chronic hepatitis C virus genotype 1 infection in patients treated with daclatasvir, peginterferon, and ribavirin.2014
Author(s)
Murakami, E. Imamura, M. Hayes, C. N. Abe, H. Hiraga, N. Honda, Y. Ono, A. Kosaka, K. Kawaoka, T. Tsuge, M. Aikata, H. Takahashi, S. Miki, D. Ochi, H. Matsui, H. Kanai, A. Inaba, T. McPhee, F. Chayama, K.
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Journal Title
Antimicrobial Agents Chemotherapy
Volume: 58
Pages: 2105-12
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Transarterial therapy of hepatocellular carcinoma fed by the right renal capsular artery.2014
Author(s)
Ishikawa M, Yamagami T, Kakizawa H, Hieda M, Toyota N, Fukumoto W, Kajiwara K, Yoshimatsu R, Aikata H, Chayama K, Awai K.
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Journal Title
J Vasc Interv Radiol.
Volume: 25
Pages: 389-395
DOI
Related Report
Peer Reviewed
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[Journal Article] A randomized trial of daclatasvir with peginterferon alfa-2b and ribavirin for HCV genotype 1 infection.2014
Author(s)
Suzuki F, Toyoa J, Ikeda K, Chayama K, Mochida S, Hayashi N, Ishikawa H, Miyagoshi H, Hu W, McPhee F, Hughes
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Journal Title
Antivir Ther.
Volume: in press
Pages: 491-499
DOI
Related Report
Peer Reviewed
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